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HPLC에서 UHPLC로 분석법을 변경하길 원하신다면 이 온라인 툴을 사용하세요. 이 툴은 새로운 그라디언트 테이블을 계산하고 주입량, 유량 및 실행 시간을 조정합니다. 현재의 분석법 조건을 입력하고 UHPLC를 사용하면 얼마나 많은 시간, 시료 및 용매를 절약할 수 있는지 확인해 보세요.

모든 책임은 사용자에게 있습니다. 미리 입력된 데이터는 추정치일 뿐입니다. 써모 피셔 사이언티픽은 데이터, 계산 또는 결과의 정확성에 대한 책임을 지지 않습니다.

Use at your own risk. Prepopulated data are estimates only. Thermo Fisher Scientific takes no responsibility for the accuracy of data, calculations or results.

Current Method

Current Column

Length (mm):

Diameter (mm):

Particle Size (µm):

UHPLC Method

Planned Column

Length (mm):

Diameter (mm):

Particle Size (µm):

Peak Details (Critical Pair)

Actual R_s: (Resolution Factor)

Peak Details (Critical Pair)

Predicted R_s Change Factor:

Predicted R_s:

Current Method Conditions

Flow (mL/min):

Injection Volume (µL):

Max Observed Pressure:

Pressure Units:

Number of Samples:

Data Collection Rate (Hz):

Gradient Delay Vol:

Gradient Delay Volume (µL):

Recommended Method Conditions

* Boost Factor:

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

Flow (mL/min):

Injection Volume (µL):

Estimated Max Observed Pressure:

0 bar

Number of Samples:

Data Collection Rate (Hz):

0 Hz

Gradient Delay Volume (µL):

Optimal GDV (µL):

Adjust Flow:

Current Gradient Table

Step	Time (min)	%A
1		100.0
2
3
4
5
6
7
8
9
10
End Time:	0.000
Recommended Reconditioning Time: n.a.

Planned Gradient Table

Time (min)	%A	%B	%C	%D
0.00	100.0









End Time:	0.000
Recommended Reconditioning Time: n.a.

Sample usage savings

Current Usage	Planned Usage	Savings

Eluent usage and time savings

	Current Total	Planned Total	Savings	Throughput
Eluent Usage
Time

HPLC Method Transfer Calculator

Current Method

UHPLC Method

Current Gradient Table

Planned Gradient Table

Sample usage savings

Eluent usage and time savings

Total Time Savings

Total Eluent Savings

Total Sample Usage Savings