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Template Preparation

How much oil and recovery solution is typically consumed during a normal Ion Torrent™ Ion OneTouch™ 2 run and during a cleaning?

In a typical Ion OneTouch 2 run, you should expect ~18 mm drop in the level of oil in the tube (~22 mL consumed), and ~13 mm drop in the recovery solution (~15 mL consumed). During cleaning, you should expect to collect ~10 mL of oil in the 50 mL conical waste collection tube.

What should I do if an "Over Pressure" error appears after I start an Ion OneTouch 2 run?

The pressure inside the instrument may be above normal. If the Ion OneTouch 2 firmware is older than v540, please update it to the latest version.

What are these black precipitates in the recovery tube and waste of the Ion OneTouch System?

It may be rubber from the packing. The amount varies among instruments and it is not anything to be concerned about, as it will be removed by the Ion OneTouch ES.

What should I do if the centrifuge is still running even though the time remaining is 0:00 at the final spin of the Ion OneTouch 2?

The time remaining readout is not entirely accurate. The centrifuge may keep rotating for about 15 minutes after the time has become 0:00. If it rotates for more than 15 minutes, please contact Technical Support.

Ion Chef System

I am getting consistently low loading from the same chip position with the Ion Torrent™ Ion Chef™ System. What should I do?

The Ion Chef robot arm may need recalibration. Please call 1-87-SEQUENCE to open a service call.

The chip/chip adapter does not fit into the chip bucket. What should I do?

Please ensure that you are using the universal chip bucket. Universal chip buckets have an etching on the corners indicating the chip barcode position. If you do not have the universal chip bucket, please call technical support at 1-87-SEQUENCE.

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