This webinar presents a new approach to glycoform fingerprinting that uses proton-transfer charge-reduction with gas-phase fractionation to analyze intact glycoproteins on the Thermo Scientific Orbitrap Ascend MS platform. The method provides a detailed landscape of the intact molecular weights present in biotherapeutic protein preparations in a single experiment and offers insights into glycoform composition when coupled with a suitable bioinformatic strategy.

In addition, we highlight the application of charge detection native mass spectrometry (Thermo Scientific Direct Mass Technology mode available on the Thermo Scientific Q Exactive UHMR MS) towards the elucidation of masses and aggregation of heterogeneous glycosylated biotherapeutics.

About the presenter

Wendy Sandoval, Director, Translational Mass Spectrometry, Genentech

Wendy Sandoval, Director, Translational Mass Spectrometry, Genentech

Wendy Sandoval and her team focus on analytical methods to characterize new biotherapeutic modalities including native and top-down mass spectrometry, resolving protein interactions, and lipidomics to support early research and clinical projects. Wendy has co-authored over 100 manuscripts and two books.

 
Register to view the free on-demand webinar

* Required field

Style Sheet for Global Design System
CMD Wide-format style fixes