Invitrogen

Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8)

Phospho-IRF3 (Ser396) Antibody in Western Blot (WB)

FIGURE: 1 / 1

Phospho-IRF3 (Ser396) Antibody (MA5-14947) in WB

Frontiers in microbiology 2022 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation of IRF-3 induced by PDCoV. LLC-PK cells were incubated with PDCoV for 1 h, and 2, 4, 8, and 16 muM of Se-Met was added. The expression of MAVS protein and the phosphorylation of IRF-3 were detected by western blot. Results were mean +- SD for three individual experiments.

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Product Details

MA5-14947

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Pig

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

E.875.8

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer396 of human IRF-3

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

46 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10987145

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antiviral Effect of Selenomethionine on Porcine Deltacoronavirus in Pig Kidney Epithelial Cells.

Frontiers in microbiology

Ren Z,Jia G,He H,Ding T,Yu Y,Zuo Z,Hu Y,Zhong Z,Yu S,Deng H,Shen L,Cao S,Peng G,Wang Y,Cai D,Gou L,Ma X,Liu H,Zhou Z,Deng Y,Yang D,Deng J

Published figure using Phospho-IRF3 (Ser396) monoclonal antibody (Product # MA5-14947) in Western Blot

Sat Mar 05 00:00:00 EST 2022

The Porcine Cyclic GMP-AMP Synthase-STING Pathway Exerts an Unusual Antiviral Function Independent of Interferon and Autophagy.

Journal of virology

Jiang S,Xia N,Luo J,Zhang Y,Cao Q,Zhang J,Wang Y,Zhao Y,Zheng W,Chen N,Meurens F,Li X,Zhu J

MA5-14947 was used in Western Blot to reveal a new layer of complexity in STING antiviral activity.

Wed Dec 14 00:00:00 EST 2022

Mechanism of selenomethionine inhibiting of PDCoV replication in LLC-PK1 cells based on STAT3/miR-125b-5p-1/HK2 signaling.

Frontiers in immunology

Ren Z,Ding T,He H,Wei Z,Shi R,Deng J

MA5-14947 was used in Western Blotting to provide a molecular basis for the development of new therapeutic approaches.

Thu Sep 08 00:00:00 EDT 2022

African Swine Fever Virus Structural Protein p17 Inhibits cGAS-STING Signaling Pathway Through Interacting With STING.

Frontiers in immunology

Zheng W,Xia N,Zhang J,Cao Q,Jiang S,Luo J,Wang H,Chen N,Zhang Q,Meurens F,Zhu J

MA5-14947 was used in Western Blotting to establish the role of p17 in the immune evasion and thus provides insights on African swine fever virus pathogenesis.

Tue Jul 19 00:00:00 EDT 2022

Porcine cGAS-STING signaling induced autophagy inhibits STING downstream IFN and apoptosis.

Frontiers in immunology

Xia N,Zheng W,Jiang S,Cao Q,Luo J,Zhang J,Xu Y,Sun S,Zhang K,Chen N,Meurens F,Zhu J

MA5-14947 was used in Western Blotting to systematically analyze the porcine STING (pSTING) induced IFN, autophagy and apoptosis, revealed the distinct dynamics of three STING downstream events, and established the IFN independent inductions of autophagy and apoptosis.

Tue Nov 01 00:00:00 EDT 2022

An autoinhibitory mechanism modulates MAVS activity in antiviral innate immune response.

Nature communications

Shi Y,Yuan B,Qi N,Zhu W,Su J,Li X,Qi P,Zhang D,Hou F

MA5-14947 was used in western blot to investigate regulatory mechanisms of MAVS activity

Fri Jul 17 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Interferon regulatory factor 3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3

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Gene Aliases: C920001K05Rik; IIAE7; IRF-3; IRF3

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UniProt ID: (Human) Q14653, (Mouse) P70671

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Entrez Gene ID: (Human) 3661, (Mouse) 54131

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription cofactor activity protein binding identical protein binding protein homodimerization activity regulatory region DNA binding transcription factor activity, sequence-specific DNA binding transcription regulatory region DNA binding winged helix/forkhead transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter apoptotic process cellular response to DNA damage stimulus lipopolysaccharide-mediated signaling pathway negative regulation of type I interferon production positive regulation of type I interferon production positive regulation of interferon-alpha production positive regulation of interferon-beta production TRIF-dependent toll-like receptor signaling pathway MDA-5 signaling pathway positive regulation of I-kappaB kinase/NF-kappaB signaling response to exogenous dsRNA type I interferon biosynthetic process positive regulation of cytokine secretion defense response to virus interferon-gamma-mediated signaling pathway type I interferon signaling pathway positive regulation of type I interferon-mediated signaling pathway cellular response to dsRNA macrophage apoptotic process programmed necrotic cell death immune system process transcription, DNA-templated regulation of transcription, DNA-templated response to bacterium response to lipopolysaccharide innate immune response negative regulation of interferon-beta biosynthetic process negative regulation of defense response to virus by host cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8)

Phospho-IRF3 (Ser396) Antibody (MA5-14947) in WB

Product Details

MA5-14947

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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