Attune CytPix

The Invitrogen Attune cytometers can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry, to match the panels you are currently running. Multiple fluorescent proteins can be interrogated with the 4-laser version of the Attune Flow Cytometers.

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Download or Print a poster-sized reagent selection guide

Find reagents for flow cytometrySelect fluorophoreFind antibodies

For a reagent guide for the first-generation Attune flow cytometer, see Resources for the Original Attune Cytometer


Find compatible reagents by excitation source

Each of the tabs below shows the antibody labels and application-based dyes and fluorescent reagents that are compatible with a given laser excitation source. Green laser configuration not available on the Attune CytPix model.

Violet 6 laser (405 nm) reagents

 BlueGreenOrangeRedFar RedNear IR
Emission filter450/40 nm525/50 nm610/20 nm660/20 nm710/50 nm780/60 nm
Channel nameVL1VL2VL3VL4VL5VL6
Compatible fluorescent proteins▪ Azurite
▪ Cerulean
▪ eBFP
▪ eCFP
▪ mTurquoise
▪ Sirius
▪ T-Sapphire
▪ CFP
▪ vGFP
    
Antibody labels▪ Super Bright 436
▪ eFluor 450
▪ VioBlue
▪ Brilliant Violet 421
▪ Pacific Blue
▪ BD Horizon V450
▪ eFluor 506
▪ VioGreen
▪ Brilliant Violet 510
▪ Pacific Green
▪ BD Horizon V500
▪ Super Bright 600
▪ Qdot 605
▪ Pacific Orange
▪ Brilliant Violet 605
▪ Super Bright 645
▪ Brilliant Violet 650
▪ Super Bright 702
▪ Brilliant Violet 711
▪ Qdot 705
▪ Super Bright 786
▪ Brilliant Violet 786
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Violet▪ LIVE/DEAD Fixable Aqua▪ LIVE/DEAD Fixable Yellow   
Viability dyes (non-fixed cells)▪ DAPI
▪ SYTOX Blue
     
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Violet     
DNA content/
cell cycle dyes (fixed cells)
▪ FxCycle Violet     
Apoptosis dyes▪ Annexin V AF350
▪ PO-PRO-1
▪ Violet Ratiometric Probe (F2N12S)▪ Violet Ratiometric Probe (F2N12S)   
Cell proliferation▪ Click-iT Plus Edu Pacific Blue
▪ CellTrace Violet
     
ROS detection      
Phagocytosis      

Violet laser (405 nm) reagents

 BlueGreenOrangeRed
Emission filter440/50 nm512/25 nm603/48 nm710/50 nm
Channel nameVL1VL2VL3VL4
Compatible fluorescent proteins▪ Sirius
▪ Azurite
▪ eCFP
▪ eBFP
▪ mTurquoise
▪ Cerulean
▪ vGFP  
Antibody labels▪ Super Bright 436
▪ Alexa Fluor 405
▪ Pacific Blue
▪ Pacific Green
▪ Qdot 525
▪ Super Bright 600
▪ Pacific Orange
▪ Qdot 605
▪ Super Bright 645
▪ Qdot 705
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Violet▪ LIVE/DEAD Fixable Aqua▪ LIVE/DEAD Fixable Yellow 
Viability dyes (non-fixed cells)▪ DAPI
▪ SYTOX Blue
 ▪ PI 
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Violet   
DNA content/
cell cycle dyes (fixed cells)
▪ FxCycle Violet   
Apoptosis dyes▪ Annexin V AF350
▪ PO-PRO-1
▪ Violet Ratiometric Probe (F2N12S)▪ Violet Ratiometric Probe (F2N12S) 
Cell proliferation▪ Click-iT Plus Edu Pacific Blue
▪ CellTrace Violet
   
ROS detection    
Phagocytosis    

Blue laser (488 nm) reagents

ColorGreenOrangeOrangeFar RedNear IR
Emission filter530/30 nm574/26 nm590/40 nm695/40 nm780/60 nm
ChannelBL1BL2
(without yellow laser present)
BL2
(with yellow laser present)
BL3BL4
Compatible fluorescent proteins

▪ eGFP
▪ Emerald
▪ eYFP

▪ eYFP
▪ mCitrine
▪ Venus
   
Antibody labels▪ Alexa Fluor 488
▪ FITC
▪ Alexa Fluor 546
▪ Qdot 565
▪ Qdot 585
▪ PE
▪ PE-Alexa Fluor 610
▪ PE-Texas Red
▪ Qdot 585
▪ PE
▪ PE-Alexa Fluor 700
▪ PE-Cy5.5
▪ PerCP
▪ PerCP-Cy5.5
▪ Qdot 705
▪ Tri-Color
▪ Pe-Cy7
▪ PE-Alexa Fluor 750
▪ Qdot 800
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Green ▪ LIVE/DEAD Fixable Red  
Viability dyes (non-fixed cells)▪ SYTOX Green▪ PI
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX Orange
▪ SYTOX AADVanced
▪ 7-AAD
▪ PI
▪ SYTOX AADVanced
 
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Green
▪ Vybrant DyeCycle Orange  ▪ Vybrant DyeCycle Ruby
DNA content/
cell cycle dyes (fixed cells)
  ▪ FxCycle PI/Rnase  
Apoptosis dyes▪ Annexin V AF488
▪ MitoProbe JC-1
▪ CellEvent Caspase-3/7 Green
▪ anti-PARP-FITC
▪ YO-PRO-1 iodide
▪ APO-BrdU TUNEL - Alexa Fluor 488
▪ MitoProbe Transition Pore
▪ MitoProbe DiOC2(3)
▪ Annexin V FITC
▪ Annexin V PE
▪ TMRE
▪ TMRM
▪ MitoProbe JC-1
▪ Annexin V PE
▪ TMRE
▪ TMRM
  
Cell proliferation▪ Click-iT Plus Edu Alexa Fluor 488
▪ CellTrace CFSE
    
ROS detectionCellROX Green    
Phagocytosis▪ pHrodo Green E. coli BioParticles
▪ pHrodo Green S. aureus BioParticles
▪ pHrodo Red E. coli BioParticles
▪ pHrodo Red Phagocytosis
   

Green laser (532 nm) reagents

ColorOrangeRedFar RedNear IR
Emission filter575/36 nm620/15 nm695/40 nm780/60 nm
ChannelGL2GL2GL3GL4
Compatible fluorescent proteins▪ tdTomato
▪ YFP
▪ tmCherry  
Antibody labels

▪ Alexa Fluor 532 ▪ Alexa Fluor 555
▪ DsRed
▪ Qdot 565
▪ Qdot 605
▪ R-phycoerythrin (R-PE, PE)

▪ Alexa Fluor 568
▪ Alexa Fluor 594
▪ Qdot 605
▪ PE-Texas Red

▪ Qdot 705
▪ PE-Alexa Fluor 700
▪ TRI-COLOR (PE-Cy5)
▪ PE-Cy5.5

▪ Qdot 800
▪ PE-Cy7

Viability dyes (compatible with fixation) ▪ LIVE/DEAD Fixable Red  
Viability dyes (non-fixed cells)▪ SYTOX Orange▪ SYTOX AADVanced
▪ PI
▪ SYTOX AADVanced 
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Orange▪ Vybrant DyeCycle Orange▪ Vybrant DyeCycle Ruby 
DNA content/
cell cycle dyes (fixed cells)
 ▪ FxCycle PI/Rnase  
Apoptosis dyes▪ Annexin V, R-PE
▪ Annexin V, Alexa Fluor 555
▪ MitoTracker Orange CMTMRos
▪ Annexin V, Alexa Fluor 568
▪ Annexin V, Alexa Fluor 594
▪ MitoTracker Red MNXRos
  
Cell proliferation▪ CellTrace Yellow

▪ Click-iT Plus EDU
▪ Alexa Fluor 594

  
ROS detection▪ CellROX Orange   
Phagocytosis▪ pHrodo Red E. coli BioParticles
   

Yellow laser (561 nm) reagents

ColorOrangeRedFar redNear IR
Emission filter585/16 nm620/15 nm695/40 nm780/60 nm
ChannelYL1YL2YL3YL4
Compatible fluorescent proteins▪ mOrange
▪ RFP
▪ dTomato
▪ mCherry
▪ DsRed
▪ mKate
▪ mStrawberry
  
Antibody labels▪ Alexa Fluor 568
▪ PE
▪ Qdot 565
▪ Qdot 585
▪ Alexa Fluor 594
▪ PE- Alexa Fluor 610
▪ PE-Texas Red
▪ PE-Alexa Fluor 700
▪ PE-Cy5.5
▪ Qdot 705
▪ TRI-COLOR
▪ PE-Alexa Fluor 750
▪ PE-Cy7
▪ Qdot 800
Viability dyes (compatible with fixation)    
Viability dyes (non-fixed cells)▪ PI
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX AADvanced
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX AADvanced
 
DNA content/cell cycle dyes (live cells)▪ Vybrant DyeCycle Orange  ▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells) ▪ FxCycle PI/Rnase  
Apoptosis dyes▪ Annexin V AF568
▪ Annexin V AF594
▪ MitoTracker Red CMXRos
▪ MitoTracker Orange CMTMRos
▪ TMRM
   
Cell proliferation    
ROS detection▪ CellROX Orange   
Phagocytosis    

Red (637 nm) laser reagents

ColorFar RedFar RedNear IR
Emission filter670/14 nm720/30 nm780/60 nm
ChannelRL1RL2RL3
Compatible fluorescent proteins   
Antibody labels▪ APC
▪ Alexa Fluor 647
▪ Alexa Fluor 660
▪ Qdot 655
▪ Alexa Fluor 680
▪ Alexa Fluor 700
▪ APC-Alexa Fluor 700
▪ Qdot 705
▪ APC-Alexa Fluor 750
▪ APC-Cy7
▪ Qdot 800
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Far Red ▪ LIVE/DEAD Fixable Near-IR
Viability dyes (non-fixed cells)▪ SYTOX Red  
DNA content/cell cycle dyes (live cells)  ▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells)▪ FxCycle Far Red  
Apoptosis dyes▪ Annexin V APC
▪ Annexin V AF647
▪ Annexin V AF 680
▪ TO-PRO-3
▪ MitoProbe DilC1(5)
  
Cell proliferation▪ Click-iT Plus Edu Alexa Fluor 647
▪ CellTrace Far Red
  
ROS detection▪ CellROX Deep Red  
Phagocytosis   

Violet 6 laser (405 nm) reagents

 BlueGreenOrangeRedFar RedNear IR
Emission filter450/40 nm525/50 nm610/20 nm660/20 nm710/50 nm780/60 nm
Channel nameVL1VL2VL3VL4VL5VL6
Compatible fluorescent proteins▪ Azurite
▪ Cerulean
▪ eBFP
▪ eCFP
▪ mTurquoise
▪ Sirius
▪ T-Sapphire
▪ CFP
▪ vGFP
    
Antibody labels▪ Super Bright 436
▪ eFluor 450
▪ VioBlue
▪ Brilliant Violet 421
▪ Pacific Blue
▪ BD Horizon V450
▪ eFluor 506
▪ VioGreen
▪ Brilliant Violet 510
▪ Pacific Green
▪ BD Horizon V500
▪ Super Bright 600
▪ Qdot 605
▪ Pacific Orange
▪ Brilliant Violet 605
▪ Super Bright 645
▪ Brilliant Violet 650
▪ Super Bright 702
▪ Brilliant Violet 711
▪ Qdot 705
▪ Super Bright 786
▪ Brilliant Violet 786
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Violet▪ LIVE/DEAD Fixable Aqua▪ LIVE/DEAD Fixable Yellow   
Viability dyes (non-fixed cells)▪ DAPI
▪ SYTOX Blue
     
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Violet     
DNA content/
cell cycle dyes (fixed cells)
▪ FxCycle Violet     
Apoptosis dyes▪ Annexin V AF350
▪ PO-PRO-1
▪ Violet Ratiometric Probe (F2N12S)▪ Violet Ratiometric Probe (F2N12S)   
Cell proliferation▪ Click-iT Plus Edu Pacific Blue
▪ CellTrace Violet
     
ROS detection      
Phagocytosis      

Violet laser (405 nm) reagents

 BlueGreenOrangeRed
Emission filter440/50 nm512/25 nm603/48 nm710/50 nm
Channel nameVL1VL2VL3VL4
Compatible fluorescent proteins▪ Sirius
▪ Azurite
▪ eCFP
▪ eBFP
▪ mTurquoise
▪ Cerulean
▪ vGFP  
Antibody labels▪ Super Bright 436
▪ Alexa Fluor 405
▪ Pacific Blue
▪ Pacific Green
▪ Qdot 525
▪ Super Bright 600
▪ Pacific Orange
▪ Qdot 605
▪ Super Bright 645
▪ Qdot 705
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Violet▪ LIVE/DEAD Fixable Aqua▪ LIVE/DEAD Fixable Yellow 
Viability dyes (non-fixed cells)▪ DAPI
▪ SYTOX Blue
 ▪ PI 
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Violet   
DNA content/
cell cycle dyes (fixed cells)
▪ FxCycle Violet   
Apoptosis dyes▪ Annexin V AF350
▪ PO-PRO-1
▪ Violet Ratiometric Probe (F2N12S)▪ Violet Ratiometric Probe (F2N12S) 
Cell proliferation▪ Click-iT Plus Edu Pacific Blue
▪ CellTrace Violet
   
ROS detection    
Phagocytosis    

Blue laser (488 nm) reagents

ColorGreenOrangeOrangeFar RedNear IR
Emission filter530/30 nm574/26 nm590/40 nm695/40 nm780/60 nm
ChannelBL1BL2
(without yellow laser present)
BL2
(with yellow laser present)
BL3BL4
Compatible fluorescent proteins

▪ eGFP
▪ Emerald
▪ eYFP

▪ eYFP
▪ mCitrine
▪ Venus
   
Antibody labels▪ Alexa Fluor 488
▪ FITC
▪ Alexa Fluor 546
▪ Qdot 565
▪ Qdot 585
▪ PE
▪ PE-Alexa Fluor 610
▪ PE-Texas Red
▪ Qdot 585
▪ PE
▪ PE-Alexa Fluor 700
▪ PE-Cy5.5
▪ PerCP
▪ PerCP-Cy5.5
▪ Qdot 705
▪ Tri-Color
▪ Pe-Cy7
▪ PE-Alexa Fluor 750
▪ Qdot 800
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Green ▪ LIVE/DEAD Fixable Red  
Viability dyes (non-fixed cells)▪ SYTOX Green▪ PI
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX Orange
▪ SYTOX AADVanced
▪ 7-AAD
▪ PI
▪ SYTOX AADVanced
 
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Green
▪ Vybrant DyeCycle Orange  ▪ Vybrant DyeCycle Ruby
DNA content/
cell cycle dyes (fixed cells)
  ▪ FxCycle PI/Rnase  
Apoptosis dyes▪ Annexin V AF488
▪ MitoProbe JC-1
▪ CellEvent Caspase-3/7 Green
▪ anti-PARP-FITC
▪ YO-PRO-1 iodide
▪ APO-BrdU TUNEL - Alexa Fluor 488
▪ MitoProbe Transition Pore
▪ MitoProbe DiOC2(3)
▪ Annexin V FITC
▪ Annexin V PE
▪ TMRE
▪ TMRM
▪ MitoProbe JC-1
▪ Annexin V PE
▪ TMRE
▪ TMRM
  
Cell proliferation▪ Click-iT Plus Edu Alexa Fluor 488
▪ CellTrace CFSE
    
ROS detectionCellROX Green    
Phagocytosis▪ pHrodo Green E. coli BioParticles
▪ pHrodo Green S. aureus BioParticles
▪ pHrodo Red E. coli BioParticles
▪ pHrodo Red Phagocytosis
   

Green laser (532 nm) reagents

ColorOrangeRedFar RedNear IR
Emission filter575/36 nm620/15 nm695/40 nm780/60 nm
ChannelGL2GL2GL3GL4
Compatible fluorescent proteins▪ tdTomato
▪ YFP
▪ tmCherry  
Antibody labels

▪ Alexa Fluor 532 ▪ Alexa Fluor 555
▪ DsRed
▪ Qdot 565
▪ Qdot 605
▪ R-phycoerythrin (R-PE, PE)

▪ Alexa Fluor 568
▪ Alexa Fluor 594
▪ Qdot 605
▪ PE-Texas Red

▪ Qdot 705
▪ PE-Alexa Fluor 700
▪ TRI-COLOR (PE-Cy5)
▪ PE-Cy5.5

▪ Qdot 800
▪ PE-Cy7

Viability dyes (compatible with fixation) ▪ LIVE/DEAD Fixable Red  
Viability dyes (non-fixed cells)▪ SYTOX Orange▪ SYTOX AADVanced
▪ PI
▪ SYTOX AADVanced 
DNA content/
cell cycle dyes (live cells)
▪ Vybrant DyeCycle Orange▪ Vybrant DyeCycle Orange▪ Vybrant DyeCycle Ruby 
DNA content/
cell cycle dyes (fixed cells)
 ▪ FxCycle PI/Rnase  
Apoptosis dyes▪ Annexin V, R-PE
▪ Annexin V, Alexa Fluor 555
▪ MitoTracker Orange CMTMRos
▪ Annexin V, Alexa Fluor 568
▪ Annexin V, Alexa Fluor 594
▪ MitoTracker Red MNXRos
  
Cell proliferation▪ CellTrace Yellow

▪ Click-iT Plus EDU
▪ Alexa Fluor 594

  
ROS detection▪ CellROX Orange   
Phagocytosis▪ pHrodo Red E. coli BioParticles
   

Yellow laser (561 nm) reagents

ColorOrangeRedFar redNear IR
Emission filter585/16 nm620/15 nm695/40 nm780/60 nm
ChannelYL1YL2YL3YL4
Compatible fluorescent proteins▪ mOrange
▪ RFP
▪ dTomato
▪ mCherry
▪ DsRed
▪ mKate
▪ mStrawberry
  
Antibody labels▪ Alexa Fluor 568
▪ PE
▪ Qdot 565
▪ Qdot 585
▪ Alexa Fluor 594
▪ PE- Alexa Fluor 610
▪ PE-Texas Red
▪ PE-Alexa Fluor 700
▪ PE-Cy5.5
▪ Qdot 705
▪ TRI-COLOR
▪ PE-Alexa Fluor 750
▪ PE-Cy7
▪ Qdot 800
Viability dyes (compatible with fixation)    
Viability dyes (non-fixed cells)▪ PI
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX AADvanced
▪ SYTOX Orange
▪ 7-AAD
▪ PI
▪ SYTOX AADvanced
 
DNA content/cell cycle dyes (live cells)▪ Vybrant DyeCycle Orange  ▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells) ▪ FxCycle PI/Rnase  
Apoptosis dyes▪ Annexin V AF568
▪ Annexin V AF594
▪ MitoTracker Red CMXRos
▪ MitoTracker Orange CMTMRos
▪ TMRM
   
Cell proliferation    
ROS detection▪ CellROX Orange   
Phagocytosis    

Red (637 nm) laser reagents

ColorFar RedFar RedNear IR
Emission filter670/14 nm720/30 nm780/60 nm
ChannelRL1RL2RL3
Compatible fluorescent proteins   
Antibody labels▪ APC
▪ Alexa Fluor 647
▪ Alexa Fluor 660
▪ Qdot 655
▪ Alexa Fluor 680
▪ Alexa Fluor 700
▪ APC-Alexa Fluor 700
▪ Qdot 705
▪ APC-Alexa Fluor 750
▪ APC-Cy7
▪ Qdot 800
Viability dyes (compatible with fixation)▪ LIVE/DEAD Fixable Far Red ▪ LIVE/DEAD Fixable Near-IR
Viability dyes (non-fixed cells)▪ SYTOX Red  
DNA content/cell cycle dyes (live cells)  ▪ Vybrant DyeCycle Ruby
DNA content/cell cycle dyes (fixed cells)▪ FxCycle Far Red  
Apoptosis dyes▪ Annexin V APC
▪ Annexin V AF647
▪ Annexin V AF 680
▪ TO-PRO-3
▪ MitoProbe DilC1(5)
  
Cell proliferation▪ Click-iT Plus Edu Alexa Fluor 647
▪ CellTrace Far Red
  
ROS detection▪ CellROX Deep Red  
Phagocytosis   

Find compatible reagents according to Attune instrument configuration

The antibody labels and fluorescent proteins that are compatible with each Attune Flow Cytometer laser configuration are shown in the expandable sections below.

Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
 
eGFP
Emerald
eYFP
 
574/26BL2
Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
 
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705
TRI-COLOR
 
780/60BL4Pe-Cy7
PE-Alexa Fluor 750
Qdot 800
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Blue–488 nm525/50BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40BL2PE
PE-Alexa Fluor 610
PE-Texas Red
EYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705
TRI-COLOR
 
Green–532 nm575/36GL1Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15GL2Alexa Fluor 568
Alexa Fluor 594
Qdot 605
PE-Texas Red
tmCherry
695/40GL3Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy5)
PE-Cy5.5
 
780/60GL4Qdot 800
PE-Cy7
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40BL2PE
PE-Alexa Fluor 610
PE-Texas Red
Qdot 585
 
695/40BL3PE-Alexa Fluor 700
Tri-Color
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705 
 
Yellow–561 nm585/16YL1PE mOrange
RFP
dTomato
620/15YL2PE- Alexa Fluor 610
PE-Texas Red
mCherry
DsRed
mKate
mStrawberry
695/40YL3PE-Alexa Fluor 700
PE-Cy5.5
Qdot 705
Tri-Color
 
780/60YL4PE-Cy7
Qdot 800
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 436
Super Bright 645
Alexa Fluor 405
Pacific Blue
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25VL2 Pacific GreenvGFP
603/48VL3Pacific Orange
Qdot 605
 
710/50VL4Super Bright 702
Qdot 705
 
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
574/26BL2Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
Tri-Color
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705 
 
780/60BL4Pe-Cy7
PE-Alexa Fluor 750
Qdot 800
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
574/26BL2Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
Tri-Color
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705 
 
780/60BL4Pe-Cy7
PE-Alexa Fluor 750
Qdot 800
 
Red–637 nm670/14RL1APC
Alexa Fluor 647
Qdot 655
 
720/30RL2Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60RL3APC-Alexa Fluor 750
APC-Cy7
Qdot 800
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Blue–488 nm525/50BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40BL2PE
PE-Alexa Fluor 610
PE-Texas Red
eYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705
TRI-COLOR
 
Green—532 nm575/36GL1Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15GL2Alexa Fluor 568
Alexa Fluor 594
Qdot 605
PE-Texas Red
tmCherry
695/40GL3Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy5)
PE-Cy5.5
 
780/60GL4Qdot 800
PE-Cy7
 
Red–637 nm670/14RL1Alexa Fluor 647
APC
Qdot 655
 
720/30RL2Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60RL3APC-Alexa Fluor 750
APC-Cy7
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Blue–488 nm525/50BL1Alexa Fluor 488
FITC
EGFP
Emerald
EYFP
590/40BL2Alexa Fluor 488
FITC
EYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705
TRI-COLOR
 
Green—532 nm575/36GL1Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15GL2Alexa Fluor 568
Alexa Fluor 594
Qdot 605
PE-Texas Red
tmCherry
695/40GL3Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy5)
PE-Cy5.5
 
780/60GL4Qdot 800
PE-Cy7
 
Violet—405 nm440/50VL1 Super Bright 436
Super Bright 645
Alexa Fluor 405
Pacific Blue
Azurite
Cerulean
EBFP
ECFP
mTurquoise
512/25VL2Pacific GreenT-sapphire 
603/48VL3Pacific Orange
Qdot 605
 
710/50VL4Super Bright 702
Qdot 705
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 436
Super Bright 645
Alexa Fluor 405
Pacific Blue
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25VL2 Pacific GreenvGFP
603/48VL3Pacific Orange
Qdot 605
 
710/50VL4Super Bright 702
Qdot 705
 
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40BL2PE
PE-Alexa Fluor 610
PE-Texas Red 
Qdot 585
 
695/40BL3PE-Alexa Fluor 700
Tri-Color
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705 
 
Yellow–561 nm585/16YL1PE mOrange
RFP
dTomato
620/15YL2PE- Alexa Fluor 610
PE-Texas Red
mCherry
DsRed
mKate
mStrawberry
695/40YL3PE-Alexa Fluor 700
PE-Cy5.5
Qdot 705
Tri-Color
 
780/60YL4PE-Cy7
Qdot 800
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 436
Super Bright 645
Alexa Fluor 405
Pacific Blue
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25VL2 Pacific GreenvGFP
603/48VL3Pacific Orange
Qdot 605
 
710/50VL4Super Bright 702
Qdot 705
 
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
574/26BL2Alexa Fluor 546
PE
Qdot 565
Qdot 585
eYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
Tri-Color
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705 
 
780/60BL4Pe-Cy7
PE-Alexa Fluor 750
Qdot 800
 
Red–637 nm670/14RL1APC
Alexa Fluor 647
Qdot 655
 
720/30RL2Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60RL3APC-Alexa Fluor 750
APC-Cy7
Qdot 800
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 436
Super Bright 645
Alexa Fluor 405
Pacific Blue
Azurite
Cerulean
EBFP
ECFP
mTurquoise
512/25VL2Pacific Green



T-Sapphire
603/48VL3Pacific Orange
Qdot 605
 
710/50VL4Super Bright 702
Qdot 705
 
Blue–488 nm525/50BL1Alexa Fluor 488
FITC
EGFP
Emerald
EYFP
590/40BL2PE
PE-Alexa Fluor 610
PE-Texas Red
EYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705
 
Green—532 nm575/36GL1Alexa Fluor 532
Alexa Fluor 555
DsRed
Qdot 565
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
YFP
620/15GL2Alexa Fluor 568
Alexa Fluor 594
Qdot 605 PE-Texas Red
tmCherry
695/40GL3Qdot 705
PE-Alexa Fluor 700
TRI-COLOR (PE-Cy5)
PE-Cy5.5
 
780/60GL4Qdot 800
PE-Cy7
 
Red–637 nm670/14RL1Alexa Fluor 647
APC
Qdot 655
 
720/30RL2Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60RL3APC-Alexa Fluor 750
APC-Cy7
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 436
Super Bright 645
Alexa Fluor 405
Pacific Blue
Sirius
Azurite
eCFP
eBFP
mTarquoise
Cerulean
512/25VL2 Pacific GreenvGFP
603/48VL3Pacific Orange
Qdot 605
 
710/50VL4Super Bright 702
Qdot 705
 
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
590/40BL2PE
PE-Alexa Fluor 610
PE-Texas Red 
Qdot 585
 
695/40BL3PE-Alexa Fluor 700
Tri-Color
PE-Cy5.5
PerCP
PerCP-Cy5.5
Qdot 705 
 
Yellow–561 nm585/16YL1PE mOrange
RFP
dTomato
620/15YL2PE- Alexa Fluor 610
PE-Texas Red
mCherry
DsRed
mKate
mStrawberry
695/40YL3PE-Alexa Fluor 700
PE-Cy5.5
Qdot 705
Tri-Color
 
780/60YL4PE-Cy7
Qdot 800
 
Red–637 nm670/14RL1APC
Alexa Fluor 647
Qdot 655
 
720/30RL2Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60RL3APC-Alexa Fluor 750
APC-Cy7
Qdot 800
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 645
Super Bright 436
eFluor 450
VioBlue
Brilliant Violet 421
Pacific Blue
BD Horizon V450
Azurite
Cerulean
eBFP
eCFP
mTurquoise
Sirius
512/25VL2eFluor 506
VioGreen
Brilliant Violet 510
Pacific Green
BD Horizon V500
T-Sapphire
CFP
vGFP
603/48VL3Super Bright 600
Qdot 605
Pacific Orange
Brilliant Violet 605
 
660/20VL4Super Bright 645
Brilliant Violet 650
 
710/50VL5 Super Bright 702
Brilliant Violet 711
Qdot 705
 
780/60VL6Super Bright 786
Brilliant Violet 786
 
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
574/26BL2PE
Alexa Fluor 546
PE-Alexa Fluor 610
PE-Texas Red
PE-eFluor 610
eYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
Qdot 705
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 645
Super Bright 436
eFluor 450
VioBlue
Brilliant Violet 421
Pacific Blue
BD Horizon V450
Azurite
Cerulean
eBFP
eCFP
mTurquoise
Sirius
512/25VL2eFluor 506
VioGreen
Brilliant Violet 510
Pacific Green
BD Horizon V500
T-Sapphire
CFP
vGFP
603/48VL3Super Bright 600
Qdot 605
Pacific Orange
Brilliant Violet 605
 
660/20VL4Super Bright 645
Brilliant Violet 650
 
710/50VL5Super Bright 702
Brilliant Violet 711
Qdot 705
 
780/60VL6Super Bright 786
Brilliant Violet 786
 
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
574/26BL2PE
Alexa Fluor 546
PE-Alexa Fluor 610
PE-Texas Red
PE-eFluor 610
eYFP
mCitrine
Venus
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
Qdot 705
 
Red–637 nm670/14RL1APC
Alexa Fluor 647
Qdot 655
eFluor 660
 
720/30RL2Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60RL3APC-Alexa Fluor 750
APC-Cy7
Qdot 800
APC-eFluor 780
 
Excitation laserEmission filter (nm)ChannelAntibody labelsCompatible fluorescent proteins
Violet–405 nm440/50VL1 Super Bright 645
Super Bright 436
eFluor 450
VioBlue
Brilliant Violet 421
Pacific Blue
BD Horizon V450
Azurite
Cerulean
eBFP
eCFP
mTurquoise
Sirius
512/25VL2eFluor 506
VioGreen
Brilliant Violet 510
Pacific Green
BD Horizon V500
T-Sapphire
CFP
vGFP
603/48VL3Super Bright 600
Qdot 605
Pacific Orange
Brilliant Violet 605
 
660/20VL4Super Bright 645
Brilliant Violet 650
 
710/50VL5Super Bright 702
Brilliant Violet 711
Qdot 705
 
780/60VL6Super Bright 786
Brilliant Violet 786
 
Blue–488 nm530/30BL1Alexa Fluor 488
FITC
eGFP
Emerald
eYFP
695/40BL3PE-Alexa Fluor 700
PE-Cy5.5
PerCP-Cy5.5
PerCP-eFluor 710
Qdot 705
 
Yellow–561 nm585/16YL1Alexa Fluor 555
Qdot 605
R-phycoerythrin (R-PE, PE)
tdTomato
mOrange
YFP
RFP
620/15YL2Alexa Fluor 568
Alexa Fluor 594
Qdot 605
PE-Texas Red
PE-Alexa Fluor 610
PE-eFluor 610
mCherry
DsRed
mKate
mStrawberry
780/60YL4Qdot 800
PE-Cy7
PE-Alexa Fluor 750
 
Red–637 nm670/14RL1APC
Alexa Fluor 647
Qdot 655
eFluor 660
 
720/30RL2Alexa Fluor 680
Alexa Fluor 700
APC-Alexa Fluor 700
Qdot 705
 
780/60RL3APC-Alexa Fluor 750
APC-Cy7
Qdot 800
APC-eFluor 780
 

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.