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Peroxisomes were labeled using an antibody directed at peroxisomal membrane protein 70 (PMP-70) and detected with Alexa Fluor® 488 dye–labeled goat anti–mouse IgG secondary antibody. Mitochondria were stained with MitoTracker® Red CMXRos (Cat. no. M7512) prior to fixation; nuclei were stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490).