U-2 OS cells were transduced with Premo™ Autophagy sensor LC3B-RFP (red) and CellLight® Tubulin-GFP (green)

U-2 OS cells were transduced with Premo™ Autophagy sensor LC3B-RFP (red) and CellLight® Tubulin-GFP (green) and cultured overnight. The following day cells were treated with 60uM Chloroquine and cultured overnight. Cells were then counterstained with 1ug/mL Hoeschst 33342 (blue) and imaged on a Delta Vision wide field microscope.

U-2 OS cells were transduced with Premo™ Autophagy sensor LC3B-RFP (red) and CellLight® Tubulin-GFP (green) and cultured overnight. The following day cells were treated with 60uM Chloroquine and cultured overnight. Cells were then counterstained with 1ug/mL Hoeschst 33342 (blue) and imaged on a Delta Vision wide field microscope.

Related Products

Related Images

Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›

Endothelial cells. Go ›

1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›

Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›

REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›

BPAE cells fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit. Go ›

Live cell imaging with CellLight™ reagents. Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›

Live cells transduced with Organelle Lights™ or Cellular Lights™ reagents. Go ›

Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›

Using anti-LC3B and the Click-iT® technology autophagy detected and glycoproteins labeled in HeLa cells Go ›