Live bovine pulmonary artery endothelial cells. MitoTracker Red CMXRos, anti–bovine COX subunit VIc, anti–human COX subunit I and Alexa Fluor 350 goat anti–mouse IgG antibodies.

Live bovine pulmonary artery endothelial cells (BPAEC) loaded with red-fluorescent MitoTracker Red CMXRos (M7512), fixed and permeabilized (middle). After fixation and permeabilization, the cells were treated with a cocktail containing two mouse monoclonal antibodies to cytochrome oxidase (the anti–OxPhos Complex IV subunit VIc (A6401) and anti–OxPhos Complex IV subunit I (A6403) antibodies), which were then detected using blue-fluorescent Alexa Fluor 350 goat anti–mouse IgG antibody (A11045) (left). The image in the right panel is an overlay of the first two images.

Live bovine pulmonary artery endothelial cells (BPAEC) loaded with red-fluorescent MitoTracker Red CMXRos (M7512), fixed and permeabilized (middle). After fixation and permeabilization, the cells were treated with a cocktail containing two mouse monoclonal antibodies to cytochrome oxidase (the anti–OxPhos Complex IV subunit VIc (A6401) and anti–OxPhos Complex IV subunit I (A6403) antibodies), which were then detected using blue-fluorescent Alexa Fluor 350 goat anti–mouse IgG antibody (A11045) (left). The image in the right panel is an overlay of the first two images.

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