CRE BAG 2 fibroblasts. Oregon Green® 514 phalloidin and fluorescein phalloidin.

Photostability comparison of Oregon Green® 514 phalloidin (Cat. no. O7465, upper series) and fluorescein phalloidin (Cat. no. F432, lower series). CRE BAG 2 fibroblasts were fixed with formaldehyde, then permeabilized with acetone and stained with the fluorescent phallotoxin. Samples were illuminated continuously and viewed on a fluorescence microscope equipped with a fluorescein longpass optical filter set. Images acquired at 1, 10, 20 and 30 seconds after the start of illumination (left to right) demonstrate the superior photostability of the Oregon Green® 514 fluorophore.

Photostability comparison of Oregon Green® 514 phalloidin (Cat. no. O7465, upper series) and fluorescein phalloidin (Cat. no. F432, lower series). CRE BAG 2 fibroblasts were fixed with formaldehyde, then permeabilized with acetone and stained with the fluorescent phallotoxin. Samples were illuminated continuously and viewed on a fluorescence microscope equipped with a fluorescein longpass optical filter set. Images acquired at 1, 10, 20 and 30 seconds after the start of illumination (left to right) demonstrate the superior photostability of the Oregon Green® 514 fluorophore.

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