Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Targeted resequencing is the comparative analysis of selected regions in a genome to identify genetic variants when compared to a reference. Increasingly targeted resequencing experiments are used in basic and clinical research as scientists look for causative variants for rare and complex diseases within populations.
Exome sequencing is best performed using the TargetSeq™ Exome Enrichment System kits, which are based on an in-solution hybridization method for exome capture. The kits are optimally designed to seamlessly integrate into the 5500 Series Genetic Analysis Systems workflow.
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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples? Learn More: |
Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process. SequalPrep™ Long PCR kit and Normalization plate Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities. Learn More: |
Learn More:
User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub. |
The tools you need for each step in the targeted sequencing data analysis workflow:
Data Analysis Step | Applied Biosystems Software | 3rd-Party Software*** |
---|---|---|
1. Align reads to reference in color space |
| |
2. Generate quality metrics and perform mate pair rescue |
| |
3. Generate sequencing and alignment statistics |
| |
4. Identify Polymorphisms |
| |
5. Translate color space to base space | ||
6. Visualize in context of annotation |
| |
7. Convert to SRF for publishing | ||
*** Need XSQ --> csfasta/qual converter |
Learn more
Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.
BigDye® Direct Cycle Sequencing Kit
Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples? Learn More: |
Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process. SequalPrep™ Long PCR kit and Normalization plate Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities. Learn More: |
Learn More:
User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub. |
The tools you need for each step in the targeted sequencing data analysis workflow:
Data Analysis Step | Applied Biosystems Software | 3rd-Party Software*** |
---|---|---|
1. Align reads to reference in color space |
| |
2. Generate quality metrics and perform mate pair rescue |
| |
3. Generate sequencing and alignment statistics |
| |
4. Identify Polymorphisms |
| |
5. Translate color space to base space | ||
6. Visualize in context of annotation |
| |
7. Convert to SRF for publishing | ||
*** Need XSQ --> csfasta/qual converter |
Learn more
Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.
BigDye® Direct Cycle Sequencing Kit
Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples? Learn More: |
Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process. SequalPrep™ Long PCR kit and Normalization plate Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities. Learn More: |
Learn More:
User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub. |
The tools you need for each step in the targeted sequencing data analysis workflow:
Data Analysis Step | Applied Biosystems Software | 3rd-Party Software*** |
---|---|---|
1. Align reads to reference in color space |
| |
2. Generate quality metrics and perform mate pair rescue |
| |
3. Generate sequencing and alignment statistics |
| |
4. Identify Polymorphisms |
| |
5. Translate color space to base space | ||
6. Visualize in context of annotation |
| |
7. Convert to SRF for publishing | ||
*** Need XSQ --> csfasta/qual converter |
Learn more
Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.
BigDye® Direct Cycle Sequencing Kit
Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples? Learn More: |
Enrich your DNA using one of the following methods: PCR, long-range PCR, array hybridization, in-solution hybridization, or chromosome sorting. After enrichment, consider multiplexing strategies if you have multiple samples to process. SequalPrep™ Long PCR kit and Normalization plate Complete long PCR enrichment and amplicon normalization system that provides a highly efficient enrichment solution to meet NGS re-sequencing throughput capabilities. Learn More: |
Learn More:
User documentation for the 5500 Series Genetic Analysis Systems can be found on the SOLiD Community. Access is through the 5500 Series User Hub. Access is limited to 5500 Series Genetic Analysis Systems users who are registered on the solid community and have been authenticated through the process of requesting permission to join the 5500 Series User Hub. |
The tools you need for each step in the targeted sequencing data analysis workflow:
Data Analysis Step | Applied Biosystems Software | 3rd-Party Software*** |
---|---|---|
1. Align reads to reference in color space |
| |
2. Generate quality metrics and perform mate pair rescue |
| |
3. Generate sequencing and alignment statistics |
| |
4. Identify Polymorphisms |
| |
5. Translate color space to base space | ||
6. Visualize in context of annotation |
| |
7. Convert to SRF for publishing | ||
*** Need XSQ --> csfasta/qual converter |
Learn more
Validate results using our BigDye® Direct Cycle Sequencing Kit, for Sanger sequencing on our capillary electrophoresis-based DNA sequencers.
BigDye® Direct Cycle Sequencing Kit