Bovine pulmonary artery endothelial cells labeled with probes for actin, mitochondria and the phosphorylated form of histone H3.

Bovine pulmonary artery endothelial cells labeled with probes for actin, mitochondria and the phosphorylated form of histone H3. Actin was labeled with blue-fluorescent Alexa Fluor® 350 phalloidin (Cat. no. A22281), and phosphorylated histone H3 was detected using a rabbit anti–phosphohistone H3 antibody prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (red fluorescence, Cat. no. Z25305). Endogenous biotin associated with the mitochondria was labeled using streptavidin (Cat. no. S888) followed by a rabbit anti-streptavidin antibody prelabeled with the Zenon® Alexa Fluor® 488 Rabbit IgG Labeling Kit (green fluorescence, Cat. no. Z25302).

Bovine pulmonary artery endothelial cells labeled with probes for actin, mitochondria and the phosphorylated form of histone H3. Actin was labeled with blue-fluorescent Alexa Fluor® 350 phalloidin (Cat. no. A22281), and phosphorylated histone H3 was detected using a rabbit anti–phosphohistone H3 antibody prelabeled with the Zenon® Alexa Fluor® 555 Rabbit IgG Labeling Kit (red fluorescence, Cat. no. Z25305). Endogenous biotin associated with the mitochondria was labeled using streptavidin (Cat. no. S888) followed by a rabbit anti-streptavidin antibody prelabeled with the Zenon® Alexa Fluor® 488 Rabbit IgG Labeling Kit (green fluorescence, Cat. no. Z25302).

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