Attaining near-atomic resolution with the Glacios 2 Cryo-TEM using E-CFEG

Duration: 20 minutes

A powerful new feature is boosting resolution at 200 kV

The Thermo Scientific Glacios 2 Cryo-TEM is a high-throughput, high-resolution 200 kV platform that allows you to easily collect near-atomic data from a broad range of biological targets. With the latest addition of an optional low-energy-spread cold field emission gun (E-CFEG), resolution can be improved to 1.5 Å (Apoferritin).

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This advanced microscope makes cryo-EM more accessible and is ideally suited for single-particle analysis, cryo-electron tomography (cryo-ET), and micro-electron diffraction (MicroED) applications.

 

Watch our virtual event to learn how combining the Glacios 2 Cryo-TEM with E-CFEG boosts resolution to near atomic levels.

 

In this webinar, you will learn:

  • How advances in resolution with E-CFEG on a 200 kV microscope can provide deeper insights
  • The various applications the Glacios 2 Cryo-TEM can support
  • How the Glacios 2 Cryo-TEM can help you bring cryo-EM capabilities into your institution

About the speakers

Jeff Lengyel, Director of Product Marketing, Thermo Fisher Scientific

In his current role at Thermo Fisher, Jeff focuses on electron microscopy for life sciences applications. His previous roles include Americas principal scientist for cryo-EM, product marketing manager for structural biology, onsite team lead for the FEI/NIH Living Lab for Structural Biology, and senior Titan Krios Cryo-TEM applications specialist. Jeff received his Ph.D. in biochemistry from the University of Cambridge (UK) as a University of Cambridge/National Institutes of Health health sciences scholar. Under this scholarship he was co-mentored by Professor Richard Perham of Cambridge and Dr. Sriram Subramaniam of the NIH. For his doctoral research, Jeff studied the pyruvate dehydrogenase using a combination of cryo-EM and biophysical techniques.


Lingbo Yu, Product Marketing Manager, Thermo Fisher Scientific

Lingbo received her PhD from the University of Vermont working on algorithms specifically designed for low-dose cryo-EM images of biological samples in the lab of Michael Radermacher. She also worked on the FEI/NIH Living Lab project as a software developer, optimizing automated single particle data collection. Later, she moved to the Netherlands and joined the Thermo Fisher Scientific team.  


Michael Adams, Application Scientist, Thermo Fisher Scientific

Michael received a bachelor of science in biology from the University of Alabama at Birmingham. He then pursued a master’s degree in biological sciences from the University of Konstanz in Germany, where he entered the field of structural biology through X-ray crystallography. Michael then earned his PhD in structural biology from EMBL Grenoble, where he characterized the structure and function of important effector proteins in the pathogenic bacterium Legionella, which helped him familiarize himself with cryo-EM. Michael now works for Thermo Fisher as an application scientist at the NanoPort in Eindhoven, specializing in life sciences transmission electron microscopy.