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Unbiased label-free DIA quantitation
Benefit from uniquely accurate and precise quantitation, deep proteome coverage and data completeness. The premier quantitative Thermo Scientific Velocity LFQ data-independent acquisition (DIA) proteomics platform delivers high-confidence characterization of the differences between biological systems.
Now you can afford your first choice.The Velocity LFQ DIA platform includes the award-winning Thermo Scientific Orbitrap Exploris series of mass spectrometers, Thermo Scientific Vanquish Neo UHPLC system, custom Thermo Scientific μPAC Neo HPLC columns, Thermo Scientific EASY-Spray nano source, and the software you need to analyze your data.
Limited-time pricing and extended warranty
Learn about our limited-time value pricing and an extended warranty on the new Velocity LFQ data-independent acquisition (DIA) platform. Now is the time to get the accurate quantitation and precision you need, while managing your bottom line.
Download the Velocity DIA information kit to learn how to improve DIA performance on Orbitrap mass spectrometers.
This DIA infographic guides you through our DIA workflow, providing a step-by-step explanation. Utilize the interactive features to understand the functionality of each component within the workflow.
Increase protein identification and improve quantitative accuracy and precision by downloading our Velocity DIA technote specifically focused on the Orbitrap Exploris 480 mass spectrometer.
Infectious diseases diagnosis methods using LC-MS/MS and AI
High-throughput, high resolution DIA workflow for accurate label-free quantitation
Reproducible quantitative proteomics analysis using automated sample prep
Velocity LFQ DIA platform workflow
High-throughput high-resolution DIA workflow for accurate label-free quantitation
Velocity DIA workflow for label-free quantitation of proteome mixtures. Configuration highlights quantitative performance at high sample throughput, delivering robust and reproducible results. This workflow is a perfect fit for large-scale clinical and biomarker discovery studies.
The Velocity LFQ DIA platform is a premier quantitative proteomics system that uniquely delivers robust quantitative accuracy and precision, in combination with high proteome coverage and data completeness, for high-confidence, high-throughput characterization of large sample cohorts, for investigators in quantitative proteomics, translational research, biomarker discovery, and biopharma research labs, who need to characterize the differences between biological systems using precise quantitative measurements for hypothesis testing.
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Accurate and precise quantitation
- Determine correct protein abundance
- Reduces number of measurements needed to draw conclusions
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High confidence with deep proteome coverage and data completeness
- Direct DIA provides >99% data completeness
- Thermo Scientific Proteome Discoverer 3.1 software with CHIMERYS™ intelligent search algorithm by MSAID for deeper proteome profiling for screening approaches
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High throughput for large sample cohorts reduces time to biological insight
- Provides statistical significance
- Vanquish Neo UHPLC system and µPAC Neo columns provide high efficiency separations
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Robust platform and workflow solution
- Easy day-to-day platform operation for analyses of thousands of samples
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Wide dynamic range
- Discover differentially expressed low-abundance proteins of biological significance
- Confident quantitation of uncharacterized low-abundance proteins
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FAIMS Pro interface advantage
- Improves S/N to identify and quantify proteins from low sample volumes
- Increases spectral quality of MS/MS spectra, increasing confidence in identifications
"By performing high-throughput analyses on projects involving hundreds of clinical research samples, the DIA mode on the Orbitrap Exploris 480 mass spectrometer has enabled us to achieve deep and quantifiable proteome coverage (for Research Use Only) and reproducibility, maximizing the possibilities for biomarker discovery and validation."
Florence Roux-Dalvai, Proteomics project manager, ADLab, Research Center of CHU de Québec – Université Laval Hospital - Canada
Related products
Publications
Over one thousand peer-reviewed publications with Orbitrap mass spectrometers, covering a very broad range of proteomic applications, including for example analysis of SARS-CoV-2 host proteins, protein functions, noninvasive proteomics biomarkers, drugs, protein shapes and proteogenomic subtypes of acute myeloid leukemia. A subset of the 1000 publications are highlighted below.
Resources
On-demand Webinar: High Throughput Label-Free Quantitation with Orbitrap HR‑DIA Workflow
Unleashing the power of DIA acquisition on an Orbitrap Exploris 240 mass spectrometer – precise and accurate quantitation at 260 SPD
A Workflow for Highly Accurate Relative Label-Free Quantification of Microbial Proteins in Complex Cell Lysates
Velocity LFQ DIA workflow on an Orbitrap Exploris 480 mass spectrometer
LFQ HR-DIA Workflow for Plasma Proteomics on an Automated Platform
Velocity LFQ DIA workflow on an Orbitrap Ascend Tribrid mass spectrometer
Speak to an expert about the Velocity LFQ data-independent acquisition (DIA) platform
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