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It is sometimes possible to stain cells after fixation but this is dependent upon the effects of fixation on the epitope of your protein-of-interest. The list below contains information about the performance of different clones after fixation using our Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set (cat. no. 88-8824) or after IC Fixation Buffer (cat. no. 00-8222) followed by treatment with methanol. Please note that staining after fixation may allow for intracellular stores of protein to also be stained with the antibody.
Antigen | Clone | Live cells (before IC fixation)1 | After IC fixation and perm wash1 | After IC fixation/ methanol2 |
---|---|---|---|---|
HUMAN | ||||
CD3 | OKT3 | +++ | ++ | + |
UCHT1 | +++ | +++ | +++ | |
SK7 | +++ | ++ | +++ | |
CD4 | OKT4 | +++ | + | +/- |
RPA-T4 | +++ | +++ | + | |
SK3 | +++ | +++ | + | |
CD5 | UCHT2 | +++ | +++ | +++ |
CD8 | OKT8 | +++ | +/- | - |
RPA-T8 | +++ | +++ | +/- | |
SK1 | +++ | +++ | +/- | |
CD8b | SIDI8BEE | +++ | ++ | ++ |
CD11a | HI111 | +++ | +++ | + |
CD11b | CBRM1/5 | + | - | +++ |
ICRF44 | ++ | - | ++ | |
CD11c | 3.9 | ++ | + | ++ |
CD14 | 61D3 | +++ | +++ | ++ |
CD19 | HIB19 | +++ | ++ | +/- |
CD20 | 2H7 | +++ | +++ | - |
CD25 | BC96 | + | - | +/- |
CD27 | O323 | +++ | + | + |
CD31 | WM59 | +++ | ++ | +++ |
CD33 | p67.6 | +++ | +++ | +++ |
CD38 | HB7 | ++ | - | ++ |
HIT2 | ++ | - | ++ | |
CD40 | 5C3 | ++ | - | ++ |
CD44 | IM7 | +++ | +++ | +++ |
CD45 | 2D1 | +++ | +++ | +++ |
HI30 | +++ | +++ | +++ | |
CD45RA | HI100 | +++ | +++ | +++ |
CD45RO | UCHL1 | +++ | +++ | ++ |
CD56 | MEM-188 | + | - | +/- |
CB56 | + | - | +/- | |
CMSSB | ++ | +/- | - | |
CD57 | TB01 | ++ | ++ | ++ |
CD62L | DREG-56 | ++ | - | ++ |
CD69 | FN50 | +++ | ++ | ++ |
CD80 | 2D10.4 | + | - | + |
CD83 | HB15e | ++ | ++ | ++ |
CD86 | IT2.2 | + | + | + |
CD94 | HP-3D9 | ++ | ++ | ++ |
CD95 | DX2 | + | - | + |
CD127 | RDR5 | ++ | + | ++ |
CD161 | HP-3G10 | ++ | ++ | + |
MOUSE | ||||
CD3 | 145-2C11 | ++ | + | +/- |
500A2 | +++ | +++ | +++ | |
17A2 | +++ | +++ | +++ | |
CD4 | GK1.5 | +++ | ++ | +++ |
RM4-5 | +++ | +++ | +++ | |
CD8 | 53-6.7 | +++ | ++ | ++ |
CD11b | M1/70 | ++ | ++ | ++ |
CD11c | N418 | +++ | +++ | ++ |
CD19 | 1D3 | +++ | ++ | - |
MB19-1 | ++ | - | - | |
CD24 | M1/69 | +++ | +++ | ++ |
CD25 | PC61.5 | + | - | + |
3C7 | ++ | - | + | |
7D4 | ++ | ++ | + | |
CD39 | 24DMS1 | + | - | ++ |
CD44 | IM7 | ++ | ++ | ++ |
CD45 | 30-F11 | +++ | +++ | +++ |
CD45R(B220) | RA3-6B2 | +++ | ++ | +++ |
CD49b | DX5 | ++ | - | + |
CD69 | H1.2F3 | ++ | - | - |
CD185(CXCR5) | SPRCL5 | ++ | - | - |
GR-1 | RB6-8C5 | +++ | +++ | ++ |
NK1.1 | PK136 | ++ | + | + |
IgD | 11-26c | +++ | +++* | ++ |
IgM | ll/41 | ++ | ++ | ++ |
TCR beta | H57-597 | ++ | ++ | ++ |
RAT | ||||
CD25 | OX39 | + | - | |
CANINE | ||||
CD4 | ykix302.9 | +++ | + | |
CD5 | ykix322.3 | +++ | - | |
CD8a | ycate55.9 | +++ | ++ | |
CD44 | ykix337.8 | +++ | ++ | |
CD45 | ykix716.13 | +++ | ++ | |
CD45R | ykix753.22 | +++ | ++ | |
CD90 | ykix337.217 | +++ | ++ | |
MHC Class II | ykix334.2 | +++ | +++ |
*Please note the clones with * indicate longer fixations are detrimental to staining.
For Research Use Only. Not for use in diagnostic procedures.