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96-Well Cell Culture Plates
Select 96-well cell culture plates by surface
Whether culturing individual cell lines or scaling up for high-throughput screening, there is a Thermo Scientific Nunc 96-well plate for your needs. Advances in surface modifications, well geometry and optical clarity mean a variety of 96-well cell culture plate tailored for your specific application, including optical bottom plates for cellular imaging.
To help ensure reliability, flexibility, and reproducibility across every stage of cell culture, plates are available with a variety of surfaces optimized for different needs in cell attachment and cell growth.
NEW Nunclon Supra Surfaces for advanced cell culturing
We are proud to provide a new product line surface treatment that will support improved cell yield and morphology as well enabling you to do xeno-free or low-serum applications.
Order yours today: Dishes | Flasks | Plates | 96-well plates
See how our surfaces perform with 39 cell types and cell lines at thermofisher.com/supradata
Non-treated surface for suspension culture and general biochemical assays
Made from high-quality USP Class VI virgin polystyrene, solid Nunc 96-well plates (in white and black) are ideal for colorimetric, fluorescence-, and luminescence-based cell analysis as well as protein binding assays
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack size | Case size | |
|---|---|---|---|---|---|---|---|---|
| 269620 | Nunc 96-Well Flat Bottom | Non-treated | Clear | No | No | Sleeve of 5 | 60 | Order |
| 260860 | Nunc 96-Well Flat Bottom | Non-treated | Clear | Yes | Yes | Sleeve of 10 | 160 | Order |
| 266120 | Nunc 96-Well Flat Bottom | Non-treated | Clear | Yes | Yes | Sleeve of 25 | 100 | Order |
| 243656 | Nunc 96-Well Flat Bottom | Non-treated | Clear | Yes | Yes | Sleeve of 6 | 108 | Order |
| 269787 | Nunc 96-Well Flat Bottom | Non-treated | Clear | No | Yes | Individually packed | 50 | Order |
| 260895 | Nunc 96-Well Flat Bottom | Non-treated | Clear | Yes | No | Sleeve of 10 | 160 | Order |
| 260844 | Nunc 96-Well Flat Bottom, Barcoded | Non-treated | Clear | No | No | Sleeve of 10 | 180 | Order |
| 260887 | Nunc 96-Well Flat Bottom, Barcoded | Non-treated | Clear | Yes | Yes | Sleeve of 10 | 160 | Order |
| 260836 | Nunc 96-Well Flat Bottom | Non-treated | Clear | No | No | Sleeve of 10 | 180 | Order |
| Sleeve of 10 |
| Sleeve of 10 |
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack Size | Case Size | |
|---|---|---|---|---|---|---|---|---|
| 268152 | Nunc 96-Well U Bottom | Non-treated | Clear | No | No | Sleeve of 10 | 180 | Order |
| 268200 | Nunc 96-Well U Bottom | Non-treated | Clear | Yes | Yes | Sleeve of 10 | 160 | Order |
| 262162 | Nunc 96-Well U Bottom | Non-treated | Clear | No | Yes | Individually packed | 50 | Order |
| 278606 | Nunc 96 Deep Well U Bottom | Non-treated | Clear | No | Yes | Sleeve of 4 | 32 | Order |
| 278605 | Nunc 96 Deep Well U Bottom | Non-treated | Clear | No | No | Sleeve of 4 | 32 | Order |
| 262146 | Nunc 96-Well U-Bottom | Non-treated | Clear | No | No | Bulk pack of 135 | 135 | Order |
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack Size | Case Size | |
|---|---|---|---|---|---|---|---|---|
| 249570 | Nunc 96-Well V Bottom | Non-treated | Clear | No | No | Sleeve of 10 | 180 | Order |
| 249662 | Nunc 96-Well V Bottom | Non-treated | Clear | No | Yes | Individually packed | 50 | Order |
| 249952 | Nunc 96-Well V Bottom | Non-treated | Clear | Yes | Yes | Sleeve of 10 | 160 | Order |
| 249935 | Nunc 96-Well V Bottom | Non-treated | Clear | Yes | Yes | Sleeve of 10 | 80 | Order |
| 249940 | Nunc 96-Well V Bottom | Non-treated | Clear | No | Yes | Sleeve of 10 | 80 | Order |
Nunc 96-well cell culture treated plates for adherent cells
The Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. Our Nunclon Delta products are subjected to rigorous quality control procedures to meet the standards of Thermo Fisher Scientific and to ensure lot-to-lot consistency.
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack size | Case size | |
|---|---|---|---|---|---|---|---|---|
| 167008 | Nunc 96-Well Flat Bottom | Nunclon Delta | Clear | Yes | Yes | Individually packed | 50 | Order |
| 161093 | Nunc 96-Well Flat Bottom | Nunclon Delta | Clear | Yes | Yes | Sleeve of 10 | 160 | Order |
| 136101 | Nunc 96-Well Flat Bottom | Nunclon Delta | White | Yes | Yes | Individually packed | 50 | Order |
| 136102 | Nunc 96-Well Flat Bottom | Nunclon Delta | White | Yes | Yes | Sleeve of 10 | 160 | Order |
| 137101 | Nunc 96-Well Flat Bottom | Nunclon Delta | Black | Yes | Yes | Individually packed | 50 | Order |
| 137103 | Nunc 96-Well Flat Bottom | Nunclon Delta | Black | Yes | Yes | Sleeve of 10 | 160 | Order |
| 168055 | Nunc 96-Well Flat Bottom | Nunclon Delta | Clear | No | Yes | Individually packed | 50 | Order |
Nunclon Sphera surface-treated plates for ultra-low binding surface
The Nunclon Sphera surface-treated plates offer a physiologically relevant 3D cell culture format for disease modeling and drug screening applications in cancer and stem cell research, designed to enable the production of reproducible spheroids that can be further differentiated into specific organoids.
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack size | Case size | |
|---|---|---|---|---|---|---|---|---|
| 174925 | Nunclon Sphera 96-Well U Bottom | Nunclon Sphera | Clear | Yes | Yes | Individually packed | 8 | Order |
| 174929 | Nunclon Sphera 96-Well U Bottom | Nunclon Sphera | Clear | Yes | Yes | Sleeve of 5 | 50 | Order |
| 174927 | Nunclon Sphera 96-Well Flat Bottom | Nunclon Sphera | Clear | Yes | Yes | Individually packed | 8 | Order |
Nunc 96-well Supra treated plates for adherent cells
Thermo Scientific Nunc 96-well plates with Supra surface treatment are designed for culturing finicky adherent cells (primary and MSCs). When results matter most, Supra surface is designed to deliver higher cell yield, reduced time-to-adherence, and better morphology.
Nunclon Supra surface is more hydrophilic than the Nunclon Delta surface, enhancing the growth of finicky cell types under no-serum (xeno-free culture) or low-serum conditions.
Nunclon UpCell surface-treated plates for trypsin free dissociation
The Nunc UpCell surface-treated plates are grafted with PIPAAm polymers that change conformation and hydrophilicity according to external temperature, dislodging cells from the growth surface in this process while keeping cellular membrane and surface receptors intact.
Nunc ECM treated plates for finicky cells
Nunc poly-D-lysine (PDL)- and collagen I-coated plates are designed to enhance the adhesion and growth of primary cells and finicky cell lines in vitro. The PDL synthetic material and the collagen I protein of animal origin are aseptically coated in either the Nunc regular plates or the Nunc Optical Bottom plates with 10-3 sterility assurance level (SAL).
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack size | Case size | |
|---|---|---|---|---|---|---|---|---|
| 152038 | Nunc 96-Well Flat Bottom | Collagen I | Clear | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152039 | Nunc 96-Well Flat Bottom | Poly-D-Lysine | Clear | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152036 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Collagen I | Black | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152040 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Collagen I | White | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152037 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Poly-D-Lysine | Black | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152028 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Poly-D-Lysine | White | Yes | Yes | Sleeve of 5 | 20 | Order |
Nunc Edge 2.0 96-well plate with moat
Thermo Scientific Nunc Edge 2.0 96-well cell culture plates contain a large evaporation moat that can be filled with sterile water or media to help eliminate edge effect.
This edge effect is caused when evaporation occurs in the perimeter wells of a microplate during prolonged incubation. It changes media pH, osmolality, and the concentration of the media constituents, affecting cell viability and function.
However, the built-in moat in the Nunc Edge 2.0 plate significantly reduces evaporation compared to the conventional 96-well plates after four-day incubation, improving well-to-well consistency.
To prevent evaporation, simply:
Utilize all 96 wells
Fill surrounding moat with 2 mL
sterile water or media in each section
Watch as evaporation happens in the moat and not the outer wells
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack size | Case size | |
|---|---|---|---|---|---|---|---|---|
| 167425 | Nunc Edge 2.0 96-Well Plate Treated | Nunclon Delta | Clear | Yes | Yes | Individually packed | 50 | Order |
| 167542 | Nunc Edge 2.0 96-Well Plate Treated | Nunclon Delta | Clear | Yes | Yes | Sleeve of 10 | 160 | Order |
| 167574 | Nunc Edge 2.0 96-Well Plate Treated | Nunclon Delta | Clear | No | Yes | Individually packed | 50 | Order |
| 167554 | Nunc Edge 2.0 96-Well Plate Treated | Nunclon Delta | Clear | No | Yes | Sleeve of 10 | 160 | Order |
| 267427 | Nunc Edge 2.0 96-Well Plate Non-treated | Non-treated | Clear | Yes | Yes | Individually packed | 50 | Order |
| 267544 | Nunc Edge 2.0 96-Well Plate Non-treated | Non-treated | Clear | Yes | Yes | Sleeve of 10 | 160 | Order |
| 267576 | Nunc Edge 2.0 96-Well Plate Non-treated | Non-treated | Clear | No | Yes | Individually packed | 50 | Order |
| 267556 | Nunc Edge 2.0 96-Well Plate Non-treated | Non-treated | Clear | No | Yes | Sleeve of 10 | 160 | Order |
| 267566 | Nunc Edge 2.0 96-Well Plate Non-treated | Non-treated | Clear | No | No | Sleeve of 10 | 160 | Order |
| 267578 | Nunc Edge 2.0 96-Well Plate Non-treated | Non-treated | Clear | Yes | No | Sleeve of 10 | 160 | Order |
| Sleeve of 10 |
| Sleeve of 10 |
96-well plate with optical bottom
Optical bottom plates for cellular imaging
With superior imaging quality and minimal background noise and crosstalk between wells, Nunc optical bottom plates are optimized for fluorescence and luminescence imaging applications.
Optical polystyrene polymer bottom, 96-well plates
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack size | Case size | |
|---|---|---|---|---|---|---|---|---|
| 165305 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Nunclon Delta | Black | Yes | Yes | Sleeve of 10 | 30 | Order |
| 165306 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Nunclon Delta | White | Yes | Yes | Sleeve of 10 | 30 | Order |
| 152036 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Collagen I | Black | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152040 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Collagen I | White | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152037 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Poly-D-lysine | Black | Yes | Yes | Sleeve of 5 | 20 | Order |
| 152028 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Poly-D-lysine | White | Yes | Yes | Sleeve of 5 | 20 | Order |
| 265301 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Non-treated | Black | No | No | Sleeve of 10 | 30 | Order |
| 265302 | Nunc 96-Well Optical Polystyrene Polymer Bottom | Non-treated | White | No | No | Sleeve of 10 | 30 | Order |
Optical coverglass bottom, 96-well plates
| Cat. No. | Description | Surface | Color | Lid | Sterile | Pack size | Case size | |
|---|---|---|---|---|---|---|---|---|
| 164588 | Nunc 96-Well Optical 1.5 Coverglass Bottom | Cell culture-treated | Black | Yes | Yes | Sleeve of 6 | 30 | Order |
| 164590 | Nunc 96-Well Optical 1.5 Coverglass Bottom | Cell culture-treated | White | Yes | Yes | Sleeve of 6 | 30 | Order |
| 160376 | Nunc 96-Well Optical 1.5 CC2 Coverglass Bottom | CC2 enhanced cell culture | Black | Yes | Yes | Sleeve of 6 | 30 | Order |
| 265300 | Nunc 96-Well Optical 1.5 Coverglass Bottom | Non-treated | Black | No | No | Sleeve of 5 | 30 | Order |