TaqMan SARS-CoV-2 2.0 Assays | Thermo Fisher Scientific

Evolved for choice and longevity—TaqMan SARS-CoV-2 2.0 assays

Choose from our two new advanced assays of Applied Biosystems TaqMan SARS-CoV-2 2.0 assays that deliver the same accuracy and reliability that our SARS-CoV-2 assays are known for, while offering the ability to accommodate multiple sample types and the choice to utilize either a standard or fast workflow. The standard workflow includes RNA extraction and traditional sample preparation, and the fast workflow is a fast, direct-to-PCR workflow with no RNA extraction required. You’ll also have confidence in your results knowing that our 2.0 assays include enhanced target redundancy to compensate for all SARS-CoV-2 mutations known now and those yet to be discovered in the future.

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Features of the TaqMan SARS-CoV-2 2.0 assays include:

Same accuracy and reliability you have grown to trust—an advanced assay design using multiple targets on orf1a, orf1b, and N genes (Figure 1) compensates for current and emerging SARS-CoV-2 mutations, helping provide continued confidence in results now and into the future
Multiple workflow options—choose from two assays: one utilizes a standard workflow that includes RNA extraction (Figure 2), and the other utilizes a fast, direct-to-PCR workflow with no RNA extraction required (Figure 3)
Affordable and scalable—add to your existing SARS-CoV-2 menu using your current real-time PCR instrumentation

Product details for TaqMan 2.0 assays:

	TaqMan SARS-CoV-2 Fast PCR Combo Kit 2.0	TaqMan SARS-CoV-2 RNase P Assay 2.0
Description	Fast, direct-to-PCR, raw saliva solution using an evolved assay design to enable widespread, high-frequency testing (Figure 3)	An evolved SARS-CoV-2 assay design, with RNase P for human sample confirmation (Figure 2)
Assay design	3 genomic regions (orf1a, orf1b, and N genes) with 8 total targets (Figure 1)
Internal control	RNase P	RNase P
Compatible sample types	Raw saliva collected with: SpeciMAX raw saliva collection kit Leak-proof, screw top tubes Other conical tubes	RNA extracted from nasopharyngeal swabs and anterior nasal swabs
Recommended sample preparation	Lysis buffer (SalivaReady Solution, part of the TaqMan SARS-CoV-2 Fast PCR Combo Kit 2.0)	KingFisher Flex Purification System with MagMAX Viral/Pathogen II Nucleic Acid Isolation Mix
Recommended real-time PCR instruments	QuantStudio 5 Real-Time PCR system (96-well, 0.2 mL block) QuantStudio 7 Flex Real-Time PCR system (384-well block)
Turnaround time (including sample prep)	~ 2 hours	~ 3 hours
Recommended software	QuantStudio Design & Analysis Software, RUO Edition 2.5 or higher