Ion GeneStudio S5 System

Expand your research to cover 2M to 130M sequencing reads per run with this scalable platform

The Ion GeneStudio S5 systems are designed to enable a broad range of targeted next-generation sequencing (NGS) applications with speed and scalability. Five Ion S5 chips enable a sequencing throughput range of 2M to 130M reads per run.

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Sequencing and analysis in as little as three hours

As little as 15 minutes hands-on time and same day results.

Sequencing and analysis in as little as three hours

 

Ion GeneStudio S5 System

Ion GeneStudio S5 Plus System

Ion GeneStudio S5 Prime System

Max. throughput/day (chip type)

15 Gb
(one Ion 540 Chip)

30 Gb
(two Ion 540 chips)

50 Gb
(two Ion 550 chips)

Total sequencing and analysis time at max. throughput

19 hr (Ion 540 Chip)

10 hr (Ion 540 Chip)
11.5 hr (Ion 550 Chip)

6.5 hr (Ion 540 Chip)
8.5 hr (Ion 550 Chip)

Compatible chips

Ion 510, 520, 530, 540 chips

Ion 510, 520, 530, 540, and 550 chips

 Download specification sheet ›

Sequencing throughput to support multiple applications of NGS

From gene panels to exomes, gene expression profiling to transcriptomes, or microbial genomes to microbiomes, Ion Torrent semiconductor sequencing optimizes cost and throughput for small and large NGS projects.

Cancer research:
gene panels for SNPs, indels, copy number, gene expression, and gene fusion analysis

Inherited disease research:
panels for targeted gene or whole exome analysis

Gene expression analysis:
whole transcriptome RNA-Seq, targeted RNA sequencing, and small RNA sequencing

Reproductive health research:
aneuploidy detection, carrier screening research
 Microbiology/infectious disease research:
microbial whole genomes, microbial typing, and metagenomics		 

Ion S5 Sequencer and Ion S5 XL Sequencer are DNA sequencing devices enable DNA analysis by measuring hydrogen ions released during nucleotide bindings via semi-conductor chips. This device is used to analyze DNA sequences at various specific regions among multiple human genes.

(Classification: DNA Sequencer, Reg. #: 15-4212, Product Name: Ion S5 Sequencer / Ion S5 XL Sequencer)
"These products are registered medical devices in Korea. Please make sure to read through user guide and precautions carefully before use."

Parts/Components

#Product NameProduct No.DescriptionSpecifications
1

Ion Server
(S5 XL Component)

A28563

An external device used for data analysis after sequencing step.
Executes primary analysis.

-100-240VAC, 50/60Hz, 6.7A
-Dimensions:
56.6cm(H)x56.6cm(L)x21.6cm(W)
※ Compatible with above
specifications or higher

2Ion Chef Instrument4484178

An automated sample preparation system.
A library DNA is attached to ISP, amplified and enriched to conduct sequencing in next step.

-100-240VAC, 50/60Hz, 12A
-Dimension:
56.1cm(H)x 70.0cm(D)x 71.4cm(W)
-Weight: 68.2kg

3Ion 520 Chip KitA27762

Semi-conductor chips loaded with DNA samples.
Approximately 10 million wells/chip

-Storage temperature: 15~30℃
-Approx. 10 million wells / 1 chip
- 8 chips / 1 kit

4Ion 530 Chip KitA27763

Semi-conductor chips loaded with DNA samples.
Approximately 40 million wells/chip

-Storage temperature: 15~30℃
-Approx. 40 million wells / 1 chip
- 8 chips / 1 kit

5Ion 540 Chip KitA27766

Semi-conductor chips loaded with DNA samples.
Approximately 160 million wells/chip

-Storage temperature: 15~30℃
-Approx. 160 million wells / 1 chip
-8 chips / 1 kit


Cancer research

Enabling NGS analysis and discovery of multi-biomarker types (fusions, insertion/deletions (indels), single nucleotide variants, and copy number variations), Oncomine assays are part of an end-to-end workflow that includes simple, scalable sequencing with optimized bioinformatics and reporting—designed for cancer research.

Logistic regression scores for responders and non-responders

ROC for prediction of response by TCR convergence and evenness

zhang-fong-convergence

Figure 1. Prediction of immunotherapy outcome via TCR evenness and convergence

Peripheral blood TCRB repertoire convergence and clonal expansion predict response to anti-CTLA-4 monotherapy for cancer

Approach: Leverage the low substitution error rate of the Ion Torrent platform to evaluate convergence as a predictive biomarker for response to anti-CTLA-4 monotherapy in a set of 22 individuals with cancer. Eight peripheral blood leukocyte (PBL) samples derived from three donors were analyzed using the Oncomine TCRB-LR assay or Sequenta/Adaptive Biotechnologies TCRB assay.

Results: The combination of TCR evenness and convergence improves prediction of response (AUC = .89).

Download the poster


Complex and inherited disease research

Our complete NGS solutions are uniquely suited to research in understanding how the combination of genetics and the environment influence development of complex diseases such as autoimmune disorders, neurodegenerative diseases, and many others.

Inherited-disease

Molecular analysis of inherited cardiomyopathy using next generation semiconductor sequencing technologies

Approach: identify causative variants of different inherited cardiomyopathies using a single Ion AmpliSeq gene panel.

Results: Potential pathogenic variants found in 23.6% of study samples including a homozygous variant in the SLC25A4 gene.

Read the publication 

Targeted-pnaels

Video: Targeted panels or exome—which is the right NGS approach for inherited disease research?


Microbiology/infectious disease research

Uncover microbial diversity, study pathogen outbreaks, and identify mutations that may be associated with antibiotic resistance. Take advantage of increased throughput, higher accuracy, and longer reads to produce rapid and accurate sequencing of microbes with streamlined sample preparation and a simple, scalable, and optimized data analysis workflow.

A time travel story: metagenomic analyses decipher the unknown geographical shift and the storage history of possibly smuggled antique marble statues

Approach: reconstruct the history of the storage of three single statues to infer the relationship among them, and to elucidate their geographical shift using metagenomic analyses of the 16S ribosomal DNA (rDNA) and other analyses

Results: eukaryotic microbiomes showed similarities comprising many soil-inhabiting organisms. Two statues displayed many halotolerant and halophilic bacteria which may indicate a longer stay in arid and semi-arid surroundings as well as marine environments

Read the publication 

Metagenomic-analyses


Ion Torrent targeted next-generation sequencing workflow

prep-sequence-analyze
  • From as little as 1 ng DNA or RNA input material, prep libraries using FFPE, fine needle aspirates, cell-free DNA, and other sample types using Ion AmpliSeq technology
  • Automate library prep, template prep, and chip loading with Ion Chef System automation
  • 2.5-hr sequencing runs on Ion GeneStudio S5 instruments with <15 minutes of setup time using simple cartridge-loaded reagents
  • Transform data to insight with analysis, QC reporting, and variant calling on Connect cloud-based platform or an in-house Ion Reporter Server
Step 1 : Library and template preparation
prep-sequence-analyze
  • From as little as 1 ng DNA or RNA input material, prep libraries using FFPE, fine needle aspirates, cell-free DNA, and other sample types using Ion AmpliSeq technology
  • Automate library prep, template prep, and chip loading with Ion Chef System automation
Step 2 : Sequencing
  • 2.5-hr sequencing runs on Ion GeneStudio S5 instruments with <15 minutes of setup time using simple cartridge-loaded reagents
Step 3 : Data analysis
  • Transform data to insight with analysis, QC reporting, and variant calling on Connect cloud-based platform or an in-house Ion Reporter Server

Support

  • Get expert support from a global network of over 1,300 service and support personnel dedicated to helping you maximize data integrity and instrument investment

Targeted Gene Sequencing Resources

For Research Use Only. Not for use in diagnostic procedures.