Expand your molecular research offerings with infectious disease panels

 

A flexible, scalable, and reliable solution for lab optimization utilizing real-time PCR instrumentation

Expand your laboratory testing capabilities with PCR-based research solutions to accommodate a growing number of infectious disease areas, that enable early, accurate detection and differentiation compared to traditional methods. Detection of common infectious diseases with traditional culture- or microscopy-based methods are antiquated and lack sensitivity and specificity. Real-time PCR (qPCR) is an innovative solution for detecting slow-growing, difficult-to-cultivate, or uncultivable microorganisms.

In order to effectively study outbreaks and determine disease etiology where pathogens are similar, analytically sensitive panels are needed to support laboratory research studies.

Applied Biosystems TrueMark Infectious Disease Research Panels are convenient, duplexed TaqMan assays that are performed on pre-spotted and dried-down plates that include microorganism‑specific pathogens, utilizing real‑time PCR techniques.

This enables rapid, accurate detection and categorization of microorganisms that cause respiratory, vaginal, urinary, gastrointestinal, wound, and sexually transmitted diseases, as well as antibiotic resistance research.

Our portfolio of infectious disease panels are available in predefined and customizable options. Choose from an extensive list of pathogens to create your own plate, or ten different predefined panels in several disease states, with preselected assay targets.


Key features


Benefits of the TrueMark Predefined Plus and Custom Panels
 

Internal process controls: RNase P human internal control or Bacillus atrophaeus bacterial spike-in control


Additional benefits of TrueMark Predefined Plus and Custom Panels

Duplexed assays 

  • Allow for the simultaneous detection of target and control in the same well
  • Help increase confidence in results
  • Are designed to reduce the need to run replicates, helping to lower cost and minimize workflow complexity

Pre-spotted and dried down plates

  • Target and RNAse P or Bacillus atrophaeus* process control assays dried down in plate 
  • Designed to reduce hands on time and errors in the workflow

Amplification control

  • One control covers all disease area targets, panels, and applications
  • Makes it easier and faster for labs to implement

For Research Use Only. Not for use in diagnostic procedures.

 

Maximize your workflow

Expand your research testing menu with the TrueMark Infectious Disease Research Panels utilizing one workflow, with our custom, made-to-order, or predefined panels on TaqMan Array Plates for 96-well and 384-well panels which are compatible for use on QuantStudio qPCR systems. Go from sample to result in approximately four hours.

New to Thermo Fisher molecular testing?  Easily click on the steps to below to see the recommended components needed to set up your laboratory for real-time PCR testing.


  • Vaginal swab**, genital swab, lesion swab, nasopharyngeal swab**, nasopharyngeal aspirate**, or urine sample**

 

Use existing workflow including KingFisher systems and MagMAX kits

  • KingFisher Flex Purification System
  • KingFisher Apex Purification System
  • Applied Biosystems MagMAX Ultra
    Nucleic Acid Isolation Kits
  • TrueMark Comprehensive Microbiota Control

  • Select panel for either 96- or 384-well block plate, and add sample and Applied Biosystems multiplex master into wells
  • Applied Biosystems QuantStudio 5 Real
    -Time PCR System
  • Applied Biosystems QuantStudio 7 Flex
    Real-Time PCR System
  • Applied Biosystems QuantStudio 7 Pro Real-
    Time PCR System
  • Applied Biosystems QuantStudio 12K Flex
    Real-Time PCR System
  • Applied Biosystems QuantStudio 5 Dx Real -Time PCR System
  • Applied Biosystems QuantStudio 7 Pro Dx Real -Time PCR System

 QuantStudio 5 Dx and QuantStudio 7 Pro Dx systems are For In Vitro Diagnostic Use. Test development mode is for Research Use Only. Not for use in diagnostic procedures.

  • Applied Biosystems QuantStudio Design and Analysis Software


  • Vaginal swab**, genital swab, lesion swab, nasopharyngeal swab**, nasopharyngeal aspirate**, or urine sample**

 

Use existing workflow including KingFisher systems and MagMAX kits

  • KingFisher Flex Purification System
  • KingFisher Apex Purification System
  • Applied Biosystems MagMAX Ultra
    Nucleic Acid Isolation Kits
  • TrueMark Comprehensive Microbiota Control

  • Select panel for either 96- or 384-well block plate, and add sample and Applied Biosystems multiplex master into wells
  • Applied Biosystems QuantStudio 5 Real
    -Time PCR System
  • Applied Biosystems QuantStudio 7 Flex
    Real-Time PCR System
  • Applied Biosystems QuantStudio 7 Pro Real-
    Time PCR System
  • Applied Biosystems QuantStudio 12K Flex
    Real-Time PCR System
  • Applied Biosystems QuantStudio 5 Dx Real -Time PCR System
  • Applied Biosystems QuantStudio 7 Pro Dx Real -Time PCR System

 QuantStudio 5 Dx and QuantStudio 7 Pro Dx systems are For In Vitro Diagnostic Use. Test development mode is for Research Use Only. Not for use in diagnostic procedures.

  • Applied Biosystems QuantStudio Design and Analysis Software

Analytical validation consultation services for research products

When adopting a new testing method for your research needs, we can assist in enabling the verification of the workflow to help ensure a successful launch. Our analytical validation (AV) consulting services can speed up the launch process by up to 75% and cut costs by up to half. Our expert team will consult to help develop and optimize your research workflow, while providing data analysis support and template documentation to fully maximize your instrument and reagent investment.*

* Consulting services offered for enabling the analytical validation cannot be used to validate a clinical diagnostic workflow and can only be used to support ongoing true research efforts.


TrueMark Predefined Plus Panel options

Choose from ten different predefined panels in several disease areas, covering 8 pathogens each. Pathogens included in predefined options are to be used for research purposes only.

Table 1. Applied Biosystems TrueMark Respiratory I Plus Panel

Pathogen TypePathogensSample Type

Viral

Adenovirus
Enterovirus
Influenza A (Pan) Influenza B
Respiratory Syncytial Virus A/B (RSV A/B)
Rhinovirus
Human Metapneumovirus (HM)

SARS-CoV-2 ORF1ab

 

 

Nasopharyngeal swab** or

nasopharyngeal aspirate**

Control Type

RNase P internal control

Table 2. Applied Biosystems TrueMark Respiratory II Plus Panel

Pathogen TypePathogensSample Type

Viral

Coronavirus 229E (CE)
Coronavirus HKU1 (CH)
Coronavirus NL63 (CN)
Coronavirus OC43 (CO)
Parainfluenza 1 (P1)
Parainfluenza 2 (P2)
Parainfluenza 3 (P3)
Parainfluenza 4 (P4)

 

 

Nasopharyngeal swab** or

nasopharyngeal aspirate**

Control Type

RNase P internal control

Table 3. Applied Biosystems TrueMark Respiratory III Plus Panel

Pathogen TypePathogensSample Type

Bacterial

C. pneumoniae
Moraxella catarrhalis
Klebsiella pneumonia
Staphylococcus aureus (SA)
Haemophilus Influenzae (HI)
Legionella pneumophila (LP)
Mycoplasma pneumoniae (MP)
Streptococcus pneumoniae (SP)

 

 

Nasopharyngeal swab** or

nasopharyngeal aspirate**

Control Type

RNase P internal control

 

Table 4. Applied Biosystems TrueMark Urinary I Plus Panel

Pathogen TypePathogensSample Type

Bacterial

Pseudomonas aeruginosa
Proteus mirabilis
Escherichia coli
Klebsiella pneumoniae
Staphylococcus aureus
Coagulase negative staphylococci
Staphylococcus saprophyticus

 

 

 

Urine sample**

Fungal

Candida albicans

Control Type

Bacillus Atrophaeus spike-in control*

 

Table 5. Applied Biosystems TrueMark Urinary II Plus Panel

Pathogen TypePathogensSample Type

Bacterial

Corynebacterium riegelii
Enterobacter aerogenes
Aerococcus urinae
Proteus vulgaris
Acinetobacter baumannii
Actinobaculum schaalii
Enterococcus faecalis
Streptococcus agalactiae

 

 

Urine sample**

Control Type

Bacillus Atrophaeus spike-in control*

Table 6. Applied Biosystems TrueMark Urinary III Plus Panel

Pathogen TypePathogensSample Type

Bacterial

Citrobacter freundii
Enterobacter cloacae
Enterococcus faecium
Klebsiella oxytoca
Serratia marcescens
Mycoplasma hominis
Morganella morganii
Providencia stuartii

 

 

Urine sample**

Control Type

Bacillus Atrophaeus spike-in control*

Table 7. Applied Biosystems TrueMark STI Plus Panel

Pathogen TypePathogensSample Type

Bacterial

Chlamydia trachomatis (CT)
Neisseria gonorrhoeae (NG)
Mycoplasma genitalium (MG)
Mycoplasma hominis (MH)
Ureaplasma parvum (UP)
Ureaplasma urealyticum (UU)
Herpes simplex virus type 1 & 2 (HSV1 + HSV2)

 

 

Vaginal swab** or

genital swab

Parasite

Trichomonas vaginalis (TV)

Control Type

RNase P internal control

Table 8. Applied Biosystems TrueMark Vaginal Plus Panel

Pathogen TypePathogensSample Type

Bacterial

Gardnerella vaginalis (GV)
Atopobium vaginae (AV)
Prevotella bivia
Mobiluncus curtisii (MC)
Bacteroides fragilis
Pan–Lactobacillus
Megasphaera Type 1 (Mega1)
Bacterial vaginosis–associated bacteria 2 (BVAB2)

 

 

Vaginal swab**

Control Type

RNase P internal control

Table 9. Applied Biosystems TrueMark Lesion Plus Panel

Pathogen TypePathogensSample Type

Bacterial

Haemophilus ducreyi (HD)
Treponema pallidum (TP)
Chlamydia trachomatis L1, L2, and L3 (lymphogranuloma venereum)

 

 

 

Lesion swab

Viral

Herpes simplex virus type 1 (HSV1)
Herpes simplex virus type 2 (HSV2)
Cytomegalovirus (CMV)
Varicella-zoster virus (VZV)

Fungal

Pan-Candida

Control Type

RNase P internal control

Table 10. Applied Biosystems TrueMark Genital Plus Panel

Pathogen TypePathogensSample Type

Bacterial

Gardnerella vaginalis (GV)

 

 

 

Vaginal swab** or

genital swab

Fungal

Candida albicans
Candida krusei
Candida dubliniensis
Candida glabrata
Candida tropicalis
Candida parapsilosis

Parasite

Trichomonas vaginalis (TV)

Control Type

RNase P internal control


Easy-to-Build Custom Infectious Disease Research Panels***

There is also the option to easily select and build custom infectious disease research panels, from 4 to 24 targets, in various preferred research formats, providing complete flexibility to meet the needs of each laboratory.

Custom options are available on 96-well- or 384-well plates.

How to build a custom panel workflow image

Custom assay design

Ready to start building your custom panel? We offer an extensive list of microbe assays and antibiotic resistance assays in several disease areas to choose from that provide options for flexibility to customize panels that fit your laboratory's needs and priorities. Please browse through our portfolio of microbe assays and resistance gene assays.


Benefits of molecular testing
 

Increased sensitivity: more specific, accurate and precise compared to traditional culture and microscopy methods
Timely results: results in as little as 4-6 hours


Benefits of buying from Thermo Fisher Scientific  

 


Ordering information


TrueMark Infectious Disease Predefined Plus Panel offerings

Combo Kit components †

Catalog #

Name

Size

Included in Combo Kit †

TrueMark Infectious Disease 1-Step Multiplex Master Mix (No ROX)

3 X 1 mL

 

TrueMark Infectious Disease Custom Panel offerings

Custom Plate Options ***

Description

Cat. No. ***

TrueMark Respiratory Panel

Contact sales representative ›

TrueMark Urinary Panel

Contact sales representative ›

TrueMark STI and Vaginal Panels

Contact sales representative ›

TrueMark Gastrointestinal Panel

Contact sales representative ›

TrueMark Antibiotic Resistance Panel

Contact sales representative ›

TrueMark Custom Assay Panel

Contact sales representative ›

 

For use with TrueMark Infectious Disease Custom Panels, sold separately

Additional components needs for both TrueMark Infectious Disease Predefined and Custom Panels, sold separately


For Research Use Only. Not for use in diagnostic procedures.

References

1. Finegold, David N. “Genetic Diagnostic Technologies - Fundamentals.” Merck Manuals Consumer Version, Merck Manuals, 21 Feb. 2023, https://www.merckmanuals.com/home/fundamentals/genetics/genetic-diagnostic-technologies.

2. Vazquez-Pertejo, Maria T. “Diagnosis of Infectious Disease - Infections.” Merck Manuals Consumer Version, Merck Manuals, 21 Feb. 2023, https://www.merckmanuals.com/home/infections/diagnosis-of-infectious-disease/diagnosis-of-infectious-disease.

Footnotes

*Spike in the Applied Biosystems TaqMan Universal Extraction Control Organism (Cat. No. A39180) containing Bacillus atrophaeus for use as a process control.

**Internally tested sample types.

***For specific panel options that are available with each Cat. No., contact your sales representative.

†Combo Kits include 5 TaqMan Array plates and 3 x 1 mL tubes of TrueMark Infectious Disease Multiplex Master Mix (No ROX)

For Research Use Only. Not for use in diagnostic procedures.

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