Invitrogen

CD4 Monoclonal Antibody (RM4-5), FITC

FIGURE: 1 / 6

CD4 Antibody (MCD0401) in Flow

C57BL/6 splenocytes were left unstimulated or stimulated for 5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. Cells were then surface-stained with anti-mouse CD4 FITC (Product # MCD0401) and subsequently fixed and permeabilized using the FIX & PERM® Cell Permeabilization Kit (Product # GAS-003) and stained intracellularly with anti-mouse interferon gamma (IFN-gamma) PE-Cy®7 conjugate (Product # A18713) and anti-mouse tumor necrosis factor alpha (TNF-a) Pacific Blue™ conjugate (Product # RM90128). S... View More

CD4 Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

MCD0401

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Immunogen

Mouse CD4

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_10375031

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Optimized protocols for isolation, fixation, and flow cytometric characterization of leukocytes in ischemic hearts.

American journal of physiology. Heart and circulatory physiology

Covarrubias R,Ismahil MA,Rokosh G,Hamid T,Accornero F,Singh H,Gumina RJ,Prabhu SD,Bansal SS

MCD0401 was used in Flow cytometry/Cell sorting to develop an enhanced method of isolating, fixing and characterising cardiac CD45+ leukocytes in ischemic hearts.

Sun Sep 01 00:00:00 EDT 2019

Toll-like receptor 3 (TLR3) promotes the resolution of Chlamydia muridarum genital tract infection in congenic C57BL/6N mice.

PloS one

Carrasco SE,Hu S,Imai DM,Kumar R,Sandusky GE,Yang XF,Derbigny WA

MCD0401 was used in Flow cytometry/Cell sorting to examine the role of Toll-like receptor 3 in the clearance of Chlamydia during early and mid-stages of genital tract infection.

Mon Jul 16 00:00:00 EDT 2018

Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren's syndrome mice.

Scientific reports

Wanchoo A,Voigt A,Sukumaran S,Stewart CM,Bhattacharya I,Nguyen CQ

Published figure using CD4 monoclonal antibody (Product # MCD0401) in Immunofluorescence

Mon Oct 02 00:00:00 EDT 2017

Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing.

Journal of immunology (Baltimore, Md. : 1950)

Tsuboi K,Hirakawa J,Seki E,Imai Y,Yamaguchi Y,Fukuda M,Kawashima H

MCD0401 was used in Flow cytometry/Cell sorting to suggest that chemokine presentation by heparan sulfate on the surface of high endothelial venules facilitates lymphocyte homing.

Mon Jul 01 00:00:00 EDT 2013

The ΔfbpA attenuated candidate vaccine from Mycobacterium tuberculosis, H37Rv primes for a stronger T-bet dependent Th1 immunity in mice.

Tuberculosis (Edinburgh, Scotland)

Roche CM,Smith A,Lindsey DR,Meher A,Schluns K,Arora A,Armitige LY,Jagannath C

Published figure using CD4 monoclonal antibody (Product # MCD0401) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

A defect in the synthesis of Interferon-γ by the T cells of Complement-C5 deficient mice leads to enhanced susceptibility for tuberculosis.

Tuberculosis (Edinburgh, Scotland)

Mashruwala MA,Smith AK,Lindsey DR,Moczygemba M,Wetsel RA,Klein JR,Actor JK,Jagannath C

MCD0401 was used in Flow cytometry/Cell sorting to demonstrate here that, the C5(-/-) derived CD3(+) T cells, have an additional defect in the synthesis of IFNγ.

Thu Dec 01 00:00:00 EST 2011

Absence of IL-23p19 in donor allogeneic cells reduces mortality from acute GVHD.

Bone marrow transplantation

Thompson JS,Chu Y,Glass JF,Brown SA

MCD0401 was used in flow cytometry to test if deficiency of p19 in the allogeneic donor transplant reduces the inflammation caused by acute GVHD

Thu Apr 01 00:00:00 EDT 2010

The manganese superoxide dismutase mimetic, M40403, protects adult mice from lethal total body irradiation.

Free radical research

Thompson JS,Chu Y,Glass J,Tapp AA,Brown SA

MCD0401 was used in flow cytometry to report that a non-peptidyl mimetic of MnSOD reduces total body irradiation-induced tissue damage

Sat May 01 00:00:00 EDT 2010

Complement C5a anaphylatoxin is an innate determinant of dendritic cell-induced Th1 immunity to Mycobacterium bovis BCG infection in mice.

Journal of leukocyte biology

Moulton RA,Mashruwala MA,Smith AK,Lindsey DR,Wetsel RA,Haviland DL,Hunter RL,Jagannath C

MCD0401 was used in flow cytometry to assess the effects of C5a on IL-12p70 secretion by murine dendritic cells and Th1 immunity

Mon Oct 01 00:00:00 EDT 2007

Role of T cells in innate and adaptive immunity against murine Burkholderia pseudomallei infection.

The Journal of infectious diseases

Haque A,Easton A,Smith D,O'Garra A,Van Rooijen N,Lertmemongkolchai G,Titball RW,Bancroft GJ

MCD0401 was used in flow cytometry to study the in vivo role of cytokine- and antigen-mediated T cell activation in the resistance to Burkholderia pseudomallei

Wed Feb 01 00:00:00 EST 2006

Overexpression of the Runx3 transcription factor increases the proportion of mature thymocytes of the CD8 single-positive lineage.

Journal of immunology (Baltimore, Md. : 1950)

Kohu K,Sato T,Ohno S,Hayashi K,Uchino R,Abe N,Nakazato M,Yoshida N,Kikuchi T,Iwakura Y,Inoue Y,Watanabe T,Habu S,Satake M

MCD0401 was used in flow cytometry to report that Runx3 drives thymocytes to select the CD4-8+ lineage

Tue Mar 01 00:00:00 EST 2005

Oral administration of the bacterial superantigen staphylococcal enterotoxin B induces activation and cytokine production by T cells in murine gut-associated lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Spiekermann GM,Nagler-Anderson C

MCD0401 was used in flow cytometry to compare gut and peripheral T cell responses after staphylococcal enterotoxin treatment.

Tue Dec 01 00:00:00 EST 1998

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD4 Monoclonal Antibody (RM4-5), FITC

CD4 Antibody (MCD0401) in Flow

Product Details

MCD0401

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Performance Guarantee

We're here to help

Your items have has been added!