Resources for Previous Models of Qubit Fluorometers

Parent Name

User defined, max 10 characters

Indicates the name visible on the initial home screen, i.e., the “family” of assays.  For example, “DNA” is a Parent Name on the factory-configured instrument.

Name1 (and Name2, 3, 4)

User defined, max 10 characters

Indicates the individual assay name. For example, “dsDNA” is a Name on the factory-configured instrument.

Name Sub-text

User defined, max 20 characters

Indicates any smaller sub-text under the assay name (or to the right of the assay name from the graphical display). For example, “High Sensitivity” is a Name Sub-text on the factory-configured instrument.

Calibration

YES or NO

Indicates whether you are creating an assay that requires calibration or not. “YES” indicates that the assay will require calibration. All assays created using MyQubit that require calibration will utilize two standards, the first of which is always a sample blank (analyte concentration of “0”) and the second of which is a sample of a concentration to be determined by the user. “NO” indicates that the assay will not require calibration. In this case, the output will be a raw fluorescence value or values.

Units

User defined, text only, max 10 characters

Required only when Calibration is “YES.”  Maximum length 10 characters.

Excitation

BLUE, RED, BOTH, or NONE

BOTH and NONE are acceptable only if Calibration is “NO.”  Otherwise, this constitutes an error, and the .qbt file will not upload. BLUE = 470 nm, filter 430–495 nm. RED = 635 nm, filter 600–645 nm.

Emission

GREEN, FAR RED, or BOTH

BOTH is acceptable only if Calibration is “NO.”  Otherwise, this constitutes an error and the .qbt file will not upload. GREEN = 510–580 nm. FAR RED = 665–725 nm.

Std Signal Ratio Limit

User defined, numeric only

Needed only when Calibration is “YES.” This number specifies the amount, in raw fluorescence, that the signal for Standard #2 must exceed the signal for Standard #1 for your MyQubit assay. This is meant to help troubleshoot large oversights in calibration.  If you are unsure of a reasonable threshold, or if this is not of concern to you, then enter a small value that you will be assured of attaining in most calibrations.

Low

User defined, numeric only

Needed only when Calibration is “YES.” Indicates the lowest sample concentration that the instrument will display a result for. Samples with concentrations below this value will be reported as “< Low” and will display the message “*Sample TOO LOW*”

High

User defined, numeric only

Needed only when Calibration is “YES.” Indicates the highest sample concentration that the instrument will display a result for. Samples with concentrations above this value will be reported as “> High” and will display the message “*Sample TOO HIGH*” 

N

User defined, numeric only

Needed only when Calibration is “YES.” One of three algorithm constants that must be determined by the user. Indicates the linearity of the curve, with a value of 1.00 corresponding to completely linear.

K

User defined, a scaling factor, numeric only

Needed only when Calibration is “YES.” One of three algorithm constants that must be determined by the user. Indicates the portion of the curve to focus on. 

S

User defined, numeric only

Needed only when Calibration is “YES.” One of three algorithm constants that must be determined by the user. Indicates the concentration value of Standard #2 used in assay calibration.

Green RFU Name

User defined, max 20 characters

Indicates the name given to the displayed RFU output for Green emission when Calibration is “NO.” Needed only when Calibration is “NO” and Emission is “GREEN” or “BOTH.”

Far Red RFU Name

User defined, max 20 characters

Indicates the name given to the displayed RFU output for Far Red emission when Calibration is “NO.” Needed only when Calibration is “NO” and Emission is “FAR RED” or “BOTH.”