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Real-time PCR master mix for microRNA analysis
MicroRNAs (miRNAs) are small, noncoding RNA molecules that direct post-transcriptional gene regulation. miRNA analysis requires reagents that are optimized to detect short microRNA targets expressed at low levels.
Applied Biosystems TaqMan Fast Advanced Master Mix has been optimized for use with our TaqMan Advanced miRNA assays to deliver highly sensitive detection of miRNA targets across a wide concentration range.
TaqMan Fast Advanced Master Mix
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Target |
cDNA (reverse transcribed from miRNA) |
Concentration |
2X |
Multiplexing/passive reference dye |
With ROX: up to 2 targets |
Cycling mode/run time |
Fast: ~40 min Standard: ~50 min |
Carryover contamination control |
Available in 2 versions: |
Reverse transcriptase |
Use TaqMan Advanced miRNA cDNA Synthesis Kit to perform the RT Step with TaqMan Advanced miRNA assays |
cDNA amplification (OPTIONAL) |
Use TaqMan PreAmp Master Mix to amplify small amounts of cDNA before performing qPCR |
Assay recommendation |
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For Research Use Only. Not for use in diagnostic procedures.