Invitrogen

HLA-ABC Monoclonal Antibody (W6/32), Functional Grade

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure

12 References

View all (73) HLA-ABC antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HLA-ABC Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

HLA-ABC Antibody (MA1-19027) in Flow

Flow cytometry analysis of HLA-ABC using a monoclonal antibody (Product # MA1-19027).

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Product Details

MA1-19027

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

- -

Inhibition Assays (IA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Cat, Human, Non-human primate

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

W6/32

Immunogen

Membrane of human tonsil cells.

View immunogen

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076699

Product Specific Information

This antibody will not cross-react with rabbit.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Purity is >95% by SDS-PAGE.

Western blot should be performed under non-reducing conditions. It has also been successfully used in Mass Cytometry (CyTof).

Target Information

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Patient-derived monoclonal antibody neutralizes SARS-CoV-2 Omicron variants and confers full protection in monkeys.

Nature microbiology

Fenwick C,Turelli P,Ni D,Perez L,Lau K,Herate C,Marlin R,Lana E,Pellaton C,Raclot C,Esteves-Leuenberger L,Campos J,Farina A,Fiscalini F,Dereuddre-Bosquet N,Relouzat F,Abdelnabi R,Foo CS,Neyts J,Leyssen P,Lévy Y,Pojer F,Stahlberg H,LeGrand R,Trono D,Pantaleo G

MA1-19027 was used in Flow cytometry/Cell sorting to provide additional therapies against Omicron, we isolated a mAb named P2G3 from a previously infected vaccinated donor and showed that it has picomolar-range neutralizing activity against Omicron BA.1, BA.1.1, BA.2 and all other variants tested.

Thu Sep 01 00:00:00 EDT 2022

Tumor organoid-T-cell coculture systems.

Nature protocols

Cattaneo CM,Dijkstra KK,Fanchi LF,Kelderman S,Kaing S,van Rooij N,van den Brink S,Schumacher TN,Voest EE

MA1-19027 was used in Inhibition Assays to enable the establishment of ex vivo test systems for T-cell-based immunotherapy at the level of the individual patient.

Wed Jan 01 00:00:00 EST 2020

A humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells.

Nature medicine

Reinisch A,Thomas D,Corces MR,Zhang X,Gratzinger D,Hong WJ,Schallmoser K,Strunk D,Majeti R

MA119027 was used in flow cytometry to develop and characterize a humanized ossicle xenotransplantation approach

Fri Jul 01 00:00:00 EDT 2016

Hepatitis C virus infection in inclusion body myositis: A case-control study.

Neurology

Uruha A,Noguchi S,Hayashi YK,Tsuburaya RS,Yonekawa T,Nonaka I,Nishino I

MA1-19027 was used in immunohistochemistry - paraffin section to determine if there is an association between inclusion body myositis and hepatitis C virus infection

Tue Jan 19 00:00:00 EST 2016

MICA Expression Is Regulated by Cell Adhesion and Contact in a FAK/Src-Dependent Manner.

Frontiers in immunology

Moncayo G,Lin D,McCarthy MT,Watson AA,O'Callaghan CA

MA1-19027 was used in Flow cytometry/Cell sorting to study the way in which tumour cells avoid immune recognition during metastasis, possibly via the reduction of MICA expression following loss of attachment.

Thu Mar 04 00:00:00 EST 2021

Interferon-γ induces immunoproteasomes and the presentation of MHC I-associated peptides on human salivary gland cells.

PloS one

Arellano-Garcia ME,Misuno K,Tran SD,Hu S

MA1-19027 was used in immunoprecipitation and western blot to study lactacystin and IFN-gamma in human salivary glands

Tue Apr 21 00:00:00 EDT 2015

Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.

mBio

Olofsson A,Nygård Skalman L,Obi I,Lundmark R,Arnqvist A

MA1-19027 was used in immunocytochemistry to study the role of clathrin-dependent and clathrin-independent routes in the uptake of Helicobacter pylori vesicles by gastric epithelial cells

Tue May 20 00:00:00 EDT 2014

Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.

Anticancer research

Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK

MA1-19027 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells

Fri Nov 01 00:00:00 EDT 2013

The human herpesvirus-7 (HHV-7) U21 immunoevasin subverts NK-mediated cytoxicity through modulation of MICA and MICB.

PLoS pathogens

Schneider CL,Hudson AW

MA1-19027 was used in western blot to report that the human herpesvirus-7 U21 gene product interferes with natural killer cell recognition

Tue Nov 01 00:00:00 EDT 2011

Induction of metastatic cancer stem cells from the NK/LAK-resistant floating, but not adherent, subset of the UP-LN1 carcinoma cell line by IFN-γ.

Laboratory investigation; a journal of technical methods and pathology

Chen HC,Chou AS,Liu YC,Hsieh CH,Kang CC,Pang ST,Yeh CT,Liu HP,Liao SK

MA1-19027 was used in flow cytometry to study the induction of metastatic cancer stem cells from UP-LN1 cell line

Sat Oct 01 00:00:00 EDT 2011

Alterations in the Arf6-regulated plasma membrane endosomal recycling pathway in cells overexpressing the tetraspan protein Gas3/PMP22.

Journal of cell science

Chies R,Nobbio L,Edomi P,Schenone A,Schneider C,Brancolini C

MA1-19027 was used in immunocytochemistry to study alterations in Arf6-regulated plasma membrane endosomal recycling in cells overexpressing Gas3/PMP22

Sat Mar 15 00:00:00 EST 2003

Establishment of a quantitative ELISA capable of determining peptide - MHC class I interaction.

Tissue antigens

Sylvester-Hvid C,Kristensen N,Blicher T,Ferré H,Lauemøller SL,Wolf XA,Lamberth K,Nissen MH,Pedersen LØ,Buus S

MA1-19027 was used in ELISA to develop a quantitative ELISA assay for studying peptide-MHC class I interactions

Mon Apr 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: A29 protein; alpha chain of MHC class II; antigen presenting molecule; class I MHC, HLA-B antigen; epididymis secretory protein Li 83; H-2K; H-2K(d); H2-K; histocompatibility antigen; HLA -B; HLA A; HLA B-40011; HLA B44; HLA C; HLA class I antigen; HLA class I antigen HLA-B; HLA class I histocompatibility antigen C alpha chain; HLA class I histocompatibility antigen Cw-1 alpha chain; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-30 alpha chain; HLA class I histocompatibility antigen, A-33 alpha chain; HLA class I histocompatibility antigen, A-74 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, B-47 alpha chain; HLA class I histocompatibility antigen, B-50 alpha chain; HLA class I histocompatibility antigen, B-82 alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA class I histocompatibility antigen, Cw-15 alpha chain; HLA class I histocompatibility antigen, Cw-18 alpha chain; HLA locus; HLA molecule; HLA-A; HLA-A locus alpha 2 domain; HLA-A null; HLA-Aw33.1; HLA-B; HLA-B 1502; HLA-B alpha chain; HLA-B alpha chain (B*2706); HLA-B alpha chain (B*5002); HLA-B alpha-chain; HLA-B35; HLA-B35 antigen; HLA-Bw50 antigen; HLA-C; HLA-C alpha chain; HLA-C alpha chain (HLA-Cw*15v); HLA-C alpha chain for Cw*18GB; HLA-C alpha-chain; HLA-C antigen; HLA-Cw; HLA-Cw*04GB; HLA-Cw*1702; HLA-Cw*18GB; HLA-Cw-1503; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; hypothetical protein; lecocyte antigen B; leucocyte antigen A; Leukocyte Antigen; leukocyte antigen C; leukocyte antigen class I-A; leukocyte antigen class I-B; Leukoocyte Antigen; lymphocyte antigen; major histocompatability complex B antigen; major histocompatibility antigen HLA-C; MCH class I antigen; MGC184092; MGC7052; MHC 1; MHC antigen; MHC clas I antigen; MHC Class 1; MHC class 1 antigen; MHC class I; MHC class I alpha chain; MHC class I anitgen; MHC class I anti; MHC Class I antigen; MHC class I antigen A*30; MHC class I antigen A*33; MHC class I antigen A*74; MHC class I antigen B*47; MHC class I antigen B*50; MHC class I antigen B*82; MHC class I antigen Cw*15; MHC class I antigen Cw*18; MHC class I antigen GN00104; MHC class I antigen heavy chain; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-A33; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen null protein; MHC class I antigen SHCHA; MHC class I antigene; MHC class I histocompatibility antigen; MHC class I human leukocyte antigen; MHC class I molecule; MHC class I protein; MHC class II antigen; MHC class l antigen; MHC classI antigen; MHC HLA-ABC; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B27-HS; MHC HLA-B27d; MHC HLA-B39N; MHC I HLA; MHC1; surface antigen; truncated class I antigen; truncated MHC class I antigen

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Gene Aliases: AS; Aw-33; Aw-74; B-4901; B-5001; Bw-47; Bw-50; D6S204; HEL-S-83; HLA-A; HLA-A11; HLA-A33; HLA-B; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HLA-C; HLA-Cw; HLA-DQB1; HLA-DRB1; HLA-JY3; HLAA; HLAB; HLAC; HLC-C; MHC; PSORS1; SPDA1

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UniProt ID: (Human) Q9TQM6, (Human) Q31603, (Human) Q29990

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Entrez Gene ID: (Human) 3105, (Human) 3106, (Human) 3107

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Function(s)

protein binding beta-2-microglobulin binding peptide antigen binding poly(A) RNA binding antigen binding major histocompatibility complex protein

Process(es)

antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent viral process antigen processing and presentation regulation of immune response interferon-gamma-mediated signaling pathway type I interferon signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HLA-ABC Monoclonal Antibody (W6/32), Functional Grade

HLA-ABC Antibody (MA1-19027) in Flow

Product Details

MA1-19027

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Functional Assay (Functional)

Inhibition Assays (IA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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