Detection of oxidative stress in live cells using the Image-iT® LIVE Reactive Oxygen Species (ROS) Kit.

Detection of oxidative stress in live cells using the Image-iT® LIVE Reactive Oxygen Species (ROS) Kit (I36007). Live bovine pulmonary artery endothelial cells were treated with tert-butyl hydroperoxide to induce oxidative stress (bottom) or were left untreated (top). Cells were then labeled with carboxy-H2DCFDA, which fluoresces when oxidized by ROS, and nuclei were stained with blue-fluorescent Hoechst 33342. The stressed cells exhibited green fluorescence, signaling an increase in ROS, whereas the untreated cells showed minimal fluorescence.

Detection of oxidative stress in live cells using the Image-iT® LIVE Reactive Oxygen Species (ROS) Kit (I36007). Live bovine pulmonary artery endothelial cells were treated with <i>tert</i>-butyl hydroperoxide to induce oxidative stress (bottom) or were left untreated (top). Cells were then labeled with carboxy-H<sub>2</sub>DCFDA, which fluoresces when oxidized by ROS, and nuclei were stained with blue-fluorescent Hoechst 33342. The stressed cells exhibited green fluorescence, signaling an increase in ROS, whereas the untreated cells showed minimal fluorescence.

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