Photobleaching of fluorescein phalloidin in bovine pulmonary artery endothelial cells with and without the ProLong® Antifade mounting medium.

Bovine pulmonary artery endothelial cells were labeled with fluorescein phalloidin (Cat. no. F432), which labels filamentous actin, and placed under constant illumination on the microscope with a FITC filter set using a 60× objective. Images were acquired at one-second intervals for 30 seconds. Under these illumination conditions, fluorescein photobleached to about 12% of its initial value in 30 seconds in PBS (left), but stayed at the initial value under the same illumination conditions when mounted using the reagents in the ProLong® Antifade Kit (right, Cat. no. P7481).

Bovine pulmonary artery endothelial cells were labeled with fluorescein phalloidin (Cat. no. F432), which labels filamentous actin, and placed under constant illumination on the microscope with a FITC filter set using a 60× objective. Images were acquired at one-second intervals for 30 seconds. Under these illumination conditions, fluorescein photobleached to about 12% of its initial value in 30 seconds in PBS (left), but stayed at the initial value under the same illumination conditions when mounted using the reagents in the ProLong® Antifade Kit (right, Cat. no. P7481).

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