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The Attune NxT Autosamplers
*image shown with option accessories
Efficient. Flexible. Transformative.
Now you are able to choose the option that best suits your throughput and experimental needs. Based on the proven performance of the Attune NxT Autosampler, the CytKick Autosampler and CytKick Max Autosampler models are designed to offer walk-away automation seamlessly integrated with your Attune NxT Flow Cytometer for increased productivity and added choice. All Attune NxT Autosampler models offer the following:
- Broad compatibility—compatible with many different plate formats, including 96-well, 384-well, and deep-well plates (up to 2 mL) and 1.5 mL and 2.0 mL tube racks – up to 24 tubes per vessel (CytKick Max Autosampler)
- Intelligent probe—intelligent probe design minimizes clogging and carryover (0.5%) and prevents damage to the instrument
- Auto cleaning—performs automated cleaning when the instrument is shutting down
- Consistent data—designed to provide minimal variation within a plate
- Mixing method—aspiration and stirring depending upon well geometry and acquisition volume mixing sample by aspiration instead of shaking ensures homogeneity of the sample and maintains cell viability. See why mixing sample by aspiration is better
- One-click transition—no disassembly, no additional QC, no reboot required for conversion between plates and tubes
- Sample Cooling—passive cooling available in 96-well U-bottom plates and microcentrifuge tube racks
Attune NxT Autosampler options for high throughput
Attune NxT Autosampler
- Basic model option
- Throughput—<45 min for 96-well plate using high-throughput mode
- Sampling—small dead volume of 30 µL for 12.5–200 µL/min, 50 µL for 50–1,000 µL/min
- Compatibility—96 deep-well, 96-well standard depth, 384 deep-well, 384-well standard depth
CytKick Autosampler
- Standard model option
- Throughput—<42 min for 96-well plate using high-throughput mode
- Sampling—small dead volume of 30 µL for 12.5–200 µL/min, 50 µL for 500 µL/min, 1,000 µL/min
- Compatibility—96 deep-well, 96-well standard depth, 384 deep-well, 384-well standard depth
CytKick Max Autosampler
- Optimal model option
- High-throughput mode—22 min for 96-well plate (boost mode, using one rinse and one mix and full analysis of a 20 µL sample)
- Sample cooling—Passive cooling available for 96-well U-bottom plates and microcentrifuge tube racks
- Maximum compatibility—96 deep-well, 96-well standard depth, 384 deep-well, 384-well standard depth, 1.5 mL and 2 mL microcentrifuge tube rack (up to 24 per rack), 96-well foil covered, 384-well foil covered, and is customizable to accept additional plate types
Specifications
 |  |  | |
| Specification | Attune NxT Autosampler | CytKick Autosampler | CytKick Max Autosampler |
|---|---|---|---|
| High-throughput mode acquisition time | <42 minutes for 96-well plate, using high-throughput mode (using one rinse and one mix and full analysis of a 40 µL sample) | <42 minutes for 96-well plate, using high-throughput mode (using one rinse and one mix and full analysis of a 20 µL sample) | 22 min for 96-well plate (boost mode) (using one rinse and one mix and full analysis of a 20 µL sample) |
| Carryover | <0.5% in plate loader format (standard mode, 2 wash cycles); multiple-rinse capability for ultra-low carryover |
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|
| Sample mixing | Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume | Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume | Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume; gentle mix mode for fragile cells |
| Mixing method | Each well mixed via aspiration of sample (no shaking) | Each well mixed via aspiration and dispensation of sample (no shaking) | Each well mixed via aspiration and stirring depending upon well geometry and acquisition volume |
| Wash cycle | User-defined number of wash cycles (up to 10 wash cycles) | User-defined number of wash cycles (up to 10 wash cycles) | User-defined number of wash cycles (up to 10 wash cycles) |
| Minimum dead volume (single draw) | 30 μL for 12.5–200 μL/min, 50 μL for 1,000 μL/min | 30 µL for 12.5-200 µL/min, 50 μL for 500 μL/min, 1,000 μL/min | 30 µL for 12.5-200 µL/min, 50 μL for 500 μL/min, 1,000 μL/min |
| Sample window | Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition | Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition | Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition |
| Auto-calibration | Regular, 30-day interval, system-initiated function | Regular, 30-day interval, system-initiated function | Regular, 30-day interval, system-initiated function |
| Plate and tube compatibility | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes |
| Compatible plate types |
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|
|
| Cleaning cycles | Automated daily and monthly cleaning protocols | Automated daily and monthly cleaning protocols | Automated daily and monthly cleaning protocols |
| Fluidics requirements |
| Total fluid volume: 2.0 L external tanks | Total fluid volume: 2.0 L external tanks |
| Extended fluidics | Available with External Fluid Supply (EFS)—optional external fluid tank with 10 L fluid capacity | Available with External Fluid Supply (EFS) —optional external fluid tank with 10 L fluid capacity | Available with External Fluid Supply (EFS) —optional external fluid tank with 10 L fluid capacity |
| Size (W x D x H) | ~29 x 29 x 40 cm (11 x 11 x 16 in.) | ~43 x 33 x 41 cm (17 x 13 x 16 in.) | ~43 x 33 x 41 cm (17 x 13 x 16 in.) |
| Space requirements |
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| Mounting | Flanks to Attune NxT Flow Cytometer | Flanks to Attune NxT Flow Cytometer | Flanks to Attune NxT Flow Cytometer |
| Weight | ~16 kg (35 lb) |
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|
Straightforward run set-up
Setting up a multiwell plate sampling run using the Attune NxT software is simple. Using a virtual plate layout view, you can create compensation wells, define instrument settings, and identify samples. The software also allows you to define multiple experiments on a single plate and recover unused sample (for samples collected or acquired in tube mode only).
The intuitive software is easy to use and enables quick data analysis. For example, a heat-map view allows rapid screening and confirmation of samples while the instrument is acquiring data.
Attune NxT Autosampler heat map function identifies variation within a parameter across a 96-well plate. Live and heat-killed THP-1 cells were stained with propidium iodide and dispensed into a 96-well plate. Samples were applied to the Attune NxT flow cytometer using the Attune Nxt Autosampler. Heat map (indigo correlating with live cells and red with dead cells) and numeric overlay text provide real-time feedback on the assay being performed.
Reliable utility for diverse applications
Automation helps maximize reproducibility between experiments and reduces inter-assay variability and inter-operator variability. By demonstrating low CVs across and through plates, the Attune NxT Flow Cytometer with Autosampler offers a reliable and high-throughput approach for multiparametric cell screening with 96- and 384-well plates, allowing for less sample quantities to maximize the efficient use of a limited numbers of cells. This combination of higher-throughput with higher-content flow cytometric analysis of as many as 16 parameters for each cell with acquisition speeds of up to 1 mL/min provides a powerful screening platform for drug discovery and systems biology experiments. See some examples of applications enabled by Attune NxT Flow Cytometer with Autosampler below.
Flow Cytometry Analysis of Dose Response for Apoptosis Induction
Live/Dead Algal Analysis With the Attune NxT Flow Cytometer
High-Throughput Automation With the Attune NxT Autosampler: Consistent Results Across All Wells and Across Plates
Consistent results regardless of sampling method
The Attune NxT Flow Cytometer Automation configuration with the Attune NxT CytKick Max Autosampler demonstrates consistent results regardless of whether the sample is delivered from a tube or from a plate.
Fixed Jurkat cells run in a 96-well U-bottom plate compared to the same sample run in tube format. CV is a well to well comparison of event count. Tube to plate comparison is percent difference.
| Experiment | CV % |
|---|---|
| Tubes before CV | 1.62% |
| Tubes after CV | 2.98% |
| Plate CV | 1.65% |
| % diff, plate to tube | 0.84% |
Extended fluidics option
The Attune NxT Flow Cytometer Automation Configuration is available with the Attune NxT External Fluid Supply (EFS), with 10 L fluid capacity to support extended runtimes. The EFS supplements the on-board fluidics of the Attune NxT Flow Cytometer.
Attune NxT External Fluid Supply (EFS) offers:
- CytKick Max Universal Cooling Adaptor (Cat. No. 100081492)
- Thermo Scientific Orbitor Rs2, Microplate Mover:
- Thermo Scientific Cytomat 2 C4xx-LiN Automated Incubator Series (Cat. No. 51033033)
Fluids consumables
- Attune Focusing Fluid, 1X (Cat. No. 4488621): Buffered, azide-free support/carrier reagent (sterile) containing surfactant optimized for transporting particles.
- Attune Wash Solution (Cat. No. A24974): Formulated to remove cellular debris, prevent protein building up, and minimize dyes sticking to the fluidics lines of the system.
- Attune Shutdown Solution, 1X (Cat. No. A24975): Formulated to minimize bubble formulation that can occur in the system lines overnight, to help keep the fluid lines clean, and to minimize microbial growth in the fluid lines.
Superior service and support
Extended coverage service plans are available at the time of instrument purchase. With our service plans, you’ll maximize system uptime, reduce overall repair costs, get fast repair turnaround time from a manufacturer-trained and certified field service engineer (FSE), extend the life of your instrument, and help keep it running at peak performance. Choose from a variety of service options that balance your budget, productivity, uptime, and regulatory requirements. Plans start with the most basic repair models and scale to premium offerings, including advanced support and compliance services. To build your personalized service quote, go to thermofisher.com/serviceselector.
Service plans at a glance
| Attune NxT Flow Cytometer, CytKick Autosampler & CytKick Max Autosampler | Attune NxT Autosampler, CytKick Autosampler & CytKick Max Autosampler | |
|---|---|---|
| AB Assurance | Rapid Exchange | |
| Standard on-site response time | 2 business days* | Replacement shipped out in 1 business day |
| Priority access to technical support (Monday–Friday, standard business hours) | ||
| Scheduled on-site PM | Optional add-on | |
| Parts, labor, and travel for repair | Off-site repair service including shipping, parts, and labor | |
| Remote instrument diagnostics | ||
| Priority access to remote service engineer |
* Availability limited in some geographic areas.
Remote monitoring and diagnostics service
The Smart Monitor tool is a real-time, remote instrument performance monitoring service that incorporates an instrument management dashboard, remote diagnostics, and aggregated performance data tracking. [Note that Smart Monitor service is subject to internet connectivity.]
Training
We offer a combination of virtual and in-person classroom instruction, and hands-on learning in your lab to match your schedule, budget, and learning preferences. Whichever course style you choose, you’ll learn from one of our 300 highly skilled application scientists who are available to lead sessions online, at your location, or at one of our 12 training centers located worldwide.
Attune NxT Autosampler options for high throughput
Attune NxT Autosampler
- Basic model option
- Throughput—<45 min for 96-well plate using high-throughput mode
- Sampling—small dead volume of 30 µL for 12.5–200 µL/min, 50 µL for 50–1,000 µL/min
- Compatibility—96 deep-well, 96-well standard depth, 384 deep-well, 384-well standard depth
CytKick Autosampler
- Standard model option
- Throughput—<42 min for 96-well plate using high-throughput mode
- Sampling—small dead volume of 30 µL for 12.5–200 µL/min, 50 µL for 500 µL/min, 1,000 µL/min
- Compatibility—96 deep-well, 96-well standard depth, 384 deep-well, 384-well standard depth
CytKick Max Autosampler
- Optimal model option
- High-throughput mode—22 min for 96-well plate (boost mode, using one rinse and one mix and full analysis of a 20 µL sample)
- Sample cooling—Passive cooling available for 96-well U-bottom plates and microcentrifuge tube racks
- Maximum compatibility—96 deep-well, 96-well standard depth, 384 deep-well, 384-well standard depth, 1.5 mL and 2 mL microcentrifuge tube rack (up to 24 per rack), 96-well foil covered, 384-well foil covered, and is customizable to accept additional plate types
Specifications
| | | |
| Specification | Attune NxT Autosampler | CytKick Autosampler | CytKick Max Autosampler |
|---|---|---|---|
| High-throughput mode acquisition time | <42 minutes for 96-well plate, using high-throughput mode (using one rinse and one mix and full analysis of a 40 µL sample) | <42 minutes for 96-well plate, using high-throughput mode (using one rinse and one mix and full analysis of a 20 µL sample) | 22 min for 96-well plate (boost mode) (using one rinse and one mix and full analysis of a 20 µL sample) |
| Carryover | <0.5% in plate loader format (standard mode, 2 wash cycles); multiple-rinse capability for ultra-low carryover |
|
|
| Sample mixing | Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume | Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume | Mixing optimized to preserve cell viability; mixing cycles optimized to sample analysis volume; gentle mix mode for fragile cells |
| Mixing method | Each well mixed via aspiration of sample (no shaking) | Each well mixed via aspiration and dispensation of sample (no shaking) | Each well mixed via aspiration and stirring depending upon well geometry and acquisition volume |
| Wash cycle | User-defined number of wash cycles (up to 10 wash cycles) | User-defined number of wash cycles (up to 10 wash cycles) | User-defined number of wash cycles (up to 10 wash cycles) |
| Minimum dead volume (single draw) | 30 μL for 12.5–200 μL/min, 50 μL for 1,000 μL/min | 30 µL for 12.5-200 µL/min, 50 μL for 500 μL/min, 1,000 μL/min | 30 µL for 12.5-200 µL/min, 50 μL for 500 μL/min, 1,000 μL/min |
| Sample window | Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition | Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition | Protectively coated window allows visibility to well progress while preventing exposure to ambient light during acquisition |
| Auto-calibration | Regular, 30-day interval, system-initiated function | Regular, 30-day interval, system-initiated function | Regular, 30-day interval, system-initiated function |
| Plate and tube compatibility | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes |
| Compatible plate types |
|
|
|
| Cleaning cycles | Automated daily and monthly cleaning protocols | Automated daily and monthly cleaning protocols | Automated daily and monthly cleaning protocols |
| Fluidics requirements |
| Total fluid volume: 2.0 L external tanks | Total fluid volume: 2.0 L external tanks |
| Extended fluidics | Available with External Fluid Supply (EFS)—optional external fluid tank with 10 L fluid capacity | Available with External Fluid Supply (EFS) —optional external fluid tank with 10 L fluid capacity | Available with External Fluid Supply (EFS) —optional external fluid tank with 10 L fluid capacity |
| Size (W x D x H) | ~29 x 29 x 40 cm (11 x 11 x 16 in.) | ~43 x 33 x 41 cm (17 x 13 x 16 in.) | ~43 x 33 x 41 cm (17 x 13 x 16 in.) |
| Space requirements |
|
|
|
| Mounting | Flanks to Attune NxT Flow Cytometer | Flanks to Attune NxT Flow Cytometer | Flanks to Attune NxT Flow Cytometer |
| Weight | ~16 kg (35 lb) |
|
|
Straightforward run set-up
Setting up a multiwell plate sampling run using the Attune NxT software is simple. Using a virtual plate layout view, you can create compensation wells, define instrument settings, and identify samples. The software also allows you to define multiple experiments on a single plate and recover unused sample (for samples collected or acquired in tube mode only).
The intuitive software is easy to use and enables quick data analysis. For example, a heat-map view allows rapid screening and confirmation of samples while the instrument is acquiring data.
Attune NxT Autosampler heat map function identifies variation within a parameter across a 96-well plate. Live and heat-killed THP-1 cells were stained with propidium iodide and dispensed into a 96-well plate. Samples were applied to the Attune NxT flow cytometer using the Attune Nxt Autosampler. Heat map (indigo correlating with live cells and red with dead cells) and numeric overlay text provide real-time feedback on the assay being performed.
Reliable utility for diverse applications
Automation helps maximize reproducibility between experiments and reduces inter-assay variability and inter-operator variability. By demonstrating low CVs across and through plates, the Attune NxT Flow Cytometer with Autosampler offers a reliable and high-throughput approach for multiparametric cell screening with 96- and 384-well plates, allowing for less sample quantities to maximize the efficient use of a limited numbers of cells. This combination of higher-throughput with higher-content flow cytometric analysis of as many as 16 parameters for each cell with acquisition speeds of up to 1 mL/min provides a powerful screening platform for drug discovery and systems biology experiments. See some examples of applications enabled by Attune NxT Flow Cytometer with Autosampler below.
Flow Cytometry Analysis of Dose Response for Apoptosis Induction
Live/Dead Algal Analysis With the Attune NxT Flow Cytometer
High-Throughput Automation With the Attune NxT Autosampler: Consistent Results Across All Wells and Across Plates
Consistent results regardless of sampling method
The Attune NxT Flow Cytometer Automation configuration with the Attune NxT CytKick Max Autosampler demonstrates consistent results regardless of whether the sample is delivered from a tube or from a plate.
Fixed Jurkat cells run in a 96-well U-bottom plate compared to the same sample run in tube format. CV is a well to well comparison of event count. Tube to plate comparison is percent difference.
| Experiment | CV % |
|---|---|
| Tubes before CV | 1.62% |
| Tubes after CV | 2.98% |
| Plate CV | 1.65% |
| % diff, plate to tube | 0.84% |
Extended fluidics option
The Attune NxT Flow Cytometer Automation Configuration is available with the Attune NxT External Fluid Supply (EFS), with 10 L fluid capacity to support extended runtimes. The EFS supplements the on-board fluidics of the Attune NxT Flow Cytometer.
Attune NxT External Fluid Supply (EFS) offers:
- CytKick Max Universal Cooling Adaptor (Cat. No. 100081492)
- Thermo Scientific Orbitor Rs2, Microplate Mover:
- Thermo Scientific Cytomat 2 C4xx-LiN Automated Incubator Series (Cat. No. 51033033)
Fluids consumables
- Attune Focusing Fluid, 1X (Cat. No. 4488621): Buffered, azide-free support/carrier reagent (sterile) containing surfactant optimized for transporting particles.
- Attune Wash Solution (Cat. No. A24974): Formulated to remove cellular debris, prevent protein building up, and minimize dyes sticking to the fluidics lines of the system.
- Attune Shutdown Solution, 1X (Cat. No. A24975): Formulated to minimize bubble formulation that can occur in the system lines overnight, to help keep the fluid lines clean, and to minimize microbial growth in the fluid lines.
Superior service and support
Extended coverage service plans are available at the time of instrument purchase. With our service plans, you’ll maximize system uptime, reduce overall repair costs, get fast repair turnaround time from a manufacturer-trained and certified field service engineer (FSE), extend the life of your instrument, and help keep it running at peak performance. Choose from a variety of service options that balance your budget, productivity, uptime, and regulatory requirements. Plans start with the most basic repair models and scale to premium offerings, including advanced support and compliance services. To build your personalized service quote, go to thermofisher.com/serviceselector.
Service plans at a glance
| Attune NxT Flow Cytometer, CytKick Autosampler & CytKick Max Autosampler | Attune NxT Autosampler, CytKick Autosampler & CytKick Max Autosampler | |
|---|---|---|
| AB Assurance | Rapid Exchange | |
| Standard on-site response time | 2 business days* | Replacement shipped out in 1 business day |
| Priority access to technical support (Monday–Friday, standard business hours) | ||
| Scheduled on-site PM | Optional add-on | |
| Parts, labor, and travel for repair | Off-site repair service including shipping, parts, and labor | |
| Remote instrument diagnostics | ||
| Priority access to remote service engineer |
* Availability limited in some geographic areas.
Remote monitoring and diagnostics service
The Smart Monitor tool is a real-time, remote instrument performance monitoring service that incorporates an instrument management dashboard, remote diagnostics, and aggregated performance data tracking. [Note that Smart Monitor service is subject to internet connectivity.]
Training
We offer a combination of virtual and in-person classroom instruction, and hands-on learning in your lab to match your schedule, budget, and learning preferences. Whichever course style you choose, you’ll learn from one of our 300 highly skilled application scientists who are available to lead sessions online, at your location, or at one of our 12 training centers located worldwide.
3D Demo: Explore the Attune NxT Flow Cytometer and Autosampler
A software tool for exploring the hardware, software, optics and fluidics of the Attune NxT Flow Cytometer. Hot spots throughout the tool feature data, researcher opinions, and technical specifications. Mouse operation enables rotation, zoom, and measurement. Available in 8 languages.
Design your flow panels using the Invitrogen Flow Cytometer Panel Builder with advanced functionality.
thermofisher.com/flowpanel
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