Bovine pulmonary artery endothelial cells (BPAEC).

Bovine pulmonary artery endothelial cells simultaneously stained with LysoTracker® Red DND-99 (Cat. No. L7528), a cell-permeant, fixable lysosomal stain, and with 5-(pentafluorobenzoylamino)fluorescein di-β-D-galactopyranoside (PFB-FDG, Cat. No. P11948), a fluorogenic substrate for beta-galactosidase. PFB-FDG is nonfluorescent until enzymatically hydrolyzed to green-fluorescent PFB-F. The center image demonstrates colocalization of the LysoTracker® Red DND-99 dye and PFB-F to the lysosomes. The left image was acquired with a bandpass filter set appropriate for fluorescein, the right image was acquired with a bandpass filter set appropriate for Texas Red® dye, and the center image was acquired with a double bandpass optical filter set appropriate for fluorescein and the Texas Red® dye.