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2020 Attune NxT Flow Cytometer Violet 6-Channel Option
Expand your violet laser capabilities
The 2020 Invitrogen Attune NxT Flow Cytometer with acoustic focusing technology is now available with 6 fluorescence detectors off the violet laser, allowing measurement of up to 11 fluorescence parameters with a 2-laser instrument, 12 fluorescence parameters with a 3-laser instrument, or 14 fluorescence parameters with a 4-laser instrument (Table 1).
- 6 fluorescence channels, can accommodate the Super Bright antibody conjugates
- Detect more data from a single sample and streamline panel design
- Help save time and sample with less clogging
Table 1. Attune NxT Flow Cytometer configuration using six fluorescence detectors for the violet laser.
| Laser | Fluorescence detectors | ||
|---|---|---|---|
| 2-laser | 3-laser | 4-laser | |
| Violet, 405 nm | 6 | 6 | 6 |
| Blue, 488 nm | 3 | 3 | 2 |
| Yellow, 561 nm | NA | NA | 3 |
| Red, 637 nm | NA | 3 | 3 |
| Total fluorescence detectors available | 9 | 12 | 14 |
| Total parameters per configuration* | 11 | 14 | 16 |
*Includes forward scatter (FSC) and side scatter (SSC). NA = not applicable.
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Table 2. Attune NxT Flow Cytometer configuration options.
| Lasers | Laser configuration (Cat. No.) | Lasers included, No. of detection channels | Total detection channels* | ||||
|---|---|---|---|---|---|---|---|
| Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm | Red, 637 nm | |||
| 1 | Blue (A24864) | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
| 2 | Blue/green (A28995) | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | |
| Blue/yellow (A24861) | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | ||
| Blue/red (A24863) | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† |
3
| 9 | |
| Blue/violet (A24862) |
4
|
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
| Blue/violet 6 (A29002) |
6
|
3
| Available as upgrade† | Available as upgrade† | 11 | ||
| 3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
| 12 | |
| Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
| Blue/green/violet (A28999) |
4
|
3
|
4
| Available as upgrade† | 13 | ||
| Blue/violet/yellow (A24859) |
4
|
3
|
4
| Available as upgrade† | 13 | ||
| Blue/red/violet (A24860) |
4
|
4
| Available as upgrade† | Available as upgrade† |
3
| 13 | |
| Blue/red/violet 6 (A29003) |
6
|
3
| Available as upgrade† |
3
| 14 | ||
| 4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
| 16 | |
| Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
| 16 | ||
| Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
| 16 | ||
| * Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. | |||||||
The 2018 Attune NxT Flow Cytometer 6-channel violet laser configuration accommodates the Invitrogen eBioscience Super Bright antibody conjugates along with other commercially available violet-excitable fluorophores.
| Detector | Bandpass (nm) | Fluorophores* |
|---|---|---|
| VL1 | 450/40 | Super Bright 436, Brilliant Violet 421, eFluor 450, Pacific Blue, BD Horizon V450, VioBlue |
| VL2 | 525/50 | eFluor 506, Brilliant Violet 510, Pacific Green, BD Horizon V500, VioGreen |
| VL3 | 610/20 | Super Bright 600, Brilliant Violet 605, Pacific Orange |
| VL4 | 660/20 | Super Bright 645, Brilliant Violet 650 |
| VL5 | 710/50 | Super Bright 702, Brilliant Violet 711 |
| VL6 | 780/60 | Brilliant Violet 786 |
* List is not inclusive of all available fluorophores.
Brochure
Poster
Specification sheet
Table 2. Attune NxT Flow Cytometer configuration options.
| Lasers | Laser configuration (Cat. No.) | Lasers included, No. of detection channels | Total detection channels* | ||||
|---|---|---|---|---|---|---|---|
| Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm | Red, 637 nm | |||
| 1 | Blue (A24864) | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
| 2 | Blue/green (A28995) | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | |
| Blue/yellow (A24861) | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | ||
| Blue/red (A24863) | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† |
3
| 9 | |
| Blue/violet (A24862) |
4
|
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
| Blue/violet 6 (A29002) |
6
|
3
| Available as upgrade† | Available as upgrade† | 11 | ||
| 3 | Blue/green/red (A28997) |
Available as upgrade†
|
3
|
4
|
3
| 12 | |
| Blue/red/yellow (A28993) | Available as upgrade† | 3 | 4 | 3 | 12 | ||
| Blue/green/violet (A28999) |
4
|
3
|
4
| Available as upgrade† | 13 | ||
| Blue/violet/yellow (A24859) |
4
|
3
|
4
| Available as upgrade† | 13 | ||
| Blue/red/violet (A24860) |
4
|
4
| Available as upgrade† | Available as upgrade† |
3
| 13 | |
| Blue/red/violet 6 (A29003) |
6
|
3
| Available as upgrade† |
3
| 14 | ||
| 4 | Blue/red/violet/green (A29001) |
4
|
3
|
4
|
3
| 16 | |
| Blue/red/yellow/violet (A24858) |
4
|
3
|
4
|
3
| 16 | ||
| Blue/red/yellow/violet 6 (A29004) |
6
|
2
|
3
|
3
| 16 | ||
| * Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. | |||||||
The 2018 Attune NxT Flow Cytometer 6-channel violet laser configuration accommodates the Invitrogen eBioscience Super Bright antibody conjugates along with other commercially available violet-excitable fluorophores.
| Detector | Bandpass (nm) | Fluorophores* |
|---|---|---|
| VL1 | 450/40 | Super Bright 436, Brilliant Violet 421, eFluor 450, Pacific Blue, BD Horizon V450, VioBlue |
| VL2 | 525/50 | eFluor 506, Brilliant Violet 510, Pacific Green, BD Horizon V500, VioGreen |
| VL3 | 610/20 | Super Bright 600, Brilliant Violet 605, Pacific Orange |
| VL4 | 660/20 | Super Bright 645, Brilliant Violet 650 |
| VL5 | 710/50 | Super Bright 702, Brilliant Violet 711 |
| VL6 | 780/60 | Brilliant Violet 786 |
* List is not inclusive of all available fluorophores.
Brochure
Poster
Specification sheet
Resources
Meet the Inventor video series
Support
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Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.