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The Thermo Scientific Orbitrap Astral mass spectrometer can help you rapidly and comprehensively capture the dynamic, temporal, and spatial complexity of biology but don’t just take our word for it. Discover what scientists in universities, hospitals and other research centers are saying about Orbitrap Astral MS.
Featured on-demand webinar
Faster Analysis of Human Proteomes Enabled by Narrow-Window Data-Independent Acquisition
In this on-demand webinar, Dr. Jesper V. Olsen of the University of Copenhagen will describe new methodologies and their application to biomedical research, including using narrow quadrupole isolation window data-independent acquisition (DIA) mode with short LC gradients.
Register to learn:
- Benefits of narrow-window data-independent acquisition (DIA)
- How to acquire comprehensive human proteomes within hours
- Optimize methods for low input samples, including single cells
Faster throughput
To accommodate larger sample groups, high throughput is essential. The Orbitrap Astral Mass Spectrometer achieves this by analyzing samples in just 8 minutes injection-to-injection, allowing acquisition of 180 samples per day. Each injection can identify 8,000+ proteins in a standard cell lysate sample, breaking through the bottleneck of sample analysis. This faster throughput enables the analysis of over 1.4 million cumulative protein group measurements across 180 samples in a single day, meaning a single Orbitrap Astral Mass Spectrometer can measure tens of thousands of samples in a year. This resets the scope of study scale, enhancing statistical power and revealing novel insights.
"The Orbitrap Astral mass spectrometer quickly and reproducibly delivers deeper and broader coverage of the plasma proteome with the accuracy and precision required to produce predictive information on the best treatment course for each of our patients in the clinic. That is precision medicine in action."
— Jennifer Van Eyk, Ph.D., Director, Basic Science Research, Barbra Streisand Women’s Heart Center Director, Advanced Clinical Biosystem Institute Center, Cedars-Sinai
Hear from Jennifer Van Eyk, Ph.D., Professor at Cedars-Sinai Medical Center and Joshua Coon, Ph.D., Professor of Biomolecular Chemistry at the University of Wisconsin-Madison, as they share insights on the exceptional data generated at high throughput by the Orbitrap Astral mass spectrometer.
Learn about the future of clinical proteomics from Jennifer Van Eyk, Ph.D., Professor at Cedars-Sinai Medical Center
Faster throughput for phosphoproteomics by Joshua Coon, Ph.D., Professor of Biomolecular Chemistry at the University of Wisconsin-Madison
Higher sensitivity
Due to the proteome's wide variability, both highly abundant and scarce proteins need measurement. The Orbitrap Astral Mass Spectrometer broadens this range, measuring proteins down to a level of just 200 copies per cell in bulk samples. For low-input samples, like single-cell proteomics, this instrument offers twice the throughput without sacrificing sensitivity, resulting in increased coverage and the analysis of more single-cells per day. These capabilities help decipher the heterogeneity of single cells to provide meaningful biological information.
"With the Orbitrap Astral mass spectrometer we were able to double the throughput for single cells, while also increasing the number of proteins per cell by 50%. This means we start picking up key cellular decision making proteins like transcription factors, epigenetic regulators and kinases to better understand the biological heterogeneity of single cells."
— Erwin Schoof, Ph.D., Associate Professor, Department of Biotechnology and Biomedicine, Section for Protein Science and Biotherapeutics, Cell Diversity Lab, Technical University of Denmark
Single-cell proteomics with the Orbitrap Astral MS by Erwin Schoof, Ph.D., Associate Professor, Department of Biotechnology and Biomedicine, Section for Protein Science and Biotherapeutics, Cell Diversity Lab, Technical University of Denmark
Novel approaches to single-cell proteomics by Nikolai Slavov, Ph.D., Founding Director of Parallel Squared Technology Institute and Professor at Northeastern University
Deeper coverage
Traditionally, achieving a comprehensive understanding of the entire proteome has been challenging. Cutting-edge methods can analyze roughly 12,000 proteins, but this demands extensive sample processing, multiple enzymes, and more than a day of LC-MS analysis time. Enter the Orbitrap Astral Mass Spectrometer, which swiftly achieves a proteome depth of over 12,000 proteins in just an hour from a single human cell lysate sample. Through multi-shot techniques, it reaches nearly whole proteome coverage of 15,000 proteins in 4.5 hours. The era of whole proteome sequencing has arrived.
"The Orbitrap Astral mass spectrometer enables routine acquisition of essentially complete Human proteomes with a throughput of up to eight proteomes per day—ten times higher than current state-of-the-art."
— Jesper Olsen, Ph.D., Deputy Center Director, Professor, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
Hear from Dr. Jesper V. Olsen of the University of Copenhagen and Sophia Steigerwald, Ph.D. Student at the Max Planck Institute of Biochemistry as they share insights on the deep whole proteome coverage of the Orbitrap Astral Mass Spectrometer.
Full proteome analysis by Jesper V. Olsen, Ph.D., Full Professor and Vice Director of NNF-CPR at University of Copenhagen
Deep visual proteomics by Sophia Steigerwald, Ph.D. Student at the Max Planck Institute of Biochemistry
Accurate and precise quantitation
Actionable insights require precise quantitative measurements, especially for complex biological processes. While some changes are clear cut, most involve subtle alterations in biomarkers like their levels, localization, or structure due to factors like modifications or interactions. The Orbitrap Astral Mass Spectrometer offers faster and more thorough analysis with heightened sensitivity. It generates high-quality data for accurate quantitation, boosting confidence in results. This is achieved through label-free quantitation (LFQ) or advanced methods like isobaric labeling with Tandem Mass Tags (TMT), ensuring meaningful outcomes in experiments.
"The quantitative results are pretty incredible. The Orbitrap Astral Mass Spectrometer dramatically increases the sensitivity of DIA and enables reproducible, single shot quantitation of over 4,500 proteins from enriched plasma with a 30-minute gradient."
— Mike MacCoss, Ph.D., Professor, Department of Genome Sciences, University of Washington
Hear from Mike MacCoss, Ph.D., Professor of Genome Sciences at the University of Washington, and Qing Yu, Research Associate at Harvard Medical School, as they discuss how the uniquely precise quantitation of the Orbitrap Astral Mass Spectrometer has benefitted their research.
Presentation by Mike MacCoss, Ph.D., Professor of Genome Sciences at the University of Washington
Presentation by Qing Yu, Research Associate at Harvard Medical School
Featured resources
Specification sheet
Rethink what is possible. Novel technology to advance your research capabilities. Learn what makes the Orbitrap Astral Mass Spectrometer different.
Brochure
The Orbitrap Astral Mass Spectrometer empowers new possibilities across an extensive set of applications. Learn more about some of these new capabilities.
White paper
The Orbitrap Astral Mass Spectrometer is ideally suited for accurate and precise quantitation at an unprecedented depth of coverage and throughput for samples from single cells to body fluids to bulk tissues.
Additional Resources
Style Sheet for Global Design System
Style Sheet for Komodo Tabs