Technical Resources - Media Formulations

11320 - DMEM/F-12

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.018.750.25
L-Alanine89.04.450.049999997
L-Arginine hydrochloride211.0147.50.69905216
L-Asparagine-H2O150.07.50.05
L-Aspartic acid133.06.650.05
L-Cysteine hydrochloride-H2O176.017.560.09977272
L-Cystine 2HCl313.031.290.09996805
L-Glutamic Acid147.07.350.05
L-Glutamine146.0365.02.5
L-Histidine hydrochloride-H2O210.031.480.14990476
L-Isoleucine131.054.470.41580153
L-Leucine131.059.050.45076334
L-Lysine hydrochloride183.091.250.4986339
L-Methionine149.017.240.11570469
L-Phenylalanine165.035.480.2150303
L-Proline115.017.250.15
L-Serine105.026.250.25
L-Threonine119.053.450.44915968
L-Tryptophan204.09.020.04421569
L-Tyrosine disodium salt dihydrate261.055.790.21375479
L-Valine117.052.850.4517094
Vitamins
Biotin244.00.00351.4344263E-5
Choline chloride140.08.980.06414285
D-Calcium pantothenate477.02.240.0046960167
Folic Acid441.02.650.0060090707
Niacinamide122.02.020.016557377
Pyridoxine hydrochloride206.02.0130.009771844
Riboflavin376.00.2195.824468E-4
Thiamine hydrochloride337.02.170.0064391694
Vitamin B121355.00.685.0184503E-4
i-Inositol180.012.60.07
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0116.61.0504504
Cupric sulfate (CuSO4-5H2O)250.00.00135.2E-6
Ferric Nitrate (Fe(NO3)3"9H2O)404.00.051.2376238E-4
Ferric sulfate (FeSO4-7H2O)278.00.4170.0015
Magnesium Chloride (anhydrous)95.028.640.30147368
Magnesium Sulfate (MgSO4) (anhyd.)120.048.840.407
Potassium Chloride (KCl)75.0311.84.1573334
Sodium Bicarbonate (NaHCO3)84.02438.029.02381
Sodium Chloride (NaCl)58.06995.5120.61207
Sodium Phosphate dibasic (Na2HPO4) anhydrous142.071.020.50014085
Sodium Phosphate monobasic (NaH2PO4-H2O)138.062.50.45289856
Zinc sulfate (ZnSO4-7H2O)288.00.4320.0015
Other Components
D-Glucose (Dextrose)180.03151.017.505556
Hypoxanthine Na159.02.390.015031448
Linoleic Acid280.00.0421.4999999E-4
Lipoic Acid206.00.1055.097087E-4
Phenol Red376.48.10.021519661
Putrescine 2HCl161.00.0815.031056E-4
Sodium Pyruvate110.055.00.5
Thymidine242.00.3650.0015082645

Reference:

1. Dulbecco, R. and Freeman, G., (1959) Plaque formation by the polyoma virus. Virology 8:396.

2. Ham, R.G., (1965) Clonal growth of mammalian cells in a chemically defined synthetic medium. Proc. Natl. Acad. Sci., 53:288.

3. Morton, H.J. (1970) A survey of commercially available tissue culture media. In Vitro 6(2):89.

4. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:185.

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