Oncomine Myeloid MRD Assays (RUO)

The Ion Torrent Oncomine Myeloid MRD assays (RUO) on the Ion GeneStudio S5 System offer a complete next-generation sequencing (NGS) solution for myeloid MRD detection. Highly sensitive NGS, based on Ion AmpliSeq HD technology, which enables a limit of detection as low as 0.05% allele frequency.

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Poster: Analytical performance of a novel next-generation sequencing assay for myeloid MRD

A DNA and an RNA assay are available to cover a wide range of myeloid MRD targets, including key single nucleotide variants (SNVs), insertions and deletions (indels), tandem duplications, and key gene fusions. The carefully curated targets on the panel are relevant for all major types of myeloid neoplasms, including acute myeloid leukemias (AML), myelodysplastic syndromes (MDS), and myeloproliferative neoplasms (MPN) samples.

Key features:

Comprehensive panels—a DNA and an RNA assay that helps you assess a wide range of variants associated myeloid MRD, including SNVs, indels, gene fusions, and tandem duplications
Fast and simple workflow—using the Ion GeneStudio S5 system, have results in 2–3 days with only ~2 hours of hands-on time required
High sensitivity—error correcting NGS with Ion AmpliSeq HD technology helps provide a low limit of detection (down to 0.05% allele frequency) required for MRD analysis
Optional chimerism analysis—an optional micro-haplotye panel enables sensitive assessment of donor-recipient mixtures with a limit of detection (LOD) as low as 0.2% AF
Integrated informatics—an analytically validated informatics pipeline and reporting solution that includes longitudinal tracking helps simplify the interpretation of results

Customer webinar

Genomic profiling from initial assessment to detection of MRD: How NGS can rapidly deliver key insights for myeloid neoplasms

Hear Dr. Bevan Tandon review ongoing research in his lab using the Oncomine Myeloid Assay GX and share recent analytical data using the Oncomine Myeloid MRD assays for highly sensitive MRD detection.

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Gene content summary

DNA assay	RNA assay
33 genes, including 2 full genes and FLT3-ITDs	42 fusion driver genes
1,255 hotspots	990 unique fusions
Optional 22-amplicon micro-haplotype panel for chimerism analysis	6 genes with exon splicing variants
	5 expression control genes

Gene list

DNA assay				RNA assay
Target genes				Fusion driver genes					Expression controls	Exon splicing variants
ABL1	FLT3**	NRAS	TP53*	ABL1	ETV6		NTRK3	RET	ABL1	KMT2A
ASXL1	GATA2	PHF6	U2AF1	ABL2	FGFR1	MECOM	NUP214	RUNX1	GUSB	RUNX1
BCOR	IDH1	PTPN11	WT1	ALK	FUS	MLLT10	NUP98	TAL1	PSMB2	NOTCH1
BRAF	IDH2	RUNX1		BCL2	GLIS2	MRTFA (MKL1)	PAX5	TCF3	PUM1	ETV6
CALR	JAK2	SETBP1		BRAF	HMGA2	MYBL1	PDGFRA	TCF4	TRIM27	IKZF1
CBL	KIT	SF3B1		CCND1	JAK2	MYH11	PDGFRB	TFE3		NTRK1
CEBPA*	KRAS	SH2B3		CREBBP	KAT6A (MOZ)	NOTCH1	RARA	ZNF384
CSF3R	MPL	SRSF2		CSF1R	KAT6B	NTRK1	RARB
DNMT3A	MYD88	STAG2		EGFR	KMT2A (includes PTD)	NTRK2	RARG
EZH2	NPM1	TET2