Invitrogen

IL-2 Monoclonal Antibody (B-G5), Biotin

Product Details

M600B

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

B-G5

Immunogen

Recombinant human IL-2

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223575

Product Specific Information

M600B targets IL-2 in ELISA, and WB applications and shows reactivity with Human samples.

The M600B immunogen is recombinant human IL-2.

M600B detects IL-2 which has a predicted molecular weight of approximately 15 kDa.

The M600B IL2 antibody (biotinylated conjugate of clone BG5) has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody P600. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M600B (clone BG5) and antibody P600 have successfully been used in combination with recombinant IL2 protein SIL2 in ELISA applications.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CARbodies: Human Antibodies Against Cell Surface Tumor Antigens Selected From Repertoires Displayed on T Cell Chimeric Antigen Receptors.

Molecular therapy. Nucleic acids

Alonso-Camino V,Sánchez-Martín D,Compte M,Nuñez-Prado N,Diaz RM,Vile R,Alvarez-Vallina L

M600B was used in ELISA to develop a system for the production of antibodies designated CARbodies with potential application in adoptive cell therapy

Tue May 21 00:00:00 EDT 2013

Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.

Neurobiology of aging

Loewenbrueck KF,Tigno-Aranjuez JT,Boehm BO,Lehmann PV,Tary-Lehmann M

M600B was used in ELISA to study the role of Th1 responses to beta-amyloid in Down syndrome and Alzheimer disease

Fri Oct 01 00:00:00 EDT 2010

Cell-mediated immune responses and protective efficacy against infection with Mycobacterium tuberculosis induced by Hsp65 and hIL-2 fusion protein in mice.

Scandinavian journal of immunology

Shi C,Yuan S,Zhang H,Zhang T,Wang L,Xu Z

M600B was used in western blot to study the ability of a Mycobacterium HSP65-human IL-2 fusion protein to induce protection against infection with Mycobacterium tuberculosis

Sun Feb 01 00:00:00 EST 2009

Lymphocyte display: a novel antibody selection platform based on T cell activation.

PloS one

Alonso-Camino V,Sánchez-Martín D,Compte M,Sanz L,Alvarez-Vallina L

M600B was used in ELISA to show that Jurkat alpha CEA-CIR-EGFP cells could be activated to secrete interleukin-2

Thu Sep 24 00:00:00 EDT 2009

Growth and differentiation advantages of CD4+ OX40+ T cells from allogeneic hematopoietic stem cell transplantation recipients.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Shindo T,Ishikawa T,Fukunaga A,Hori T,Uchiyama T

M600B was used in ELISA to investigate the growth and differentiation advantages of CD4+ OX40+ T cells from allogeneic hematopoietic stem cell transplantation recipients

Sat Mar 01 00:00:00 EST 2008

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

M600B was used in ELISA to study the production of granzyme B in newly activated CD8+ memory cells

Tue May 01 00:00:00 EDT 2007

Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.

AIDS (London, England)

Kuekrek H,Schlingmann T,Valdez H,Boehm BO,Pollard RB,Mitsuyasu R,Goebel FD,Lederman MM,Lehmann PV,Tary-Lehmann M

M600B was used in ELISA to study the usefulness of interleukin 2 treatment in HIV infected individuals

Fri Nov 18 00:00:00 EST 2005

IL-2 absorption affects IFN-gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.

Cellular immunology

Quast S,Zhang W,Shive C,Kovalovski D,Ott PA,Herzog BA,Boehm BO,Tary-Lehmann M,Karulin AY,Lehmann PV

M600B was used in ELISA to study the role of interleukin-2 absorption in interferon-gamma and interleukin-5 producing memory T cells

Thu Sep 01 00:00:00 EDT 2005

Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.

Journal of immunological methods

Guerkov RE,Targoni OS,Kreher CR,Boehm BO,Herrera MT,Tary-Lehmann M,Lehmann PV,Schwander SK

M600B was used in ELISA to investigate the expression of low-frequency antigen-specific interleukin-10-producing CD4+ T cells in peripheral blood mononuclear cells

Fri Aug 01 00:00:00 EDT 2003

CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.

Journal of immunological methods

Kreher CR,Dittrich MT,Guerkov R,Boehm BO,Tary-Lehmann M

M600B was used in ELISA to investigate the function of CD4+ and CD8+ cells in cryopreserved human peripheral blood mononuclear cells

Tue Jul 01 00:00:00 EDT 2003

Immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients.

AIDS (London, England)

Valdez H,Anthony D,Farukhi F,Patki A,Salkowitz J,Heeger P,Peterson DL,Post AB,Asaad R,Lederman MM

M600B was used in ELISA to investigate the immune responses to hepatitis C and non-hepatitis C antigens in hepatitis C virus infected and HIV-1 coinfected patients

Fri Oct 20 00:00:00 EDT 2000

Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.

Journal of immunology (Baltimore, Md. : 1950)

Heeger PS,Greenspan NS,Kuhlenschmidt S,Dejelo C,Hricik DE,Schulak JA,Tary-Lehmann M

M600B was used in ELISA to study the effect of donor memory lymphocytes in transplant rejection and survival

Sun Aug 15 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: aldesleukin; H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; involved in regulation of T-cell clonal expansion; M-IL-2; T cell growth factor; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: IL-2; IL2; lymphokine; TCGF

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UniProt ID: (Human) P60568

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Entrez Gene ID: (Human) 3558

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Function(s)

Ras guanyl-nucleotide exchange factor activity cytokine activity interleukin-2 receptor binding growth factor activity kinase activator activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding

Process(es)

Performance Guarantee

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IL-2 Monoclonal Antibody (B-G5), Biotin

Product Details

M600B

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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