Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
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High Tumor Mutational Burden (TMB) has shown association with benefit from immune checkpoint blockade therapies. While TMB as computed from whole exome sequencing (WES) is still a gold standard, the high input material (tumor and germline DNA) requirement and complex bioinformatics refrains exploring this biomarker in individual labs. This study describes the development of a PCR-based targeted panel for computing TMB and detecting important variants from FFPE research samples.