Technical Resources - Media Formulations

NEUROBASAL™ Medium (1X) liquid

Neurobasal™ Media are basal media formulated to meet the neuronal cells special requirements.

They allow for long-term maintenance of the normal phenotype and growth of neuronal cells, and maintain pure populations of neuronal cells without the need of an astrocyte feeder layer.

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.030.00.4
L-Alanine89.02.00.02247191
L-Arginine hydrochloride211.084.00.39810428
L-Asparagine-H2O150.00.830.0055333334
L-Cysteine121.031.50.2603306
L-Histidine hydrochloride-H2O210.042.00.2
L-Isoleucine131.0105.00.8015267
L-Leucine131.0105.00.8015267
L-Lysine hydrochloride183.0146.00.7978142
L-Methionine149.030.00.20134228
L-Phenylalanine165.066.00.4
L-Proline115.07.760.06747826
L-Serine105.042.00.4
L-Threonine119.095.00.79831934
L-Tryptophan204.016.00.078431375
L-Tyrosine181.072.00.39779004
L-Valine117.094.00.8034188
Vitamins
Choline chloride140.04.00.028571429
D-Calcium pantothenate477.04.00.008385744
Folic Acid441.04.00.009070295
Niacinamide122.04.00.032786883
Pyridoxal hydrochloride204.04.00.019607844
Riboflavin376.00.40.0010638298
Thiamine hydrochloride337.04.00.011869436
Vitamin B121355.00.00685.0184503E-6
i-Inositol180.07.20.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0200.01.8018018
Ferric Nitrate (Fe(NO3)3"9H2O)404.00.12.4752476E-4
Magnesium Chloride (anhydrous)95.077.30.8136842
Potassium Chloride (KCl)75.0400.05.3333335
Sodium Bicarbonate (NaHCO3)84.02200.026.190475
Sodium Chloride (NaCl)58.03000.051.724136
Sodium Phosphate monobasic (NaH2PO4-H2O)138.0125.00.9057971
Zinc sulfate (ZnSO4-7H2O)288.00.1946.736111E-4
Other Components
D-Glucose (Dextrose)180.04500.025.0
HEPES238.02600.010.92437
Phenol Red376.48.10.021519661
Sodium Pyruvate110.025.00.22727273

Reference:

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