Innovative Cell Biology Solutions

Upgrade your cell biology with optimized solutions


We go beyond basic formulations to provide optimized and innovative cell biology solutions in support of your efforts to achieve consistently reproducible results.

 

Gibco, Invitrogen and Nunc cell culture products are designed to deliver reproducibility and performance for results you can count on. Regardless of whether you are performing cell culture in a research lab or production facility, need a special or defined formulation, or are growing cell lines, primary cells, or stem cells, Gibco, Invitrogen and Nunc products offer a reliable solution.
 

Explore all cell biology innovations

Select the product type below to view relevant innovations to support your cutting-edge work and help you push boundaries confidently.

Cell culture media & FBS innovations

Time-tested and trusted, Gibco cell culture media, FBS and reagents are designed to support the growth and maintenance of a variety of mammalian, insect and microbial cells and cell lines.

 

Explore Gibco media for research use at thermofisher.com/gibcomedia and for production use at thermofisher.com/gibcobioprocessing

 

Discover the performance differences that Gibco FBS can deliver for your research and production at thermofisher.com/fbs

The Gibco media bottle

Designed to provide better ergonomic features, ease of use, and reduced chance of contamination. The compact design of the Gibco media bottle allows for easier handling and storage, and is fully recyclable with PET bottle and HDPE cap.

Gibco BenchStable Media

Engineered for flexibility and convenience to enable storage at room temperature. Store it anywhere, on your lab bench or in the refrigerator. Available in the most used basal media formulations: DMEM, DMEM/F-12, MEM, and RPMI 1640. BenchStable media have been optimized for routine cell culture, maintaining expected morphology and function of many common cell lines.

Gibco GlutaMAX Supplement

More stable than L-glutamine for keeping your cells healthier longer. Gibco GlutaMAX Supplement is a stabilized form of L-glutamine and features exceptional stability to help prevent degradation during storage or in the presence of cells. Available as a stand-alone supplement, as a component in Gibco media products, and within antibiotic Pen-Strep-GlutaMAX supplement.

Gibco TrypLE Cell Dissociation Reagents

Cell-dissociation enzymes that replace porcine trypsin. TrypLE reagents are gentle on cells, protecting your cell’s surface proteins. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

Gibco Human Plasma-like Medium

A formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. Gibco HPLM provides researchers the ability to study the impact of physiologically relevant cell media on their specific applications.

Gibco OncoPro Tumoroid Culture Medium Kit

Streamlines the culture of patient-derived cancer organoids (tumoroids) to help make physiologically relevant cancer models more accessible. This adaptable culture system is readily integrated into your workflow so you can upgrade your media and maintain your results.

Gibco One Shot FBS 50 mL Bottle

One Shot FBS 50 mL bottle was designed specifically for FBS use to minimize contamination and save time. This unique little bottle makes it possible to eliminate the need to aliquot and reduce plastic waste by 33%. Discover why so many researchers love the benefits that come with this One Shot bottle.

Origin and traceability reassurance

We’ve been ISIA Traceability Certified since 2014, and we were the first serum supplier to provide fetal bovine serum (FBS) fingerprinting technology powered by Oritain to provide empirical and statistical certainty over the origin and authenticity. We consider it imperative that our customers know exactly what they‘re getting and have confidence that our products will enable the results they demand.

Gibco Advanced Granulation Technology format (AGT)

The AGT medium format was developed to help overcome multi-step dry powder media preparation and filtration challenges and to decrease handling safety risks. AGT granules dissolve rapidly to decrease media preparation time. The AGT dry media format is designed to provide increased consistency and productivity across all stages of production.

Gibco Freedom kits for cloning and expression

Gibco Freedom kits contain all the components for beginning-to-end cloning and expression, within a short development time. Each kit includes cGMP compliant products, with no need to source components or develop vectors, allowing you to focus on quickly moving your workflow from cell line to process development. Our Freedom kits also offer commercial licensing that’s simple, flexible, and without royalties.

Cell culture plastics innovations

To optimize results for different cell culture types, a range of cell culture surfaces and a variety of formats are available for every step of your workflow. Explore all cell culture plastics and find cultureware by surface, format, or application need at thermofisher.com/cellcultureplastics

Nunclon Supra surfaces for advanced cell culturing

The Nunclon Supra product line is an innovative surface treatment that will support improved cell yield and morphology with reduced adherence time as well enabling you to do xeno-free or low-serum applications for MSCs and difficult-to-culture cells.

 

See how our surfaces perform with 39 cell types and cell lines at thermofisher.com/supradata

 

Learn about Supra surfaces:  Dishes   |  Flasks  |  Plates  |  96-well plates

Nunc UpCell surfaces for adherent cell culture

The Nunc UpCell surface is designed to be temperature responsive, enabling non-enzymatic cell dissociation. There’s no need for trypsin, nor scraping—UpCell non-enzymatic surfaces mean better preservation of cell surface proteins and enable improved cell viability. With supportive membrane options, UpCell surfaces enable harvesting of cell sheets and the creation of 3D tissue models.

Nunclon Sphera surface for low-attachment 3D cell culture

Nunclon Sphera 3D culture system is designed for spheroid culture (e.g., 3D tumor spheroids) and organoid culture (e.g., brain organoids) with a wide selection of formats, from plates to dishes and flasks. Low attachment cell culture plates allow virtually no cell attachment to the cultureware, supporting the establishment of 3D spheroids and organoids via cell-to-cell aggregation through naturally secreted extracellular matrices (ECM).

Pluripotent stem cell culture innovations

For decades, Thermo Fisher Scientific has been developing innovative Gibco stem cell culture media and reagents to support diverse workflows—enabling scientists to push the boundaries of their stem cell research.

 

Discover all stem cell culture solutions at thermofisher.com/stemcellculture

Gibco CTS StemScale PSC Suspension Medium

A xeno-free medium designed to support the scalable expansion of PSCs in suspension culture for translational and clinical applications. Achieve 30% higher expansion vs other PSC suspension media across multiple culture vessel formats and sizes, from small to large scale.

Gibco CultureCEPT Supplement

Gibco CultureCEPT Supplement reduces cellular stress and improves viability of stem cells, differentiated cells, and primary cells during stressful cell culture processing. It is a unique formulation that provides exceptional cell protection compared to traditional ROCK inhibitors.

Gibco StemFlex Medium

Designed for today's most challenging applications; 2-fold faster recovery following gene editing, 5-fold improvement in clonal expansion following single-cell passaging, and maximal workflow flexibility.

Gibco Essential 8 media

Fully defined, xeno-free formulations containing only the 8 essential components needed to grow feeder-free. Versions available to enable weekend-free feeding and cell therapy research. For cell therapy development, try CTS Essential 8 Medium.

Gibco KnockOut Serum Replacement

A defined medium designed to replace FBS in feeder-dependent ESC and iPSC cultures. For cell therapy development, try CTS KnockOut SR XenoFree Medium.

Neuronal cell culture innovations

Our Gibco neuronal and neural stem cell culture media and supplements are the most widely used for neural cell culture. All of our products have been developed to work together for optimal performance and to create complete neuronal, glial, and neural stem cell culture systems.

 

Discover all neurobiology research solutions at thermofisher.com/neuro

Gibco B-27 Plus Supplement

The most-referenced neuronal cell culture supplement just got better. To further meet the growing needs of researchers, an optimized formulation of the classic B-27 Supplement was developed in 2018 for improved long-term neuronal survival, functionality and maturation of primary rodent neurons and stem cell-derived neurons.

Gibco B-27 Supplement

The first commercially available, serum-free supplement for neural cell culture, Gibco B-27 Supplement (50X) has set the standard in neuronal cell culture reagents. Originally designed for culturing primary neurons, the classic B-27 Supplement and nutritive variants have been widely adapted and successfully used in many other applications and cell types, including neural stem cells and PSC-derived neurons, and disease modeling. For cell and gene therapy development, try Gibco CTS B-27 Supplement

Gibco Neurobasal Media

Provides long-term maintenance of the normal phenotype and growth of neuronal cells, maintaining pure populations of neuronal cells without the need for an astrocyte feeder layer. Gibco Neurobasal Plus Medium is the next-generation version of Neurobasal, designed to enable the highest in vitro survival of primary rodent and human stem cell–derived neurons when used with B-27 Plus Supplement. For cell and gene therapy development, try Gibco CTS Neurobasal Media

Transfection innovations

Thermo Fisher Scientific is a trusted supplier of products for highly effective cell transfection. Select from this range of products built to support in vitro transfection of cells in your lab. If you are seeking solutions for in vivo delivery, discover Invitrogen reagents optimized for use in live organisms.

 

Discover transfection solutions at thermofisher.com/transfection

Invitrogen Neon NxT Electroporation System

Protect your valuable research time and samples. The combination of the Invitrogen Neon NxT Electroporation System’s proprietary pipette tip technology, simplified workflow, and flexibility helps reduce sample loss, lowers contamination risk, saves you steps, and can put you in control of your experiments.

Invitrogen Lipofectamine 3000, MessengerMAX, and RNAiMAX reagents

Since launching in 1993, Invitrogen Lipofectamine reagents have become the most referenced transfection reagents with over 50,000 citations. Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use.

Protein expression system innovations

Using the right protein expression system for your specific application is critical to success. We offer a wide selection of mammalian, insect, and other host systems to suit your research needs.

 

Find your system at thermofisher.com/proteinexpression

Gibco ExpiSf Expression System

The first-ever chemically defined baculovirus expression system that can generate three times more protein compared to existing insect expression platforms. Get greater consistency across multiple expression runs, all in less time.

Gibco ExpiCHO Expression System

Maintain protein quality and function from research to bioproduction. This completely optimized system provides the highest yields possible in a transient system (up to 3 g/L). That means you can start your research work in CHO cells and stay in CHO cells through discovery.

Gibco Expi293 Expression System

Trust the industry standard for rapid, high-yield transient HEK 293 expression. The components of this rapid, high-yield protein production system work together to allow access to recombinant 293-derived proteins in just 5-7 days, with a 2–10-fold increase in protein yields.

Cell Therapy Systems (CTS) innovations

Supporting your move from basic cell therapy research to the clinic, Cell Therapy Systems (CTS) products provide a proven choice for cell-based immunotherapy research, clinical stem cell therapy and manufacturing. In addition to providing serum-free, xeno-free and animal origin-free reagent formulations and innovative cell processing instruments our GMP-grade CTS products have cell and gene therapy specific intended use statements, extensive safety testing and proactive regulatory documentation.

 

Discover all clinical cell therapy manufacturing solutions at thermofisher.com/CTS

Gibco CTS AIM V SFM

Gibco CTS AIM V SFM is the first commercially available, defined, serum-free medium (SFM) for the proliferation and/or manipulation of T cells and dendritic cells manufactured in compliance with cGMP.

Gibco CTS AAV-MAX Helper Free AAV Production System

One complete AAV production system for facilitating a seamless translation from research to clinical and commercial production. Increases productivity and cost efficiency by delivering high viral titers using Gibco CTS Viral Production Cells 2.0, a clonally documented, 293F-derived mammalian cell line.

Gibco CTS NK-Xpander Medium

Designed to proliferate a high yield of functional human natural killer (NK) cells with or without the use of feeder cells, CTS NK-Xpander Medium was developed to address the needs of translational and clinical allogeneic NK cell therapy researchers.

Gibco CTS Rotea Counterflow Centrifugation System

The multipurpose Gibco CTS Rotea Counterflow Centrifugation System offers exceptional flexibility in a cell therapy development and manufacturing system. This versatile system applies the proven counterflow centrifugation method to a broad range of cell processing applications, such as CAR-T therapy, stem cell therapy, and PBMC isolation.

Gibco CTS bagged media

Many of our Gibco CTS reagents are now offered in a BioProcess Container format suitable for a closed system workflow to help you close and scale your process, reducing the risk of contamination or human error and increasing your manufacturing efficiency. This can help boost the likelihood of clinical and commercial manufacturing success while also reducing the need for costly Class A clean room use.

Gibco CTS LV-MAX Lentiviral Production System

A complete, optimized serum-free system designed to support high-titer lentiviral production in a variety of suspension culture vessels, enabling you to scale up or down based on your needs. This cost-effective and scalable lentiviral manufacturing platform can support high throughput early in discovery or seamless clinical scale-up, all while maintaining high yield.

Gibco CTS OpTmizer Pro SFM

The first medium developed for the growth and expansion of human T lymphocytes (e.g., CD4+, CD8+, polyclonal, or antigen-specific T cells) used in allogeneic cell therapies. This serum-free and xeno-free medium improves central memory phenotype and cell growth, resulting a larger population of central memory cells in a shorter period.

Gibco CTS Xenon system

This large-volume electroporation system designed for cell therapy development and manufacturing, offers reliably high transfection performance in 1 or 5-25 mL with exceptional cell viability and recovery. The intuitive, programmable interface, process flexibility, sterile single-use consumables, and available software upgrade that helps enable 21 CFR Part 11 compliance allow the system to seamlessly scale with your cell therapy workflow.

Gibco CTS Dynabeads CD3/CD28

Magnetic beads are a trusted technology for the isolation, activation, and expansion of T cells ex vivo. Dynabeads CD3/CD28 can be used for the isolation, activation, and/or expansion of polyclonal T cells, antigen-specific T cells, gene-modified T cells, and other T cell subtypes and will deliver 100- to 1,000-fold expansion in 9–14 days.

Gene editing innovations

To help researchers in their quest to understand how the genome influences phenotype, we’ve developed a complete set of trusted solutions for every step in the gene editing workflow. Our optimized, validated products, protocols, and tools work together to eliminate the trial-and-error phase and help you get answers faster and with less effort.

 

Explore all gene editing solutions at thermofisher.com/geneediting

Invitrogen TrueDesign Genome Editor

This free online tool enables scientists of all experience levels to easily design, select, and order reagents for accurate and successful gene editing experiments.

Invitrogen TrueGuide Synthetic gRNA

Ready-to-transfect guide RNAs (gRNAs) designed and validated to provide specific and high-efficiency knockout of your target gene. Our free software offers easy ordering of custom and pre-designed gRNAs for precise genome editing.

Invitrogen TrueTag Donor DNA Kits

The fast and easy way to generate transfection-ready donor DNA for fluorescent tags, epitope tags, or precise gene editing experiments. Our TrueTag kits maximize editing efficiency and significantly reduce protocol time with no cloning step required.

Invitrogen TrueCut Cas9 proteins

Choose from our next-generation of wild-type and high-fidelity Cas9 proteins, including TrueCut Cas9 Protein v2, engineered to consistently deliver maximum editing efficiency, and TrueCut HiFi Cas9 Protein, designed to significantly reduce off-target edits. We also have Cas9 proteins available to enable your cell therapy development. For cell and gene therapy development, try Gibco CTS Cas9 proteins

Recombinant protein innovations

Whether conducting basic research or developing next generation engineered cell therapies, you need to be able to rely on the quality and integrity of the recombinant proteins used in your experiments. Thermo Fisher Scientific offers a large section of high-quality Gibco PeproTech proteins to meet these needs.

 

Discover recombinant proteins for your research at thermofisher.com/recombinantproteins

Gibco heat stable recombinant proteins

Heat-stable recombinant proteins have been engineered for greater stability in cell culture, exhibiting enhanced bioactivity at 37°C. Because Heat Stable proteins are bioactive longer than native proteins in standard cell culture conditions, researchers may be able to use less to maintain the same growth rates, therefore improving the efficiency of their cell culture applications.

Gibco Peprotech GMP Proteins

Gibco PeproGMP recombinant proteins are manufactured for use as ancillary materials in clinical manufacturing. Considerations such as quality control, documentation, and technical support are center to your bench-to-clinic innovations. PeproGMP recombinant proteins are designed to help you deliver results quickly.

Resources & support

Resources

Cell Culture & Transfection Learning Center

Access cell culture and transfection educational resources for better experiment planning and execution.

 

Gibco Cell Culture Basics
Learn the fundamentals of cell culture for achieving consistent results, including laboratory setup, safety, and aseptic techniques.

 

Media Formulation Tool

Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.

Support

Cell Culture & Transfection Support Center

Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

 

Need technical support?
Contact our expert team for technical and application support of laboratory products.

Cell Culture Products Designed for Sustainability

Cell culture products designed for sustainability

Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.