Invitrogen

Fyn Monoclonal Antibody (FYN-01)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

4 References

View all (36) Fyn antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Fyn Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

Fyn Antibody (MA1-19331) in ICC/IF

Immunocytochemistry analysis of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green) Monoclonal antibody (Product # MA1-19331) using a dilution of 5 µg/mL. Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Fyn Antibody in Immunoprecipitation (IP)

Product Image Gallery

Fyn Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. A higher level expression of Fyn was observed in Ramos, MOLT-4 and Raji compared to HEK293, MCF-7 and PC-12 using Fyn Monoclonal Antibody (FYN-01) (Product # MA1-19331) in western blot. {RE}

Product Details

MA1-19331

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

FYN-01

Immunogen

Recombinant fragment of human Fyn (aa 7-176)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074491

Product Specific Information

This antibody recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).

Immunoprecipitation: Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitants Fyn from cell lysates.

Target Information

FYN is a SRC-related kinase expressed in brain, skeletal muscle and adipose tissues. Fyn functions in a large number of pathways and has been shown to play a role in memory. FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Science advances

Roberts JA,Varma VR,An Y,Varma S,Candia J,Fantoni G,Tiwari V,Anerillas C,Williamson A,Saito A,Loeffler T,Schilcher I,Moaddel R,Khadeer M,Lovett J,Tanaka T,Pletnikova O,Troncoso JC,Bennett DA,Albert MS,Yu K,Niu M,Haroutunian V,Zhang B,Peng J,Croteau DL,Resnick SM,Gorospe M,Bohr VA,Ferrucci L,Thambisetty M

Published figure using Fyn monoclonal antibody (Product # MA1-19331) in Western Blot

Fri Nov 12 00:00:00 EST 2021

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Science advances

Published figure using Fyn monoclonal antibody (Product # MA1-19331) in Western Blot

Fri Nov 12 00:00:00 EST 2021

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Science advances

Published figure using Fyn monoclonal antibody (Product # MA1-19331) in Western Blot

Fri Nov 12 00:00:00 EST 2021

Human CEACAM1-LF regulates lipid storage in HepG2 cells via fatty acid transporter CD36.

The Journal of biological chemistry

Chean J,Chen CJ,Gugiu G,Wong P,Cha S,Li H,Nguyen T,Bhatticharya S,Shively JE

MA1-19331 was used in Western Blot to find that the fatty acid transporter CD36 was upregulated in the S508A mutant, coexpressed in BCs with CEACAM1, co-IPed with CEACAM1 and Src, and when downregulated via RNAi, an increase in lipid droplet content was observed.

Mon Nov 01 00:00:00 EDT 2021

Fyn-tau Ablation Modifies PTZ-Induced Seizures and Post-seizure Hallmarks of Early Epileptogenesis.

Frontiers in cellular neuroscience

Putra M,Puttachary S,Liu G,Lee G,Thippeswamy T

MA1-19331 was used in Western Blotting to demonstrate the role of Fyn and tau in seizures and their impact on the mediators of early epileptogenesis in PTZ model.

Tue Feb 02 00:00:00 EST 2021

Oxidation of KCNB1 potassium channels triggers apoptotic integrin signaling in the brain.

Cell death & disease

Yu W,Gowda M,Sharad Y,Singh SA,Sesti F

Published figure using Fyn monoclonal antibody (Product # MA1-19331) in Western Blot

Thu Apr 06 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: C syn protooncogene; c-syn protooncogene; EC 2.7.10.2; FYN oncogene related to SRC, FGR, YES; kinase Fyn; MGC45350; OKT3-induced calcium influx regulator; OTTHUMP00000017916; OTTHUMP00000017917; P59 p59-Fyn; p59-Fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase Fyn; Protooncogene Syn; RP1-66H14.1; SLK; Src Kinase p59; Src like kinase; Src-like kinase; src/yes-related novel; tyrosine kinase p59fyn(T); Tyrosine-protein kinase Fyn

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Gene Aliases: AI448320; AW552119; FYN; p59-FYN; SLK; SYN

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UniProt ID: (Human) P06241, (Mouse) P39688

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Entrez Gene ID: (Human) 2534, (Mouse) 14360

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Function(s)

G-protein coupled receptor binding glycoprotein binding protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding tubulin binding T cell receptor binding CD4 receptor binding CD8 receptor binding phosphatidylinositol 3-kinase binding ion channel binding metal ion binding ephrin receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity peptide hormone receptor binding growth factor receptor binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding kinase modulator

Process(es)

MAPK cascade neuron migration stimulatory C-type lectin receptor signaling pathway adaptive immune response protein phosphorylation calcium ion transport transmembrane receptor protein tyrosine kinase signaling pathway axon guidance central nervous system development blood coagulation learning feeding behavior regulation of cell shape negative regulation of gene expression positive regulation of neuron projection development regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling viral process cell migration peptidyl-tyrosine phosphorylation cell differentiation platelet activation forebrain development T cell costimulation negative regulation of protein ubiquitination intracellular signal transduction cellular response to platelet-derived growth factor stimulus peptidyl-tyrosine autophosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis T cell activation regulation of cell proliferation negative regulation of protein catabolic process response to drug positive regulation of tyrosine phosphorylation of Stat5 protein regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neuron apoptotic process positive regulation of GTPase activity innate immune response response to ethanol phosphatidylinositol phosphorylation vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway phosphatidylinositol-mediated signaling dendrite morphogenesis regulation of defense response to virus by virus activated T cell proliferation T cell receptor signaling pathway leukocyte migration detection of mechanical stimulus involved in sensory perception of pain cellular response to peptide hormone stimulus cellular response to transforming growth factor beta stimulus positive regulation of protein localization to nucleus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand immune system process cell surface receptor signaling pathway multicellular organism development phosphorylation myelination protein autophosphorylation

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Fyn Monoclonal Antibody (FYN-01)

Fyn Antibody (MA1-19331) in ICC/IF

Product Details

MA1-19331

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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