Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody

Golgi and actin staining in HeLa cells. Golgi in fixed and permeabilized HeLa cells labeled with anti–golgin-97 monoclonal antibody (Cat. No. A21270) and visualized with green-fluorescent Alexa Fluor® 488 Goat-Anti–Mouse IgG (Cat. No. A11001). Actin was stained with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. No. A12381); nuclei were stained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). Treatment with Image-iT® FX signal enhancer (Cat. No. I36933) largely eliminates nonspecific dye binding (bottom) as compared to untreated slide (top).

Golgi and actin staining in HeLa cells. Golgi in fixed and permeabilized HeLa cells labeled with anti–golgin-97 monoclonal antibody (Cat. No. A21270) and visualized with green-fluorescent Alexa Fluor® 488 Goat-Anti–Mouse IgG (Cat. No. A11001). Actin was stained with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. No. A12381); nuclei were stained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). Treatment with Image-iT® FX signal enhancer (Cat. No. I36933) largely eliminates nonspecific dye binding (bottom) as compared to untreated slide (top).

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