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IRAK1 Antibody (700025) in IHC (P)
Immunohistochemistry analysis of IRAK1 showing staining in the cytoplasm of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, ... View More
Product Details
700025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
19H32L12
Immunogen
A peptide corresponding to amino acids 579-591 of human IRAK1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532270
Product Specific Information
This antibody is predicted to react with mouse based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. It is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. IRAK's association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Alternatively, IRAK1 can phosphorylate TIRAP to promote its ubiquitination and subsequent degradation. IRAK1 also phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus. This results in the transcriptional activiation of type I IFN genes, which drive the cell in an antiviral state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interleukin-1 receptor-associated kinase 1; interleukin-1 receptor-associated kinase-1; IRAK; IRAK-1; mPLK; Pelle homolog; Pelle-like protein kinase
Gene Aliases: AA408924; Il1rak; IRAK; IRAK-1; IRAK1; IRAK1-S; mPLK; pelle; Plpk
UniProt ID: (Human) P51617, (Mouse) Q62406
Entrez Gene ID: (Human) 3654, (Mouse) 16179
protein kinase activity
protein serine/threonine kinase activity
NF-kappaB-inducing kinase activity
protein binding
ATP binding
kinase activity
heat shock protein binding
protein homodimerization activity
protein heterodimerization activity
nucleotide binding
interleukin-1 receptor binding
transferase activity
transferase activity, transferring phosphorus-containing groups
activation of MAPK activity
regulation of cytokine-mediated signaling pathway
toll-like receptor signaling pathway
MyD88-dependent toll-like receptor signaling pathway
protein phosphorylation
signal transduction
transmembrane receptor protein serine/threonine kinase signaling pathway
activation of NF-kappaB-inducing kinase activity
JNK cascade
aging
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
positive regulation of type I interferon production
response to lipopolysaccharide
toll-like receptor 2 signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 9 signaling pathway
cellular response to heat
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAP kinase activity
innate immune response
positive regulation of transcription, DNA-templated
protein autophosphorylation
positive regulation of smooth muscle cell proliferation
positive regulation of NF-kappaB transcription factor activity
protein oligomerization
nucleotide-binding oligomerization domain containing signaling pathway
interleukin-1-mediated signaling pathway
response to interleukin-1
cellular response to hypoxia
immune system process
phosphorylation
cytokine-mediated signaling pathway
response to peptidoglycan
intracellular signal transduction
positive regulation of JUN kinase activity
negative regulation of transcription, DNA-templated
negative regulation of cholesterol efflux
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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