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Invitrogen
P-Selectin Recombinant Rabbit Monoclonal Antibody (3H20L10)
FIGURE: 1 / 5
P-Selectin Antibody (701257) in ICC/IF
Immunofluorescent analysis of P-selectin in U2OS cells using a P-selectin recombinant rabbit monoclonal antibody (Product # 701257) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing membrane localizatio... View More
Product Details
701257
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
3H20L10
Immunogen
Peptide corresponding to amino acids 113-126 of human P-selectin
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532443
Product Specific Information
This antibody is predicted to react with mouse, rat and non-human primate based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)
Gene Aliases: CD62; CD62P; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP
UniProt ID: (Human) P16109
Entrez Gene ID: (Human) 6403
platelet degranulation
positive regulation of leukocyte migration
regulation of cellular extravasation
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
positive regulation of platelet activation
positive regulation of phosphatidylinositol 3-kinase signaling
response to organic cyclic compound
response to lipopolysaccharide
regulation of integrin activation
calcium-mediated signaling using intracellular calcium source
positive regulation of cell adhesion
defense response to Gram-negative bacterium
leukocyte migration
leukocyte tethering or rolling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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