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Invitrogen
JAK3 Monoclonal Antibody (5H2)
Product Details
MA5-15561
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5H2
Immunogen
Purified recombinant fragment of human JAK3 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10980143
Product Specific Information
MA5-15561 targets JAK3 in indirect ELISA, FACS, IF, and WB applications and shows reactivity with Human and mouse samples.
The MA5-15561 immunogen is purified recombinant fragment of human JAK3 expressed in E. Coli.
MA5-15561 detects JAK3 which has a predicted molecular weight of approximately 125kDa.
Target Information
Janus kinase 3 (JAK3) phosphorylates members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to activation of interleukin receptors. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. However, active JAK1 may be required for complex responses. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Breast-JAK3; JAK-3; JAK3B; Janus kinase 3; Janus kinase 3 (a protein tyrosine kinase, leukocyte); L JAK; L-JAK; Leukocyte janus kinase; Tyrosine-protein kinase JAK3
Gene Aliases: fae; JAK-3; JAK3; JAK3_HUMAN; JAKL; L-JAK; LJAK; wil
UniProt ID: (Human) P52333, (Mouse) Q62137
Entrez Gene ID: (Human) 3718, (Mouse) 16453
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
receptor binding
protein binding
ATP binding
protein phosphatase binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
hormone receptor binding
MAPK cascade
adaptive immune response
negative regulation of dendritic cell cytokine production
protein phosphorylation
enzyme linked receptor protein signaling pathway
positive regulation of cytosolic calcium ion concentration
tyrosine phosphorylation of STAT protein
STAT protein import into nucleus
cell migration
peptidyl-tyrosine phosphorylation
B cell differentiation
negative regulation of interleukin-10 production
negative regulation of interleukin-12 production
intracellular signal transduction
interleukin-4-mediated signaling pathway
peptidyl-tyrosine autophosphorylation
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
tyrosine phosphorylation of Stat5 protein
T cell homeostasis
positive regulation of GTPase activity
innate immune response
negative regulation of FasL biosynthetic process
negative regulation of T-helper 1 cell differentiation
positive regulation of immune response
negative regulation of T cell activation
positive regulation of calcium ion transport
JAK-STAT cascade involved in growth hormone signaling pathway
regulation of T cell apoptotic process
negative regulation of thymocyte apoptotic process
response to interleukin-2
response to interleukin-4
response to interleukin-15
response to interleukin-9
immune system process
transmembrane receptor protein tyrosine kinase signaling pathway
phosphorylation
cytokine-mediated signaling pathway
erythrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
protein autophosphorylation
lymph node development
positive regulation of dendritic cell apoptotic process
positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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