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Images from confocal laser-scanning microscope optical cross-sectioning of our 15 µm FocalCheck™ microspheres that have a dark red–fluorescent ring stain with a green-fluorescent stain throughout the bead (Cat. no. F7239). The left panel provides a clear visual representation of poor instrument alignment. Correct image registration has been achieved in the right panel, where the dark red ring is aligned with the green disk. The image was contributed by Paulette Brunner, Keck Imaging Center, University of Washington, and Yu-Zhong Zhang, Life Technologies.