Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator.

Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator (M36008). Live 3T3 cells were treated with FeTCPP, a superoxide scavenger, (bottom) or left untreated (top). Cells were then labeled with MitoSOX™ Red reagent, which fluoresces when oxidized by superoxide, and nuclei were stained with blue-fluorescent Hoechst 33342. The mitochondria of untreated cells exhibited red fluorescence, indicating the presence of superoxide, whereas the mitochondria of treated cells showed minimal fluorescence.

Detection of superoxide in live cells using MitoSOX™ Red superoxide indicator (M36008). Live 3T3 cells were treated with FeTCPP, a superoxide scavenger, (bottom) or left untreated (top). Cells were then labeled with MitoSOX™ Red reagent, which fluoresces when oxidized by superoxide, and nuclei were stained with blue-fluorescent Hoechst 33342. The mitochondria of untreated cells exhibited red fluorescence, indicating the presence of superoxide, whereas the mitochondria of treated cells showed minimal fluorescence.

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