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The Gibco AAV-MAX Helper-Free AAV Production System is an adeno-associated virus (AAV) production system developed for scalable, high-titer AAV production. The AAV-MAX system includes a HEK293F-derived clonal suspension cell line adapted to a chemically defined medium; transfection reagent; transfection booster; and a production enhancer. All components of the system are animal origin-free.