TaqMan Mutation Detection Assays detect and measure somatic mutations in genes that are associated with cancer. These research use only assays are powered by competitive allele-specific TaqMan PCR technology, also known as castPCR™ technology. The castPCR™ technology is highly specific and sensitive, and it can detect and quantitate rare amounts of mutated DNA in a sample that contains large amounts of normal, wild type DNA.

Experimental Workflow: Find the Products You Need for Every Step

Isolate and purify genomic DNA.

Products for Step 1: Prepare Sample

 

BloodPrep® DNA Chemistry for Cultured Cells and Blood
BloodPrep® DNA Chemistry is designed to rapidly isolate DNA from fresh or frozen blood from any animal species, tissue-cultured cells, or buccal swabs. The protocol is designed for use with the 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

Select appropriate TaqMan® Mutation Detection Assay products.

 

TaqMan® Mutation Detection Assays
Select from mutant allele, wild type allele, or gene reference assays.

Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® Real-Time PCR System or thermal cycler.

Reagents & Kits

 

TaqMan® Genotyping Master Mix
Obtain unrivaled cluster resolution for unambiguous SNP allelic discrimination. Optimized for end-point fluorescence detection in genotyping applications.

Instruments & Accessories

  
 

Veriti® 96-Well Thermal Cycler
The Veriti® 96-Well Thermal Cycler delivers excellent PCR and the proven reliability of Applied Biosystems 9600 and 9700 GeneAmp® PCR instruments. With the added control of VeriFlex™ Blocks, you have six independent temperature blocks providing precise control over your PCR optimization.

   
 

Dual 96-Well GeneAmp® PCR System 9700
GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The System consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with TaqMan® OpenArray® Genotyping Plates on the OpenArray Real-Time PCR System.

   
 

StepOnePlus™ Real-Time PCR System
Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

   
   
 

7500 Fast Real-Time PCR System
The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
   

 

 

 

 

7500 Real-Time PCR System
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

   
  

 

 

7900HT Fast Real-Time PCR System
The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

   
 

ViiA™ 7 Real-Time PCR System
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.    

Perform an endpoint plate read using Applied Biosystems® Real-Time PCR Systems.

Instruments & Accessories

  
   
 

StepOnePlus™ Real-Time PCR System
The StepOnePlus™ Real-Time PCR System makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

   
   
 

7500 Fast Real-Time PCR System
The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
   

 

 

 

  

 

 

7900HT Fast Real-Time PCR System
Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

   
 

ViiA™ 7 Real-Time PCR System
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.    

The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.

Mutation Detector™ Software
Mutation Detector™ Software helps determine the mutation status and percent mutation for  samples from data collected on supported Applied Biosystems® Real-Time PCR Systems.

Step 1 : Prepare Sample

Isolate and purify genomic DNA.

Products for Step 1: Prepare Sample

 

BloodPrep® DNA Chemistry for Cultured Cells and Blood
BloodPrep® DNA Chemistry is designed to rapidly isolate DNA from fresh or frozen blood from any animal species, tissue-cultured cells, or buccal swabs. The protocol is designed for use with the 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

Step 2 : Select Assays

Select appropriate TaqMan® Mutation Detection Assay products.

 

TaqMan® Mutation Detection Assays
Select from mutant allele, wild type allele, or gene reference assays.

Step 3 : Amplify DNA

Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® Real-Time PCR System or thermal cycler.

Reagents & Kits

 

TaqMan® Genotyping Master Mix
Obtain unrivaled cluster resolution for unambiguous SNP allelic discrimination. Optimized for end-point fluorescence detection in genotyping applications.

Instruments & Accessories

  
 

Veriti® 96-Well Thermal Cycler
The Veriti® 96-Well Thermal Cycler delivers excellent PCR and the proven reliability of Applied Biosystems 9600 and 9700 GeneAmp® PCR instruments. With the added control of VeriFlex™ Blocks, you have six independent temperature blocks providing precise control over your PCR optimization.

   
 

Dual 96-Well GeneAmp® PCR System 9700
GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The System consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with TaqMan® OpenArray® Genotyping Plates on the OpenArray Real-Time PCR System.

   
 

StepOnePlus™ Real-Time PCR System
Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

   
   
 

7500 Fast Real-Time PCR System
The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
   

 

 

 

 

7500 Real-Time PCR System
The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

   
  

 

 

7900HT Fast Real-Time PCR System
The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

   
 

ViiA™ 7 Real-Time PCR System
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.    

Step 4 : Allelic Discrimination

Perform an endpoint plate read using Applied Biosystems® Real-Time PCR Systems.

Instruments & Accessories

  
   
 

StepOnePlus™ Real-Time PCR System
The StepOnePlus™ Real-Time PCR System makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

   
   
 

7500 Fast Real-Time PCR System
The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
   

 

 

 

  

 

 

7900HT Fast Real-Time PCR System
Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

   
 

ViiA™ 7 Real-Time PCR System
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.    

Step 5 : Analyze Data

The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.

Mutation Detector™ Software
Mutation Detector™ Software helps determine the mutation status and percent mutation for  samples from data collected on supported Applied Biosystems® Real-Time PCR Systems.