KingFisher Apex System

The Thermo Scientific KingFisher Apex represents the pinnacle of automated purification for DNA, RNA, proteins, and cells. Building on decades of KingFisher product value and excellence, we’ve combined exceptional instrument capabilities with complete touchscreen-based control to deliver excellent flexibility and performance. The KingFisher Apex makes sample prep easy.

For current information on the Sample Prep and Molecular Biology IVDR transition updates, please visit the Sample Prep and Molecular Biology

IVDR Transition Updates page.

Explore KingFisher Apex with a self-guided tour

Virtually navigate the KingFisher Apex instrument and get a closer look at the features, supporting kits and reagents, and more!

Take 3D tour

Note: This tool is best viewed in Chrome, Firefox, or Safari browser. Learn more

Features

In addition to the common features of all KingFisher Purification Systems, the Apex offers:

High-throughput purification
1–96 samples per run

Choose from two plate formats
Wide 20 μL–5 mL volume range

Download protocols from our library
Or easily customize protocols using Thermo Scientific BindIx Software

Easy to install and run with simple workflow
Follow on-screen instructions (including color guided plate loading) and start first run in <10 minutes

Security & maintenance features
User login and access levels for added security. Pop-up reminders for yearly maintenance.

Ultraviolet lamp
Decontamination is standard

Elute directly to PCR plates
Store your samples for later use with PCR compatible storage tubes

Touchscreen control

A modern touchscreen display and an intuitive interface puts you in control of instrument operation. Follow the guided visuals for easy plate loading, protocol changes, and instrument setup.

It’s easy to edit or write protocols directly on the instrument, and you can customize protocols at every step—bind, wash, elute. You also have the option to manage protocols on your PC with BindIx software. Instrument-defined limits help reduce potential errors in any protocol modifications.

KingFisher Apex touchscreen interactive demo

Click buttons to see preview

Dual magnets, tip combs, and plates

Dual magnets, multiple formats

When you install two magnets and heating blocks, KingFisher Apex intelligently selects the correct magnetic head based on your protocol. Work from large to small volume ranges by choosing from 24-well, 96-well, or PCR formats with corresponding magnetic heads and plates.

Demo videos

Protocols and software

Thermo Scientific BindIx software is included with KingFisher Apex instruments. The software makes it easy to create, download, run, modify, and store protocols directly on the instrument or on your PC.

Protocols are available for nucleic acid and protein purification procedures with reagent kits. The software allows you to define and edit steps, parameters, plates, and reagents for every part of your protocol—bind, wash, and elute. You can even generate status reports including a run log, plate layout, and step parameters.

For more information about BindIx, see the Features tab.

Attention KingFisher Flex users: KingFisher Flex scripts and protocols can be readily opened and converted to KingFisher Apex scripts.

To download BindIx software, choose one of the methods in the table.

Three ways to download BindIx software

Method	Instructions
Web link	Double-click the downloaded package to start the installation process
Microsoft Store	Click Get, then Install
USB drive from manufacturer	Double-click the .msix bundle file, then click Install

System requirements: Windows 10 version 1809 (17763.0) or higher

Applications

The KingFisher Apex can gently and efficiently isolate and purify nucleic acids, proteins, and cells from a range of sample types for a wide variety of downstream applications. For a selection of application kits, reagents, and protocols that run on the KingFisher Apex, see the Kits, Reagents, and Protocols tab.

KingFisher instruments and Dynabeads magnetic beads

Automated immunoprecipitation with seamless integration to protein analysis

KingFisher Apex instrument with dual magnet heads and upgraded cooling, heating, and elution features

Tips for isolating highly pure exosome subpopulations for western blot

Dynabeads magnetic beads—the key to successful immunoprecipitation

Plates, plastics, and setup

For information about plastics and plate setup products designed to work with the KingFisher Apex, see the Accessories tab.

Specifications

Attribute	Description
Instrument size	Benchtop
Throughput level	Medium to high
Processing volume by plate format	96-well plate Deep-well: 50–1,000 µL KingFisher standard: 15–200 µL PCR: 10–80 µL 24-well plate Deep-well: 30–5,000 µL Storage tubes 96 tubes: 30–200 µL 24 tubes: 200–1,000 µl
Samples per run	24 or 96
Software included	BindIx
Customizable protocols	Yes, created with instrument user interface or BindIx PC software*
Heating/cooling ranges	Heating up to 100ºC Cooling down to 4ºC
Ultraviolet lamps	2 UV lamps, max run time 23 h 59 min
Dimensions (H x W x D)	45 x 78 x 59 cm (18 x 31 x 23 in)
Weight	56 kg (123 lb)
Plate formats	96 deep-well plate 96-well KingFisher standard plate 96-well PCR plate (skirted and semiskirted) 24 deep-well plate 96 storage tubes 24 storage tubes
Instrument memory	~30 GB
Display interface	Graphical user interface with touch screen
Power	100–240 VAC 50/60 Hz, 280 VA
Barcode reader	On instrument; 1D barcodes
Magnetic head options	4 options, customer interchangeable
Data connectivity	USB device for PC USB Wi-Fi or LAN adapter for network RS-232 for automation Cloud enabled

* Requires Windows 10 version 1809 (17763.0) or higher.

Ordering information

Download the KingFisher Apex brochure