Technical and educational videos for flow cytometry topics

flow cytometry educational videos and webinars

These videos, tutorials and webinars are designed to provide technical and education information regarding flow cytometers as well as flow cytometry research applications and techniques.

Learn more about the Invitrogen Attune Flow Cytometers

Learn more about the Invitrogen Bigfoot Spectral Cell Sorter


Featured videos

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Flow cytometry as you have never seen it before

This webinar addresses potential applications of integrating morphology analysis with standard flow cytometry, with specific focus on cancer research, such as those involving circulating tumor cells, cell mediated cytotoxicity, and apoptosis detection as you have never seen before.

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Exploring the five pillars of cell sorting: viability, recovery, efficiency, purity, and proliferation

Learn how to interpret post sort viability, recovery, efficiency, purity, and proliferation using the Bigfoot Spectral Cell Sorter.

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Identifying and sorting elusive cells using a high dimensional spectral panel

Using a 28-color mouse immunophenotyping panel we demonstrate the spectral unmixing and sorting capabilities of the Bigfoot Spectral Cell Sorter to resolve fluorescent signals accurately and precisely while sorting six ways simultaneously with high efficiency and purity.

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Benefits of combining morphometric data with flow cytometry: A panel discussion

A panel of experts discuss the benefits of imaging data in flow cytometry experiments, enabled with the Attune CytPix flow cytometer.

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Automated image analysis reduces user-to-user variability in flow cytometry gating strategies

This webinar describes how automated image analysis for morphometric parameters on the Attune CytPix flow cytometer enables reduced user-derived variability in flow cytometry experiments, enabling higher precision in your data.

Simplifying GxP Compliance from Acquisition to Final Result with the Attune Cytometric Software and FCS Express

Simplifying GxP Compliance from Acquisition to Final Result with the Attune Cytometric Software and FCS Express

With the release of 21 CFR Part 11 compliance ready software for Attune flow cytometers and De Novo Software’s continued support for compliance, scientists now have a complete end-to-end GxP flow cytometry acquisition and analysis.

Sample characterization of complex tissues on Attune CytPix Flow Cytometer

Sample characterization of complex tissues on Attune CytPix Flow Cytometer

Herve Luche of CIPHE presents data on characterization of complex tissues using the Attune CytPix Flow Cytometer.

Brightfield Imaging-enabled flow cytometry with the Attune CytPix

Brightfield Imaging-enabled flow cytometry with the Attune CytPix

Learn how the imaging-enhanced Attune CytPix flow cytometer is being used by the Amsterdam University Medical Centers Microscopy & Cytometry Core Facility to help users optimize their flow cytometry gating strategy and gain deeper insight into their sample. Using the imaging feature of the Attune CytPix, operators can quality control their flow cytometry samples and adjust gates to accurately exclude aggregates, coincident events, debris, and unwanted cells. Presented by Cora Chadick.

Profile photo of the presenter, R&D scientist Heaven Roberts

Integrating spatial resolution into flow cytometric analysis

Presented by R&D scientist Heaven Roberts, this webinar will further your understanding of how images can present novel data for cytometrists seeking even greater depth of insight into their samples and preparations— as well as how the Attune CytPix Flow Cytometer helps deliver enhanced imaging for your application.

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Easily upgrade your flow cytometry data with brightfield images using the Attune CytPix Flow Cytometer

Join Dr. Cora Chadick, as she demonstrates how easy it is to start collecting imaging flow cytometry data using the new Attune CytPix Flow Cytometer. Watch as she walks through the simple workflow, instrument setup, parameter optimization, data collection, and data analysis steps.

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Introducing the Attune CytPix Flow Cytometer: "Seeing is believing"

The Attune CytPix Flow Cytometer is an advanced cell analyzer that combines acoustic focusing fluidics for high sensitivity and high throughput with a high-speed camera.  Watch the CYTO 2021 presentation from product manager Kate Alford and lead engineer Greg Kaduchack.

Dr. Tim Inglis

Flow cytometry as a microbiology tool in the development of next generation diagnostics

Learn how Dr. Inglis and his team have harnessed the speed and precision of the Attune NxT to innovate a new generation of rapid microbiology research tools. They demonstrate how these tools function, how they can be used to accelerate laboratory workflows, and how the lessons learned can be applied to new and emerging challenges in the microbiology laboratory.

JordiPetriz

Endpoint assays for the characterization of rare and therapy-resistant cells in cancer

Learn from Jordi Petriz, from Josep Carreras Leukaemia Research Institute about using flow cytometry to detect and analyze immune cells using a no-wash no-lyse strategy.

Sarah DeBiasi

Infection with SARS-CoV-2 is associated with complex pathological conditions

One of these conditions observed in samples with severe SARS-CoV-2 infection is the progressive increase of immune-mediated inflammation, defined as a “cytokine storm.” Dr. Sara De Biasi describes the abnormally increased levels of cytokines in plasma from samples infected by SARS-CoV-2, and how flow cytometry has helped her investigate the immune system and cells producing cytokines in samples affected by coronavirus .

Justin Balko

Single-cell analyses of checkpoint-inhibition-induced myocarditis: implications into mechanisms of severe immune-related adverse events

Learn how Dr Justin Balko, Assoc Professor, Vanderbilt University, Department of Medicine, utilized flow cytometry to study immune checkpoint inhibitors as possible treatments in myocarditis associated with cancer.

Natasha Jacobsen

Learn how flow cytometry streamlines and enhances the CRISPR gene editing workflow

The advent of CRISPR has radically changed the face and pace of biological research and has unlocked unprecedented potential in the study of functional genomics, target identification, and gene therapy. Using flow cytometry in the gene editing workflow can both expedite and augment the process, while enabling a more in-depth and meaningful analysis of engineered cells.

Carol Oxford

Panel Design for Multi-Parameter Flow Cytometry

Join FAS Carol Oxford on panel design. Learn the value of including the correct controls and reagent titration. Understand the importance of fluorescence spillover and finding your fluorescence spectral balance.

Dr Prussak

The complex pharmacology of T-cell CARs

In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies.

CYToMORE 2016—Advances in cytometry

The 6th Modena Advanced Course in Cytometry (CYToMORE), was aimed at researchers who wanted to improve their flow cytometry knowledge and learn new applications. Organized in collaboration with the Italian Society of Immunology, Clinical Immunology and Allergology (SIICA) and led by teachers who are among the top international experts in the field, 2016 was a huge success. In this presentation, Victor Appay, Faculté de Médecine Pierre et Marie Curie, at Pitié-Salpêtrière, France discusses his work on “Assessing immunological aging using flow cytometry”.


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TypeTitle and descriptionCategories
CYTO 2022; webinar presentation Integrating spatial resolution into flow cytometric analysis
Presented by Heaven Roberts, R&D Scientist, Thermo Fisher Scientific
flow cytometry, imaging, cell analysis
CYTO 2021 poster presentation A Rapid Flow Virometric Test for Detection of SARS-CoV-2 Particles
Presented by Laura G. Rico, Josep Carreras Leukaemia Research Institute (IJC), ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Spain
flow cytometry, COVID-19, Coronavirus disease 2019
CYTO 2021 poster presentation Erythrolytic Buffers Have a Detrimental Impact in Flow Cytometric
Detrimental Impact in Flow Cytometric Dendritic Cell Detection Presented by Laura G. Rico, Josep Carreras Leukaemia Research Institute (IJC), ICO-Hospital Germans Trias i Pujol, Universitat Autònoma de Barcelona, Spain
flow cytometry, dendritic cell (DC)
CYToMORE lecture video (2016)...and on the 8th day, God created cytometry
Presented by J. Paul Robinson, Purdue University, USA
flow cytometers, flow cytometry, history, technology developments
CYToMORE lecture video (2016)Assessing immunological aging using flow cytometry
Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016)Asymptomatic but threatening: dissecting the consequences of cytomegalovirus infection
Presented by Anis Larbi, Singapore Immunology Network – SigN, Singapore
flow cytometry assays, immunology
CYToMORE lecture video (2016)Cancer stem cells and multi-drug resistance by flow cytometry
Presented by Jordi Petriz, Josep Carreras Leukaemia Research Institute, IJC, Spain
cancer, flow cytometry, multidrug resistance (MDR), stem cell research
CYToMORE lecture video (2016)Cytometric analysis of cytokines and transcription factors
Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany
cell signaling, flow cytometry, immunophenotyping
CYToMORE lecture video (2016)Cytometry of immunity
Presented by Andreas Radbruch, Deutsches Rheuma-Forschungszentrum Berlin, Germany
flow cytometry, immunology
CYToMORE lecture video (2016)Cytomic analysis of oxidative stress
Presented by J. Enrique O’Connor, University of Valencia, Spain
flow cytometry, oxidative stress
CYToMORE lecture video (2016)High throughput and high content flow cytometry
Presented by J. Paul Robinson, Purdue University, USA
Attune NxT, flow cytometer, high content flow cytometry, high throughput screening
CYToMORE lecture video (2016)Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy
Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy
flow cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016)Host-microbe interaction at mucosal surfaces
Presented by Maria Rescigno, Istituto Europeo di Oncologia – IEO, Italy
flow cytometry, immunology, microbiology
CYToMORE lecture video (2016)Mass cytometry in the phenomics era: an essential tool for gene/function studies
Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France
mass cytometry, immunology, immunophenotyping
CYToMORE lecture video (2016)Microenvironment cytometry
Presented by Paul Smith, Cardiff University, UK
cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery
CYToMORE lecture video (2016)Novel reagent solutions for flow cytometry
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometry assays
CYToMORE lecture video (2016)Rare cells analysis: from bench to clinics
Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy
Attune NxT, flow cytometer, rare cell detection, rare event detection
CYToMORE lecture video (2016)RNA flow cytometry
Presented by Paul Wallace, Roswell Park Cancer Institute, USA
flow cytometry, in situ hybridization, RNA
CYToMORE lecture video (2016)Software tutorial: what’s new
Presented by Carmen Gondhalekar, Purdue University, USA
Attune NxT, flow cytometer, software
CYToMORE lecture video (2016)Tuning acoustic forces for flow cytometry
Presented by Jolene Bradford, Thermo Fisher Scientific, USA
Attune NxT, flow cytometer
How-to videoTutorial: Labeling a purified antibody
Educational video on how to label your next antibody for imaging or fluorescence. Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment. The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes
How-to videoHow to site specifically label your antibody using SiteClick technology
This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach. This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology. Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection
Technology videoClick-iT Plus EdU Proliferation Assay
Measuring DNA synthesis is the most precise way to detect changes in cell proliferation. Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods. This video describes and compares two of the most referenced image-based proliferation technologies.
cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging
Technology videoThe Two Worlds of Cell Separationcell isolation, immunology
Tutorial videoMolecular Probes Tutorial Series—Analyzing Flow Cytometry Dataflow cytometer calibration, flow cytometry
Tutorial videoMolecular Probes Tutorial Series—Anatomy of Fluorescence Spectraabsorbance, emission, excitation, spectra
Tutorial videoMolecular Probes Tutorial Series—Introduction to Flow Cytometryflow cytometer calibration, multicolor flow cytometry
Tutorial videoMolecular Probes Tutorial Series—Introduction to Fluorescenceflow cytometry, fluorescence imaging
Tutorial videoMolecular Probes Tutorial Series—Overview of Filters and Light Sourcesemission, excitation, filters, light cubes, light sources
Video (Meet the Inventor)Attune NxT Flow Cytometer: Small in size, Big in Performance
Greg Kaduchak (Engineering Director Flow Cytometry, Thermo Fisher Scientific) gives an overview of the design and capabilities of the acoustic-assisted cytometry that's at the core Attune NxT Flow Cytometer.
Attune NxT
Video (Meet the Inventor)Attune NxT Flow Cytometer: Software
Jolene Bradford (Associate Director Flow Cytometry, Thermo Fisher Scientific) describes how the software of the Attune NxT Flow Cytometer helps users automate routine maintenance tasks, instrument performance alerts, and system cleaning.
Attune NxT
Video (Meet the Inventor)Attune NxT Flow Cytometer: Fluidics
Jolene Bradford (Associate Director Flow Cytometry, Thermo Fisher Scientific) speaks about how the fluidics system within the Attune NxT Flow Cytometer helps users minimize sample usage and avoid running buffer reservoirs dry.
Attune NxT
Video (Meet the Inventor)Attune NxT Flow Cytometer: Optics
Wesley Smith (Mechanical Engineer Flow Cytometry, Thermo Fisher Scientific) discusses the variety of laser configurations available on the Attune NxT Flow Cytometer, designed to support more advanced experiments and growing lab needs.
Attune NxT
Video (Meet the Inventor)Attune NxT Flow Cytometer: Acoustics
Michael Ward (Engineering Lead Flow Cytometry, Thermo Fisher Scientific) speaks about how the design of the Attune NxT Flow Cytometer offers smooth sample introduction, broadly variable volumetric flow rates, and robust performance for improved accuracy in cell analysis.
Attune NxT
Webinar Introducing flow cytometry-assisted antimicrobial susceptibility testing (FAST)
Learn how Dr. Tim Inglis, a clinical microbiologist at the University of Western Australia in Perth, leveraged the speed, accuracy, and broad drug/bug applicability of the Invitrogen Attune NxT Flow Cytometer to get same-day susceptibility results when combined with machine learning-based data analysis. Categories: flow cytometry, microbiology, flow cytometry-assisted antimicrobial susceptibility testing (FAST).
flow cytometry, microbiology, flow cytometry-assisted antimicrobial susceptibility testing (FAST)
Webinar The Role of Acoustic Cytometry in Cancer Immunotherapy and Leukemia
Learn from Jordi Petriz, PhD from Josep Carreras Leukaemia Research Institute about using flow cytometry to detect and analyze immune cells. In this video he discusses the use of flow cytometry to create the next generation of immunotherapy in hematological cancers.
flow cytometry, cancer, Attune NxT
Webinar Novel Fluorescent Assays and Labeling Strategies for Flow Cytometry
Learn about cell proliferation, apoptosis, and viability assays from Jolene Bradford
Flow cytometry, flow reagents
WebinarA practical approach to antibody labeling
The growing number of fluorophores available makes labeling your own antibodies a tempting proposition. But with many antibody labeling solutions available, selecting the best option can be a daunting task. In this webinar we will:
  • Provide an overview of our antibody labeling kits
  • Offer guidance on which methods are ideal for specific applications and experiments
  • Provide tips and tricks to optimize your labeling protocol
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF)
WebinarAn introduction to flow cytometric analysis, Part 1: Cell proliferation analysis
In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
WebinarAn introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis
In this webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability
WebinarAnalysis of surface antigens on exosomes using the Attune NxT Flow Cytometer
Join Steve McClellan as he shares how he analyzes individual exosomes for the expression of a variety of surface markers using multi-color flow cytometry.
Attune NxT, multicolor flow cytometry
WebinarBasics of flow cytometry, Part I: Gating and data analysis
An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry.
flow cytometry data, flow cytometry set up, multicolor flow cytometry
WebinarBasics of flow cytometry, Part II: Compensation
This presentation provides an overview of basic fluorochromes used in flow cytometry. Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation.
flow cytometry data, flow cytometry set up, multicolor flow cytometry
WebinarBasics of multi color flow cytometry panel design
With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever. This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
  • Rules for designing panels
  • Examples and practical application of these rules
  • Controls and standardization
  • Relevance of panel design to new mass cytometry platforms
antibodies, compensation, multicolor flow cytometry,
WebinarDNA content cell cycle analysis using flow cytometry
Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis. In this webinar we’ll discuss:
  • An overview of the methods and materials for using flow cytometry to determine cell cycle by measuring DNA content
  • Selection of DNA dyes for live cell and fixed cell analysis
  • Tips and tricks for consistent results
cell counting, cell cycle, cell proliferation, flow cytometry
WebinarFlow cytometry in microbiological research
In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments. During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods.
microbiology, multicolor flow cytometry, viability
WebinarHow recent technological advances in flow cytometry instrumentation are enabling faster throughput
This webinar presented by J. Paul Robinson at Purdue University focuses on advances in the Invitrogen Attune NxT Flow Cytometer and outline where the technology within the Attune NxT instrument fits in current and future applications.
Attune NxT, cell cycle, multicolor flow cytometry
WebinarLearn to choose the right fluorophore when designing experiments
The choice of fluorophore is one of the first important decisions to make in developing an experiment. Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength. Join our webinar and explore:
  • How to choose the best organic dye for an assay
  • Quantum dots and how they compare to other dyes
  • When to use a phycobiliprotein like R-PE or APC
  • When to use fluorescent proteins like GFP
  • How to choose a suitable dye to match your instrument
In addition, we will explore the basic characteristics, strengths, and weaknesses of the various fluorophores to help you choose and develop the best assay for your needs.
antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins
WebinarMaking polychromatic flow cytometry easy
Join Grace Chojnowski, Flow Cytometry and Imaging Facility Manager at QIMR Berghofer Medical Research Institute as she discusses how to make polychromatic flow cytometry easy after instrument standardization and verification for different fluorochromes on different flow cytometers and cell sorters.
Attune NxT, multicolor flow cytometry
WebinarMultiparameter cell cycle analysis
Join James W Jacobburger as he shares how to get better cell recovery and definition of cell cycle compartments. This webinar focuses on multiparametric cell cycle analysis of DNA and specific epitopes using a “washless” staining assay to minimize sample handling.
Attune NxT, multicolor flow cytometry
WebinarThe Complex Pharmacology of T-Cell CARs
In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies.
flow cytometry, immuno-oncology
WebinarPanel Design for Multi-Parameter Flow Cytometry
Join FAS Carol Oxford on panel design. Learn the value of including the correct controls and reagent titration. Understand the importance of fluorescence spillover and finding your fluorescence spectral balance.
multicolor flow cytometry, Attune NxT Flow Cytometer
WebinarIdentify Cancer Stem Cells in Primary Tumors and in Circulation using Flow Cytometry
Join Bruno Sainz as he discusses the advancements in the detection of cancer stem cells (CSC) in primary tumors and in circulation using acoustic flow cytometry.
flow cytometry
WebinarAnalyzing your CRISPR gene editing efficiencies using flow cytometry
In this webinar, Natasha Roark, scientist at Thermo Fisher Scientific, discusses CRISPR gene editing technology and its role in flow cytometry editing efficiencies.
flow cytometry
WebinarAdvancing immuno-oncology discovery and development with flow cytometry
In this webinar, Chris Langsdorf, scientist at Thermo Fisher Scientific, discusses how flow cytometry can effectively be employed in research efforts aimed at advancing the discovery and development of various immunotherapy strategies.
flow cytometry
Webinars (ongoing)Protein and cell analysis upcoming educational webinars
See our upcoming webinars and see what is now available on demand in our virtual learning platform.
education