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Multiplex NGS based genotyping
Our portfolio of One Lambda AllType assays offers a complete next generation sequencing solution that provides single tube amplification for all eleven Class I and Class II loci, completely removing the need for amplicon pooling. Samples are transferred directly to library preparation and made ready for sequencing in a single workday. Sequencing analysis and individual allele assignments are automatically generated by our One Lambda TypeStream Visual NGS Analysis Software. Results are delivered in as little as three days.
Multiplex PCR for genotyping with NGS offers a simple, fast, and better workflow
Many labs might find NGS difficult to adopt due to the increased complexity, dedicated labor requirements and data management challenges. Our NGS assays are designed for simplicity and flexibility allowing laboratories greater cross-training on their platform of choice.
Platform-specific barcoding allows for sequencing on the Illumina MiSeq, MiniSeq, iSeq 100, and Ion Torrent Ion GeneStudio S5 sequencing systems.
Features and benefits
Next Generation Sequencing (NGS) is a powerful technology offering high resolution typing with greater sample throughput. Nonetheless, many labs find it difficult to adopt due to the increased complexity, dedicated labor requirements and data management challenges.
Designed for simplicity and efficiency, our AllType FASTplex NGS Assay makes NGS accessible and achievable for any laboratory. The assay’s advanced chemistry and comprehensive coverage is complemented by a streamlined workflow for minimal hands-on time and supported by intuitive software for easy sample auditing and enhanced data integration.
Next-Generation Sequencing with the AllType FASTplex NGS Assay
NGS is a powerful but complex technology genotyping. An intelligently simple approach, the One Lambda AllType FASTplex NGS Assay is the only easy, single-test solution supported by streamlined and integrated software.
Why choose the AllType FASTplex NGS solution?
- One multiplex PCR reaction per sample
- Early sample pooling
- Total hands-on time <90 minutes
- Automated data transfer and analysis
AllType FASTplex – quality with less complexity
The AllType FASTplex chemistry has been enhanced to improve performance, robustness, and reliability. With the newly added Class II exon 1 primer mix, the AllType FASTplex reagents reduce ambiguities, provide comprehensive coverage across 11 loci, and generate highly concordant results at 99.8% or higher, all in a single PCR reaction.
Figure 1. AllType FASTplex exon coverage.
Workflow simplicity and reduced hands-on time
The assay can enhance your lab’s efficiency with reduced labor, pipetting, and risk of contamination. By combining fragmentation and barcoding steps into a single reaction, the workflow allows for samples to be pooled early in the protocol so that library preparation can be completed in a single tube.
The protocol has been designed for simplicity, easy implementation, and greater cross-training in the lab. From start to finish, the workflow can be performed in under 7 hours with less than 90 minutes of hands-on time.
Figure 2. AllType FASTplex workflow.
Choose the right NGS assay, protocol, and coverage for your lab
Our AllType and AllType FASTplex NGS assays apply a single multiplex reaction to amplify all major Class I and Class II loci, completely removing the need for amplicon pooling. Both provide scalable solutions for low- and high-volume laboratories.
| AllType FASTplex NGS | AllType NGS | ||
| Method | Multiplex long-range amplification | Multiplex long-range amplification | |
| Turnaround time | 1-2 days
|
2-3 days | |
| Complexity | Medium (does not require specialized training of lab personnel) |
High | |
| Resolution | Ultra-high | Ultra-high | |
| Throughput | High | High | |
| Regulatory status | RUO and IVD Refer to products for details |
RUO and IVD Refer to products for details |
|
| Instruments | Illumina MiSeq, MiniSeq, iSeq 100, Ion GeneStudio S5, and Ion GeneStudio S5 plus | Illumina MiSeq, MiniSeq, iSeq 100, Ion GeneStudio S5, and Ion GeneStudio S5 plus | |
* Considers one technologist preparing 24 samples.
Software
Streamlined next generation sequencing solution that's flexible
When the workflow is complete, our TypeStream Visual NGS Analysis Software will begin the automatic analysis of your data while you’re away from your desk. The streamlined interface enables easy sample auditing, bi-directional communication with One Lambda HistoTrac Software as well as data migration into our One Lambda HLA Fusion Software for advanced analytics, such as antibody tracking, crossmatching, and epitope analysis.
Platform-specific barcoding allows for sequencing on the Illumina MiSeq, MiniSeq, iSeq 100, Ion Chef, and Ion GeneStudio S5 sequencing systems.
Intuitive data integration
TypeStream Visual software now includes bi-directional communication with HistoTrac, enabling the seamless transfer of results between both systems for better data management, reduced transcription errors, and optimized reporting.
Your results can also be combined in our HLA Fusion Software for advanced analytics, such as antibody tracking, crossmatching, and epitope analysis.
Resources
Not all products are CE marked of have 510(k) clearance for sale in the US. Availability of product in each country depends on local regulatory marketing authorization status. MiSeq, MiniSeq, and iSeq are trademarks of Illumina, Inc. © 2024 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Not all products are available in all countries. Please consult your local sales representative for details.