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Rat pituitary intermediate lobe melanotropes labeled with the indo-1 AM (Cat. no. I1203, I1223, I1226) and carboxy SNARF®-1, AM, acetate (Cat. no. C1271, C1272) indicators. Pseudocolored fluorescence from the dual-emission Ca2+ indicator indo-1 is shown at 405 and 475 nm (left panels). Pseudocolored fluorescence from the dual-emission pH indicator carboxy SNARF®-1 is shown at 575 and 640 nm (right panels). Image contributed by Stephen J. Morris, University of Missouri-Kansas City, and Diane M. Beatty, Molecular Probes, Inc.