Invitrogen

ICAM-1 Monoclonal Antibody (1A29)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

22 Published Figures

30 References

View all (173) ICAM-1 (CD54) antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

ICAM-1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 32

ICAM-1 Antibody (MA5407) in ICC/IF

Immunofluorescence analysis of ICAM-1/CD54 was done on 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ICAM-1/CD54 (1A29) Mouse Monoclonal Antibody (Product # MA5407) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More

ICAM-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ICAM-1 Antibody in Flow Cytometry (Flow)

ICAM-1 Antibody in Western Blot, Immunocytochemistry, Neutralization (WB, ICC/IF, Neu)

ICAM-1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

ICAM-1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ICAM-1 Antibody in Immunohistochemistry (IHC)

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Product Details

MA5407

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 7 publications 7 publications

Flow Cytometry (Flow)

1:20

Neutralization (Neu)

Assay-dependent

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A29

Immunogen

Rat lymph node stroma

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223596

Product Specific Information

MA5407 targets CD54 in FACS, IHC, and Neu applications and shows reactivity with Rat, Human and Mouse samples.

The MA5407 immunogen is rat lymph node stroma.

MA5407 detects CD54 which has a predicted molecular weight of approximately 56 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modelling metastasis in zebrafish unveils regulatory interactions of cancer-associated fibroblasts with circulating tumour cells.

Frontiers in cell and developmental biology

Hurtado P,Martínez-Pena I,Yepes-Rodríguez S,Bascoy-Otero M,Abuín C,Fernández-Santiago C,Sánchez L,López-López R,Piñeiro R

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Mar 24 00:00:00 EDT 2023

Modelling metastasis in zebrafish unveils regulatory interactions of cancer-associated fibroblasts with circulating tumour cells.

Frontiers in cell and developmental biology

Hurtado P,Martínez-Pena I,Yepes-Rodríguez S,Bascoy-Otero M,Abuín C,Fernández-Santiago C,Sánchez L,López-López R,Piñeiro R

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Mar 24 00:00:00 EDT 2023

Modelling metastasis in zebrafish unveils regulatory interactions of cancer-associated fibroblasts with circulating tumour cells.

Frontiers in cell and developmental biology

Hurtado P,Martínez-Pena I,Yepes-Rodríguez S,Bascoy-Otero M,Abuín C,Fernández-Santiago C,Sánchez L,López-López R,Piñeiro R

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Mar 24 00:00:00 EDT 2023

Uptake and anti-inflammatory effects of liposomal astaxanthin on endothelial cells tracked by Raman and fluorescence imaging.

Mikrochimica acta

Radwan B,Prabhakaran A,Rocchetti S,Matuszyk E,Keyes TE,Baranska M

MA5407 was used in Immunocytochemistry-immunofluorescence to highlight the benefits of AXT liposomal encapsulation on EC and the applicability of Raman imaging to investigate such effects.

Thu Jul 27 00:00:00 EDT 2023

Neuroprotective effects of Alda-1 mitigate spinal cord injury in mice: involvement of Alda-1-induced ALDH2 activation-mediated suppression of reactive aldehyde mechanisms.

Neural regeneration research

Khan M,Qiao F,Kumar P,Touhidul Islam SM,Singh AK,Won J,Singh I

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Sat Jan 01 00:00:00 EST 2022

Murine Beta-Amyloid (1-42) Oligomers Disrupt Endothelial Barrier Integrity and VEGFR Signaling via Activating Astrocytes to Release Deleterious Soluble Factors.

International journal of molecular sciences

Yue Q,Zhou X,Zhang Z,Hoi MPM

MA5407 was used in Immunocytochemistry, Western Blot to show that murine sAβO42 was moderately toxic to an endothelial and astrocyte co-culture.

Mon Feb 07 00:00:00 EST 2022

Murine Beta-Amyloid (1-42) Oligomers Disrupt Endothelial Barrier Integrity and VEGFR Signaling via Activating Astrocytes to Release Deleterious Soluble Factors.

International journal of molecular sciences

Yue Q,Zhou X,Zhang Z,Hoi MPM

MA5407 was used in Immunocytochemistry, Western Blot to show that murine sAβO42 was moderately toxic to an endothelial and astrocyte co-culture.

Mon Feb 07 00:00:00 EST 2022

Murine Beta-Amyloid (1-42) Oligomers Disrupt Endothelial Barrier Integrity and VEGFR Signaling via Activating Astrocytes to Release Deleterious Soluble Factors.

International journal of molecular sciences

Yue Q,Zhou X,Zhang Z,Hoi MPM

MA5407 was used in Immunocytochemistry, Western Blot to show that murine sAβO42 was moderately toxic to an endothelial and astrocyte co-culture.

Mon Feb 07 00:00:00 EST 2022

Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells.

Fluids and barriers of the CNS

Walter FR,Harazin A,Tóth AE,Veszelka S,Santa-Maria AR,Barna L,Kincses A,Biczó G,Balla Z,Kui B,Maléth J,Cervenak L,Tubak V,Kittel Á,Rakonczay Z,Deli MA

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Immunocytochemistry

Thu Feb 17 00:00:00 EST 2022

Blood-brain barrier dysfunction in L-ornithine induced acute pancreatitis in rats and the direct effect of L-ornithine on cultured brain endothelial cells.

Fluids and barriers of the CNS

Walter FR,Harazin A,Tóth AE,Veszelka S,Santa-Maria AR,Barna L,Kincses A,Biczó G,Balla Z,Kui B,Maléth J,Cervenak L,Tubak V,Kittel Á,Rakonczay Z,Deli MA

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Immunocytochemistry

Thu Feb 17 00:00:00 EST 2022

Extracellular Inflammasome Particles Are Released After Marathon Running and Induce Proinflammatory Effects in Endothelial Cells.

Frontiers in physiology

Kogel A,Fikenzer S,Uhlmann L,Opitz L,Kneuer JM,Haeusler KG,Endres M,Kratzsch J,Schwarz V,Werner C,Kalwa H,Gaul S,Laufs U

MA5407 was used in Immunocytochemistry-immunoflourescence to identify extracellular inflammasome particles as novel systemic mediators of cell-cell communication that are transiently increased after acute extensive exercise with a high mechanical muscular load.

Sat Jul 16 00:00:00 EDT 2022

Extracellular Inflammasome Particles Are Released After Marathon Running and Induce Proinflammatory Effects in Endothelial Cells.

Frontiers in physiology

Kogel A,Fikenzer S,Uhlmann L,Opitz L,Kneuer JM,Haeusler KG,Endres M,Kratzsch J,Schwarz V,Werner C,Kalwa H,Gaul S,Laufs U

Sat Jul 16 00:00:00 EDT 2022

Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.

Fluids and barriers of the CNS

Sifat AE,Archie SR,Nozohouri S,Villalba H,Zhang Y,Sharma S,Ghanwatkar Y,Vaidya B,Mara D,Cucullo L,Abbruscato TJ

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Sep 09 00:00:00 EDT 2022

Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.

Fluids and barriers of the CNS

Sifat AE,Archie SR,Nozohouri S,Villalba H,Zhang Y,Sharma S,Ghanwatkar Y,Vaidya B,Mara D,Cucullo L,Abbruscato TJ

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Sep 09 00:00:00 EDT 2022

Human neural cell type-specific extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain.

Journal of extracellular vesicles

You Y,Muraoka S,Jedrychowski MP,Hu J,McQuade AK,Young-Pearse T,Aslebagh R,Shaffer SA,Gygi SP,Blurton-Jones M,Poon WW,Ikezu T

MA5407 was used in Western Blotting to identify cell type-specific extracellular vesicle (EV) protein markers in human-induced pluripotent stem cell-derived neural cell types, including excitatory neurons (ATP1A3, NCAM1), astrocytes (LRP1, ITGA6), microglia-like cells (ITGAM, LCP1), and oligodendrocyte-like cells (LAMP2, FTH1), along with 16 pan-EV marker candidates.

Sat Jan 01 00:00:00 EST 2022

AIM2 deletion enhances blood-brain barrier integrity in experimental ischemic stroke.

CNS neuroscience & therapeutics

Xu SY,Bian HJ,Shu S,Xia SN,Gu Y,Zhang MJ,Xu Y,Cao X

MA5407 was used in Western Blot to explore the effects of AIM2 on the BBB integrity in mice with middle cerebral artery occlusion induced injury and in oxygen-glucose deprivation/reoxygenation induced human brain microvascular endothelial cells.

Fri Oct 01 00:00:00 EDT 2021

AIM2 deletion enhances blood-brain barrier integrity in experimental ischemic stroke.

CNS neuroscience & therapeutics

Xu SY,Bian HJ,Shu S,Xia SN,Gu Y,Zhang MJ,Xu Y,Cao X

Fri Oct 01 00:00:00 EDT 2021

Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury.

Theranostics

Cao JY,Wang B,Tang TT,Wen Y,Li ZL,Feng ST,Wu M,Liu D,Yin D,Ma KL,Tang RN,Wu QL,Lan HY,Lv LL,Liu BC

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Wed Jul 28 00:00:00 EDT 2021

TRPV4 channel activation induces the transition of venous and arterial endothelial cells toward a pro-inflammatory phenotype.

Physiological reports

Beddek K,Raffin F,Borgel D,Saller F,Riccobono D,Bobe R,Boittin FX

MA5407 was used in Immunocytochemistry-immunoflourescence to investigate possible links between TRPV4 activation and development of pro-inflammatory properties of endothelial cells.

Mon Feb 01 00:00:00 EST 2021

TRPV4 channel activation induces the transition of venous and arterial endothelial cells toward a pro-inflammatory phenotype.

Physiological reports

Beddek K,Raffin F,Borgel D,Saller F,Riccobono D,Bobe R,Boittin FX

MA5407 was used in Immunocytochemistry-immunoflourescence to investigate possible links between TRPV4 activation and development of pro-inflammatory properties of endothelial cells.

Mon Feb 01 00:00:00 EST 2021

Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.

eNeuro

Bhowmick S,Malat A,Caruso D,Ponery N,D'Mello V,Finn C,Abdul-Muneer PM

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Aug 13 00:00:00 EDT 2021

Intercellular Adhesion Molecule-1-Induced Posttraumatic Brain Injury Neuropathology in the Prefrontal Cortex and Hippocampus Leads to Sensorimotor Function Deficits and Psychological Stress.

eNeuro

Bhowmick S,Malat A,Caruso D,Ponery N,D'Mello V,Finn C,Abdul-Muneer PM

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Aug 13 00:00:00 EDT 2021

GSNOR and ALDH2 alleviate traumatic spinal cord injury.

Brain research

Khan M,Qiao F,Islam SMT,Dhammu TS,Kumar P,Won J,Singh AK,Singh I

MA5407 was used in Western Blotting to test the hypotheses that S-nitrosoglutathione reductase (GSNOR) 's specific inhibitor N6022 mitigates pain and improves functional recovery in a mouse model of spinal cord injury (SCI).

Sat May 01 00:00:00 EDT 2021

The cancer glycocalyx mediates intravascular adhesion and extravasation during metastatic dissemination.

Communications biology

Offeddu GS,Hajal C,Foley CR,Wan Z,Ibrahim L,Coughlin MF,Kamm RD

MA5407 was used in Simple Western - Size to further understand how tumour cells escape the vascular system to initiate metastatic sites.

Fri Feb 26 00:00:00 EST 2021

Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo.

Journal of oral microbiology

Reyes L,Getachew H,Dunn WA,Progulske-Fox A

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Neutralization

Fri May 07 00:00:00 EDT 2021

Porphyromonas gingivalis W83 traffics via ICAM1 in microvascular endothelial cells and alters capillary organization in vivo.

Journal of oral microbiology

Reyes L,Getachew H,Dunn WA,Progulske-Fox A

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Neutralization

Fri May 07 00:00:00 EDT 2021

Macrophage migration inhibitory factor inhibition as a novel therapeutic approach against triple-negative breast cancer.

Cell death & disease

Charan M,Das S,Mishra S,Chatterjee N,Varikuti S,Kaul K,Misri S,Ahirwar DK,Satoskar AR,Ganju RK

MA5407 was used in Immunohistochemistry to study the role of Macrophage migration inhibitory factor (MIF) in Triple-negative breast cancer (TNBC) progression and metastasis.

Thu Sep 17 00:00:00 EDT 2020

MicroRNA-200a/200b Modulate High Glucose-Induced Endothelial Inflammation by Targeting O-linked N-Acetylglucosamine Transferase Expression.

Frontiers in physiology

Lo WY,Yang WK,Peng CT,Pai WY,Wang HJ

MA5407 was used in Immunohistochemistry to examine whether miR-200a and miR-200b regulate high-glucose-induced O-GlcNAc transferase expression in human aortic endothelial cells and whether they control high-glucose-induced endothelial inflammation.

Sat Apr 09 00:00:00 EDT 2022

Notoginsenoside R1 Protects HUVEC Against Oxidized Low Density Lipoprotein (Ox-LDL)-Induced Atherogenic Response via Down-Regulating miR-132.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Fu C,Yin D,Nie H,Sun D

MA5407 was used in Western Blotting to show NGR1 prevents ox-LDL-induced apoptosis, migration and adhesion-related molecule release in HUVECs possibly via down-regulating miR-132, and subsequent up-regulating MGP.

Mon Jan 21 00:00:00 EST 2019

A novel pathway of LPS uptake through syndecan-1 leading to pyroptotic cell death.

eLife

Yokoyama S,Cai Y,Murata M,Tomita T,Yoneda M,Xu L,Pilon AL,Cachau RE,Kimura S

MA5407 was used in Immunocytochemistry-immunoflourescence to identify a novel mechanism whereby lipopolysaccharide enters innate immune cells leading to pyroptosis, and clarify the effect of lipopolysaccharide on cancer cells.

Fri Dec 07 00:00:00 EST 2018

Liver graft preservation using perfluorocarbon improves the outcomes of simulated donation after cardiac death liver transplantation in rats.

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

Okumura S,Uemura T,Zhao X,Masano Y,Tsuruyama T,Fujimoto Y,Iida T,Yagi S,Bezinover D,Spiess B,Kaido T,Uemoto S

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Immunohistochemistry (Frozen)

Fri Sep 01 00:00:00 EDT 2017

K_Ca3.1 channel inhibition leads to an ICAM-1 dependent increase of cell-cell adhesion between A549 lung cancer and HMEC-1 endothelial cells.

Oncotarget

Bulk E,Kramko N,Liashkovich I,Glaser F,Schillers H,Schnittler HJ,Oberleithner H,Schwab A

Published figure using ICAM-1 monoclonal antibody (Product # MA5407) in Western Blot

Fri Dec 22 00:00:00 EST 2017

Thoracic epidural bupivacaine attenuates inflammatory response, intestinal lipid peroxidation, oxidative injury, and mucosal apoptosis induced by mesenteric ischemia/reperfusion.

Anesthesia and analgesia

Bedirli N,Akyürek N,Kurtipek O,Kavutcu M,Kartal S,Bayraktar AC

MA5407 was used in immunohistochemistry to investigate the effectivenes of thoracic epidural anesthesia in mesenteric ischemia/reperfusion

Tue Nov 01 00:00:00 EDT 2011

NAD(P)H oxidase mediates angiotensin II-induced vascular macrophage infiltration and medial hypertrophy.

Arteriosclerosis, thrombosis, and vascular biology

Liu J,Yang F,Yang XP,Jankowski M,Pagano PJ

MA5407 was used in immunohistochemistry to study the role of NAD(P)H oxidase in angiotensin II-induced vascular macrophage infiltration and medial hypertrophy

Thu May 01 00:00:00 EDT 2003

Adhesion molecule expression in experimental myositis.

Muscle & nerve

Ito T,Kumamoto T,Horinouchi H,Yukishige K,Sugihara R,Fujimoto S,Tsuda T

MA5407 was used in immunohistochemistry and western blot to elucidate the role of adhesion molecules in the pathogenesis of experimental allergic myositis

Fri Mar 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: BB2; CD54; ICAM; Icam-1; ICAM1; Ly-47; MALA-2; P3.58

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UniProt ID: (Human) P05362, (Mouse) P13597, (Rat) Q00238

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Entrez Gene ID: (Human) 3383, (Mouse) 15894, (Rat) 25464

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Function(s)

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding protein complex binding

Process(es)

Performance Guarantee

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ICAM-1 Monoclonal Antibody (1A29)

ICAM-1 Antibody (MA5407) in ICC/IF

Product Details

MA5407

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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