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The 2024 Thermo Fisher Scientific Microscopy Imaging Contest has concluded, and we have selected the winners. We received submissions from a diverse range of scientific disciplines, including developmental research, cancer research, and neuroscience. Each entry showcased the inherent beauty of their respective fields through captivating images and provided a unique perspective into their research project. Thank you to all our partcipants.
Thermo Fisher Scientific Microscopy Imaging Contest 2024 Winners
What does the image represent?
Congenital diaphragmatic hernia (CDH) is a rare malformation that mainly affects lung development, and its disease mechanisms are poorly understood. This image shows a lung organoid, which is a miniature replica of a human lung, grown from stem cells derived from patient with CDH. In my work I utilise fetal fluids, such as amniotic fluid, to isolate tissue-specific stem cells and grow them into organoids. Lung organoids provide a close-up view of how lungs work at a cellular level, and can be used to study congenital lung diseases and find potential therapies.
What are the experimental details?
Antibodies, reagents, and imaging platform used: Hoechst 33342, Thermo Fisher Scientific (Cat. No.: 62249 ); Alexa Fluor Phalloidin 488, Thermo Fisher Scientific (Cat. No.: A12379 ); Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647, Thermo Fisher Scientific (Cat. No.: A31573 ). Zeiss Confocal LSM710. Imaging software: Zeiss Zen.
What does the image represent?
To model and study the role glycans (sugars) play in driving Inflammatory Bowel Disease, we used a mouse model that expresses CreERT2 in the intestine. CreERT2 recognizes two LoxP sites flanking a genetic region of interest (C1galt1) and removes the loci from the mouse's genome upon induction with Tamoxifen (intraperitoneal injection). These mice are interbred with C3gnt-/- mice (global deletion) to create Double Knockout (DKO) mice that lack both Core 1 and Core 3 O-glycans when induced, which inhibits the mice from synthesizing mucins with complex sugars. The image is a section of the colon from this mouse model. Mice with a complete loss of both types of complex O-glycans can be confirmed via lack of Maackia amurensis lactin II (MALII) signal (red).
What are the experimental details?
Harvested colons were placed in Carnoy's fixative overnight at 4C and then processed. The tissue was stained with Maackia amurensis lectin II (MALII)-biotin diluted 1:500 for 1 hour at room temp. Slides were washed with PBST 3 times, then incubated with SYBR green (1:10,000 dilution), Wheat germ agglutinin (WGA)-350 blue (1:500 dilution), and Strep-594 red (1:500 dilution) for 1 hour at room temp. Slides were then washed again with PBST, then mounted with ProLong Glass Antifade Mountant. Blocking was done with the Endogenous Biotin-Blocking Kit following manufacturers recommendations.
Antibodies, reagents, and imaging platform used: Endogenous Biotin-Blocking Kit, Thermo Fisher (Cat. No.: E21390 ); ProLong Glass Antifade Mountant, Thermo Fisher (Cat. No.: P36982 ); Wheat Germ Agglutinin (WGA)-Alexa Fluor 350, Thermo Fisher (Cat. No.: W11263 ); Streptavidin, Alexa Fluor 594 conjugate, Thermo Fisher (Cat. No.: S11227 ); Maackia amurensis Lectin II (MAL II), Biotinylated, Vector Laboratories (Cat. No.: B-1265-1); SYBR Safe DNA Gel Stain, Thermo Fisher (Cat. No.: S33102 ); Thermo Fisher EVOS M5000.
What does the image represent?
The image shows a mouse neuroblastoma cath.a-differentiated (CAD) cell stained for F-actin (blue), microtubules (red), and mitochondria (green). It is part of a recent investigation where we demonstrated that prolonged depletion of F-actin increases the number of microtubules and causes them to become hyperacetylated, which alters the transport of mitochondria and other organelles.
My research focuses on studying the cytoskeleton crosstalk in neurodegenerative diseases. Cytoskeletal dynamics are essential for processes like axonal transport, synaptic plasticity, and cellular signaling, all of which are vital for healthy neuronal activity. In neurodegenerative diseases, disruptions in these processes can lead to neuronal dysfunction and cell death.
What are the experimental details?
The image was acquired with a super-resolution spinning-disk confocal microscope using optical photon reassignment. A Z-stack was acquired using a 100X objective and then deconvolved to further enhance the resolution.
Antibodies, reagents, and imaging platform used: Alexa Fluor 647 Phalloidin, Thermo Fisher Scientific (Cat. No. A22287); ProLong Diamond Anti-Fade, Thermo Fisher Scientific (Cat. No. P36961); Goat anti-mouse 488, Thermo Fisher Scientific (Cat. No. A11029); Goat anti-rabbit 568, Thermo Fisher Scientific (Cat. No. A11011); Magnesium chloride, Fisher Scientific (Cat. No. BP214); DMEM/F12 medium, Thermo Fisher Scientific (Cat. No. 11330-032); Cath.a-differentiated (CAD) cell (ATCC); rabbit polyclonal anti-alpha tubulin, Abcam (Cat. No. ab4074) and mouse monoclonal anti-TOM20 (4F3), Abcam (Cat. No. ab56783); Nikon CSU-W1 SoRa Spinning Disk Confocal Microscope.
What does the image represent?
In the last year of my PhD program, I received a diagnosis for a rare genetic kidney disease. This diagnosis, along with the required treatments, sparked a newfound interest in understanding the molecular mechanisms underlying kidney and glomerular diseases. As a result, I made a significant shift in my research focus from studying the cell biology of developmental cardiology/biology to investigating the genetic and molecular factors that contribute to chronic and acquired kidney and glomerular diseases.
The primary focus of my research lies in the investigation of a cellular structure called the primary cilium. This tiny, sensory organelle plays a crucial role in facilitating cell-to-cell communication and regulating various cellular processes such as differentiation, proliferation/apoptosis, cell survival, migration, and polarity. Although primary cilia were initially believed to be vestigial in nature, they were first discovered in the kidney over a century ago.
In the past five decades, significant advancements have been made in understanding the importance of primary cilia, as genetic mutations and structural damage to these organelles have been identified as causal factors in a group of diseases known as ciliopathies. This category of diseases continues to expand and encompasses a wide range of conditions, including obesity, osteoarthritis, certain forms of cancer, as well as congenital abnormalities and diseases such as Joubert Syndrome (JS), Bardet-Biedl syndrome (BBS), and the polycystic kidney diseases (PKD, ADPKD, ARPKD). The focus of my postdoctoral research training focuses on improving our understanding of the molecular mechanisms controlling glomerular (kidney) function, including those mediated through the primary cilium as well as mechanisms mediated by the sensory innervation of the glomerulus.
This tissue sample was stained to exhibit proteins fibronectin-1 and Sonic Hedgehog in glomeruli affected by minimal change disease glomerulopathy/podocytopathy.
What are the experimental details?
Formalin-fixed, paraffin-embedded human renal biopsy (FFPE) tissues were obtained from the Maine Health Biobank were processed and stained for structural markers found in healthy glomeruli. Primary antibodies were diluted to the appropriate working concentration and then applied to the tissue sections. A mountant with DAPI was applied to protect the fluorophore staining and mark the nuclei. The slides were imaged using Leica confocal microscopy at a resolution of 1024 x 1024 Hz. Z-stacks of the tissue sections were generated by capturing individual images at 0.2 micrometer z-thickness increments. A 63X oil objective with a zoom factor of 2.50 was used for imaging.
Antibodies, reagents, and imaging platform used: Anti-shh Polyclonal Antibody, Thermo Fisher Scientific (Cat. No.: MA5-51177 ); Alexa Fluor 488 goat anti-rabbit, Thermo Fisher Scientific (Cat. No.: A32731 ); Alexa Fluor 555 goat anti-mouse Thermo Fisher Scientific (Cat. No.: A21422 ). Fluormount with DAPI, anti-Fibronectin-1, Vendor: ProteinTech, Cat. No.: 15613-1-AP. Leica confocal microscope.
What is the most useful reagent for your experiment?
“The most useful reagents to these pivotal first imaging experiments were the secondary antibodies/Alexa fluorophores purchased through Invitrogen. Kidney tissue is known to be more difficult to work with using immunofluorescence due to a higher level of endogenously autofluorescence, a challenge that can be further exacerbated with the added influence of/variability of FFPE fixation/tissue preparation methods. Procuring secondary antibodies that are both robust and provide specific signal with minimal background ultimately enabled a clear visualization of human kidney/glomerular tissue in a manner that helped us characterize our experimental variables of interest as a part of designing a larger, ongoing study.”
- Lindsey Avery Fitzsimons
What does the image represent?
1 month old human neural network fibres (DcX positive, green) radiating from central neurosphere (Hoechst, blue) with GAP43 (red) positive cells.
This work is a part of Dr. Hodgett's laboratory at the Spinal Cord Repair Laboratory at the School of Human Sciences at UWA and Perron Institute for Neurological and Translational Science. Professor Hodgetts has over 20 years of expertise in cell based transplantation therapies. He has been devoted to this research since joining UWA in 1998, originally conducting research in neuromuscular diseases such as muscular dystrophy.
Since 2004 he has focused on the repair of the injured spinal cord using a variety of different stem cell, gene therapy, in vivo reprogramming, tissue engineering, neuroprotective and non-invasive strategies (such as repetitive transcranial magnetic stimulation and the use of infra-red/near infra-red light). His Spinal Cord Repair Laboratory acts as a core facility for other neuroscience researchers designed to promote neuro-regeneration into the setting of SCI toward preclinical studies. He is an academic coordinator for several undergraduate units at UWA, including Neuroscience, with supervision of over 35 Honours, 9 Masters and 20 PhD students.
He was the WA Representative for Australasian Neuroscience Society (2014-2017) and is co-chair for the ANS 2024 meeting in Perth. With previous NHMRC and current ARC funding, he has secured funds totaling nearly $5.5 million as Chief Investigator since 2001. He also works closely with national and international bio-artists (e.g., “cellF”, “Revivification”), as well as being scientific consultant for the internationally renowned “SymbioticA”.
What are the experimental details?
My research experiments use a variety of stem cell, gene therapy, bio-engineering and neuroprotection strategies to repair the injured spinal cord. These cells were stained and then imaged on a Nikon Eclipse Ti (Inverted) at x40 magnification. No image processing.
Antibodies, reagents, and imaging platform used: Doublecortin Monoclonal Antibody (2G5), Thermo Fisher Scientific (Cat. No.: MA5-17066); GAP43, Vendor: Thermo Fisher Scientific (Cat. No.: PA5-110781 replaced with PA5-34943 ); Nikon Eclipse Ti.
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1. Void where prohibited or restricted by law.
2. Contest begins at 12:00:00 AM (ET) on Monday, January 15, 2024 and ends at 11:59:59 PM (ET) on Wednesday 5/29/2024 (the “Contest Period”).
3. The “Microscopy Image Contest” (“Contest”) is sponsored by Thermo Fisher Scientific Inc., 81 Wyman Street, Waltham, Massachusetts (“Sponsor”).
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5. HOW TO ENTER. Complete all fields of the online registration form at https://www.thermofisher.com/us/en/home/global/forms/microscopy-imaging-contest.html (the “Registration Form”) and Contestants must fill out the form with the required information and submit their original microscopy image.
- About uploading the Entry Submission to our website:
- Delete all non-essential and/or proprietary information files from the Entry Submission.
- The Entry Submission file must be an image provided in JPG or PNG or TIFF with a resolution of greater than 300 DPI.
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- Limit three (3) entries per person or valid email address. If any question arises, an entry shall be attributed to the proper holder/owner of the email account used to submit the Registration Form. Duplicate entries will be disqualified. Any entry received with incomplete or illegible information will be deemed null and void and the sender will not be entered into the Contest.
6. QUALIFIED ENTRIES: Any entry received with incomplete, corrupted or illegible information will be deemed null, void and invalid. To be qualified for the Contest:
- You must complete the entire Registration Form; and you must submit an Entry Submission that: (a) includes your legal name, institute or place of employment, email address, phone number, city, postal/zip code, permission to contact you by phone number or email. Information about the original image must include the instrument that was used to capture the image, reagent(s) and/or antibody(ies) name(s), and data analysis software name ; (b) is wholly owned by you and is your original work, not copied from any other source; (c) has not been previously broadcast or otherwise distributed or disseminated in any media or format; (d) is not in the public domain; (e) is not in violation of or in conflict with the trademark, copyright, rights of privacy, rights of publicity or any other rights, of any kind or nature, of any other person or entity; (f) does not include any language or content that is, in Sponsor’s sole discretion, indecent, inappropriate or morally objectionable, or that is derogatory or disparaging. If the Entry Submission contains any material or elements that are not owned by the entrant and/or which are subject to the rights of third parties, the entrant is responsible for obtaining, prior to submission of the Entry Submission, any and all releases and consents necessary to permit the exhibition and use of the Entry Submission in the manner set forth in these Official Rules without any compensation. Entry Submissions not satisfying these criteria in any respect will be disqualified.
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7. PRIZE(S).
- Sponsor may award up to one (1) grand prize per winning image. Sponsor may award up to four (4) non-grand prizes per winning image. Sponsor reserves the right not to award any prize(s) if the criteria for entry is not met.
- Qualified entrants may win one of the following prizes:
- At the end of the Contest Period one grand prize winner will have their image selected to be featured in a coloring wall poster. The image will be converted into a black and white image that can be filled with a coloring pencil or marker. The winner will also receive a wall poster with the winning image with an approximate retail value of $US 14.00.
- At the end of the Contest Period one grand prize and four non-grand prize winners will receive reagents picked from a document from a list of specified Thermo Fisher Scientific reagents worth an approximate retail value of up to US $2000.
- At the end of the Contest Period one grand prize and four non-grand winners will receive a paper certificate commemorating their win in the Thermo Fisher Scientific Microscopy Imaging Contest and their bio, winning image, and winning image description will be featured on the https://www.thermofisher.com/us/en/home/c/a/microscopy-imaging-contest.html website.
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- United States and Canada (excluding Quebec) only: the Aspire Program will award 500 bonus points to Aspire members for successfully entering the contest. This offer is available only to members of the Aspire Member Program in the US and Canada (excluding Quebec). Healthcare professionals are not eligible to participate in the Member Program. The Aspire program will award 500 bonus points for successfully entering the contest by filling out the web form. Web forms will be deemed complete when all required fields are properly filled. Determination of form completeness will be at the sole discretion of Thermo Fisher Scientific. Please allow 10 business days for bonus points to credit to your account. Limit one submission per member. The completed contest web form must be received between February 1 and May 31, 2024. Cannot be combined with other discounts or promotions. All terms, conditions and restrictions of the Aspire program apply. View them here.
8. SELECTION AND NOTIFYING A WINNER
- Judging and Criteria:
- Sponsor may select one or more images. Sponsor will be a panel of 3 qualified judges comprised of one of the Sponsor’s employees with background in performing imaging experiments to serve as Contest judge(s) (the “Judge(s)”) from time to time and at its sole discretion. Judging will commence on 6/01/2024 and all winners will be selected on or before 6/15/2024.
- The Judge(s) judges will evaluate each entry and award up to the maximum number of points listed in each of the following categories:
A. Instrumentation (0-5 pts) examples judging criteria include:
for using an EVOS or CX7 instrument.
B. Reagents (0-10 pts) examples judging criteria include, but not limited to:
for using Invitrogen DAPI, Invitrogen NucBlu ReadyProbes, Invitrogen ReadyProbes Reagent, Invitrogen Cellular Fluorescent Imaging Reagents or Invitrogen Cellular Fluorescent Imaging probes, Invitrogen ReadyLabel labeling kit, Invitrogen Invitrogen pHrodo Intracellular pH Indicator Dyes, Click-iT EdU assay, and/or Invitrogen ViewRNA ISH Assay.
C. Antibodies (0-2 pts) examples judging criteria include, but not limited to:
using Invitrogen secondary antibodies conjugated to Invitrogen Alexa Fluor dyes, Invitrogen secondary antibodies conjugated to Invitrogen Alexa Fluor Plus dyes, Invitrogen primary antibodies.
D. Esthetic (0-30 pts) examples judging criteria include, but not limited to:
showing multiple colors, unique shapes created by using cells, location of cells
- Scoring: The qualified entry with the highest total score will be deemed the winner. In the event of a tie, the Entry Submissions involved in the tie will be re-judged by an additional judge who will serve as a tiebreaker and who will evaluate the Entry Submission using the same criteria specified above.
- The decision of [the Judges OR Sponsor] shall be final and binding, and not subject to legal recourse.
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- Within 14 days of notification, all winners must:
- acknowledge/claim prize; and
- provide Sponsor with a valid form of personal identification; and
- sign and return to Sponsor a release of liability, declaration of eligibility and where lawful, publicity consent agreement for the use of winner’s name, Survey responses, voice and/or likeness for the purpose of advertising, trade, or promotion without further compensation, unless prohibited by law.
- Failure to complete the above tasks within 14 days of notification will result in another winner being chosen. Sponsor can make no exceptions to this rule.
9. These Official Rules and the names of the winners will be available online at https://www.thermofisher.com/us/en/home/c/a/microscopy-imaging-contest.html on or after 6/15/2024 for at least 30 days.
10. GRANT OF RIGHTS. By submitting a Entry Submission, you grant to Sponsor an unlimited, non-exclusive, royalty free, assignable, worldwide, perpetual, license to use, reproduce, publish, distribute, transmit, exhibit, exploit, and license all parts of your qualified entry materials, including without limitation, the Entry Submission and any portions thereof in any format, by any and all means, uses and media, whether audio, print, audiovisual or otherwise, now or hereafter known for any and all purposes in Sponsor’s discretion. In addition, by participating in this Contest, you hereby consent to Sponsor’s use of your image and likeness in the Entry Submission singularly or in conjunction with other Entry Submissions for any and all advertising, publicity, commercial or other business purposes. You further consent to the reproduction and/or authorization by Sponsor to reproduce and use said Entry Submission for use in all domestic and foreign markets. Further, you understand that others, with or without the consent of Sponsor, may use and/or reproduce such Entry Submission, and you hereby release Sponsor and any of its associated or affiliated companies, their directors, officers, agents, employees and customers, and appointed advertising agencies, their directors, officers, agents and employees from all claims of every kind on account of such use.
11. RELEASE AND DISCLAIMERS
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- Thermo Fisher Affiliates shall not be responsible for any reason or event that might result in a prize not reaching a winner or not being suitable for use by entrant and no prize will be replaced.
12. DISPUTES:
- Any attempt by an entrant to deliberately damage any website or undermine the legitimate operations of the Contest is a violation of criminal and civil laws. In response to an attempt, Sponsor reserves the right to seek damages from such entrant to the fullest extent permitted by law and to disqualify the entrant from the Contest.
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