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QuantStudio 5 Real-Time PCR System
Take charge of how you create and manage your real-time PCR results
The QuantStudio 5 Real-Time PCR System is ideal for all levels of users who need a high-performance instrument with features for maximum experiment control. With built-in security features, locked workflows, user account management and options for Web browser-based or desktop analysis, the QuantStudio 5 Real-Time PCR System helps connect you to your data more easily than ever before and offers uncompromising performance and quality.
The QuantStudio 5 Real-Time PCR System is available with 96-well thermal blocks with either 0.1 or 0.2 mL well volume or a 384-well thermal block.
Contact us
Telephone: 800 327 3002, option 4, then option 3 (toll-free in the US only)
Telephone 00 800 5345 5345, option 3 (toll-free in Europe, Middle East and South Africa only)
Get started quickly—The factory-calibrated system is delivered in a user-friendly package, enabling quick installation and immediate use.
Skip the learning curve—With preoptimized templates and an intuitive interface that requires minimal training, the QuantStudio Design and Analysis Software is available in desktop and Web browser-based format.
Helps save time with efficient experiment runs—Applied Biosystems™ VeriFlex™ technology offers 3 independent temperature zones for PCR optimization, and fast thermal cycling enables you to get results in as little as 30 minutes.
Results you can trust—Applied Biosystems™ OptiFlex™ technology with white LED and 4 coupled channels enables optimal well-to-well and instrument-to-instrument accuracy.
Get started with a limited budget—Get the modern industrial design and superior functionality you have come to know with the Applied Biosystems™ QuantStudio family of high-performance instruments. The QuantStudio 3 instrument is the perfect entry-level system and allows you to stretch your budget.
Get connected to your data more easily and quickly than ever before, with a real-time PCR system that offers an interactive touch screen for a modern usability experience similar to mobile devices and the option to choose Web browser–based software enabled through the Thermo Fisher Cloud. With the cloud, you can:
- Remotely monitor your runs and access data anytime, anywhere*
- Analyze sophisticated data sets quickly
- Store results in a secure data storage space
- Share and collaborate with colleagues at your institution or around the world
For peace of mind, the Thermo Fisher Cloud offers high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services® (AWS) to offer a robust platform.
*Where the Web is accessible
Utilizing proven Applied Biosystems OptiFlex technology and VeriFlex blocks, the QuantStudio 3 system offers improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.
The QuantStudio 5 system also supports probe-based assays and intercalating dyes. TaqMan probe-based assays enable outstanding specificity and sensitivity. Applied Biosystems SYBR Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 5 system offers 96- well and 384-well format options, allowing for a broader range of detection chemistries and assay multiplexing. The 96-well format has six excitation filters (450–680 nm) and six emission filters (500–730 nm), and the 384-well format has five excitation filters (450–650 nm) and five emission filters (500–700 nm), calibrated form FAM/SYBR Green, VIC, ABY/NED/TAMRA, Jun/ROX dyes, and Cy5/Mustang Purple dyes.
Precise quantification with 1.5-fold discrimination
A
B
Figure 1. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with Applied Biosystems PE2 TaqMan. Assay under Fast run conditions using TaqMan. Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059); 3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard curve generated from the Ct values.
Excellent reproducibility and 10-log dynamic range
Figure 2. Real-time PCR reproducibility. This plot shows results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block. The data show highly reproducible results over 10 logs of input template amount, illustrating the broad linear dynamic range of the system.
Melt curve analysis
Figure 3. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using Applied Biosystems SYBR Select Master Mix with primers for RNase P followed by a dissociation step. The reactions were run under Fast run conditions, showing Ct uniformity with a mean of 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 84.17°C (SD 0.07°C).
Genotyping analysis
Figure 4. Allelic discrimination plot with traces using real-time PCR data. Cluster plot of 44 gDNA samples and 4 no-template controls (NTCs) genotyped using Applied Biosystems TaqMan. SNP Genotyping Assay C_29086771_20, with both PCR and allelic discrimination performed on the QuantStudio 5 Real-Time PCR System. The novel use of real-time PCR data to plot SNP cluster progress aids in calling ambiguous samples and reduces run times by displaying the optimal number of cycles necessary for maximum cluster separation.
Multiplex gene expression
Figure 5. Multiplex reaction with four targets plus passive reference. Whole-plate amplification plots of 96 replicates of cDNA made from universal human RNA (UHR) amplified under Fast run conditions using Applied Biosystems TaqMan. Multiplex Master Mix with Mustang Purple passive reference dye. Targets and labels: FZD1 labeled with FAM dye, APOE labeled with VIC dye, CD44 labeled with ABY dye, GAPDH labeled with JUN dye.
| Repair Center service plans | On-site service plans | |||
|---|---|---|---|---|
| Service plans at a glance | AB Repair Center Support Plus | AB Maintenance Plus | AB Assurance | AB Complete |
| On-site response time | Target 2 business days* | 2 business days* | Next business day* | |
| Scheduled on-site planned maintenance (PM) | Yes | Yes | Yes | |
| Remote diagnostics | Yes | Yes | Yes | Yes |
| Parts, labor, and travel for repair included | Yes | 10% discount | Yes | Yes |
| Computer repair and replacement included | Yes | 10% discount | Yes | Yes |
| Priority access to Tech Support Mon–Fri, 8 a.m.—5 p.m. local time | Yes | Yes | Yes | |
| Priority access to Remote Service Engineer | Yes | Yes | Yes | |
| Requalification post-PM and critical repairs | Yes | |||
| Field Application Scientist consultation | Yes | |||
| Loaner instrument issued during repair (Repair Center plans only) | Yes | |||
*Availability limited in some geographic areas.
QuantStudio Family of Real-Time PCR and Digital PCR Systems
Product brochure: QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems
Your Innovative Research: QuantStudio 5 Real-Time PCR System
Application Note: QuantStudio 3 and 5 Real-Time PCR Systems
Your Innovative Research: Expression of oncogenic microRNAs
Application Note: Protein Thermal Shift Technology
Get started quickly—The factory-calibrated system is delivered in a user-friendly package, enabling quick installation and immediate use.
Skip the learning curve—With preoptimized templates and an intuitive interface that requires minimal training, the QuantStudio Design and Analysis Software is available in desktop and Web browser-based format.
Helps save time with efficient experiment runs—Applied Biosystems™ VeriFlex™ technology offers 3 independent temperature zones for PCR optimization, and fast thermal cycling enables you to get results in as little as 30 minutes.
Results you can trust—Applied Biosystems™ OptiFlex™ technology with white LED and 4 coupled channels enables optimal well-to-well and instrument-to-instrument accuracy.
Get started with a limited budget—Get the modern industrial design and superior functionality you have come to know with the Applied Biosystems™ QuantStudio family of high-performance instruments. The QuantStudio 3 instrument is the perfect entry-level system and allows you to stretch your budget.
Get connected to your data more easily and quickly than ever before, with a real-time PCR system that offers an interactive touch screen for a modern usability experience similar to mobile devices and the option to choose Web browser–based software enabled through the Thermo Fisher Cloud. With the cloud, you can:
- Remotely monitor your runs and access data anytime, anywhere*
- Analyze sophisticated data sets quickly
- Store results in a secure data storage space
- Share and collaborate with colleagues at your institution or around the world
For peace of mind, the Thermo Fisher Cloud offers high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services® (AWS) to offer a robust platform.
*Where the Web is accessible
Utilizing proven Applied Biosystems OptiFlex technology and VeriFlex blocks, the QuantStudio 3 system offers improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.
The QuantStudio 5 system also supports probe-based assays and intercalating dyes. TaqMan probe-based assays enable outstanding specificity and sensitivity. Applied Biosystems SYBR Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 5 system offers 96- well and 384-well format options, allowing for a broader range of detection chemistries and assay multiplexing. The 96-well format has six excitation filters (450–680 nm) and six emission filters (500–730 nm), and the 384-well format has five excitation filters (450–650 nm) and five emission filters (500–700 nm), calibrated form FAM/SYBR Green, VIC, ABY/NED/TAMRA, Jun/ROX dyes, and Cy5/Mustang Purple dyes.
Precise quantification with 1.5-fold discrimination
A
B
Figure 1. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with Applied Biosystems PE2 TaqMan. Assay under Fast run conditions using TaqMan. Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059); 3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard curve generated from the Ct values.
Excellent reproducibility and 10-log dynamic range
Figure 2. Real-time PCR reproducibility. This plot shows results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block. The data show highly reproducible results over 10 logs of input template amount, illustrating the broad linear dynamic range of the system.
Melt curve analysis
Figure 3. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using Applied Biosystems SYBR Select Master Mix with primers for RNase P followed by a dissociation step. The reactions were run under Fast run conditions, showing Ct uniformity with a mean of 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 84.17°C (SD 0.07°C).
Genotyping analysis
Figure 4. Allelic discrimination plot with traces using real-time PCR data. Cluster plot of 44 gDNA samples and 4 no-template controls (NTCs) genotyped using Applied Biosystems TaqMan. SNP Genotyping Assay C_29086771_20, with both PCR and allelic discrimination performed on the QuantStudio 5 Real-Time PCR System. The novel use of real-time PCR data to plot SNP cluster progress aids in calling ambiguous samples and reduces run times by displaying the optimal number of cycles necessary for maximum cluster separation.
Multiplex gene expression
Figure 5. Multiplex reaction with four targets plus passive reference. Whole-plate amplification plots of 96 replicates of cDNA made from universal human RNA (UHR) amplified under Fast run conditions using Applied Biosystems TaqMan. Multiplex Master Mix with Mustang Purple passive reference dye. Targets and labels: FZD1 labeled with FAM dye, APOE labeled with VIC dye, CD44 labeled with ABY dye, GAPDH labeled with JUN dye.
| Repair Center service plans | On-site service plans | |||
|---|---|---|---|---|
| Service plans at a glance | AB Repair Center Support Plus | AB Maintenance Plus | AB Assurance | AB Complete |
| On-site response time | Target 2 business days* | 2 business days* | Next business day* | |
| Scheduled on-site planned maintenance (PM) | Yes | Yes | Yes | |
| Remote diagnostics | Yes | Yes | Yes | Yes |
| Parts, labor, and travel for repair included | Yes | 10% discount | Yes | Yes |
| Computer repair and replacement included | Yes | 10% discount | Yes | Yes |
| Priority access to Tech Support Mon–Fri, 8 a.m.—5 p.m. local time | Yes | Yes | Yes | |
| Priority access to Remote Service Engineer | Yes | Yes | Yes | |
| Requalification post-PM and critical repairs | Yes | |||
| Field Application Scientist consultation | Yes | |||
| Loaner instrument issued during repair (Repair Center plans only) | Yes | |||
*Availability limited in some geographic areas.
QuantStudio Family of Real-Time PCR and Digital PCR Systems
Product brochure: QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems
Your Innovative Research: QuantStudio 5 Real-Time PCR System
Application Note: QuantStudio 3 and 5 Real-Time PCR Systems
Your Innovative Research: Expression of oncogenic microRNAs
Application Note: Protein Thermal Shift Technology
Resources
Support
For Research Use Only. Not for use in diagnostic procedures.